LOCUS       sequence-403399-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for human SCGB3A2 promoter-driven expression of 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   He P, Lim K, Sun D, Pett JP, Jeng Q, Polanski K, Dong Z, Bolt L, 
            Richardson L, Mamanova L, Dabrowska M, Wilbrey-Clark A, Madissoon E,
            Tuong ZK, Dann E, Suo C, Goh I, Yoshida M, Nikolic MZ, Janes SM, He 
            X, Barker RA, Teichmann SA, Marioni JC, Meyer KB, Rawlins EL
  TITLE     A human fetal lung cell atlas uncovers proximal-distal gradients of 
            differentiation and key regulators of epithelial fates.
  JOURNAL   Cell. 2022 Dec 8;185(25):4841-4860.e25. doi: 
            10.1016/j.cell.2022.11.005.
  PUBMED    36493756
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2022-12-8- 8";
            volume: "185"; issue: "25"; pages: "4841-4860.e25"
COMMENT     Sequence Label: pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2218..2442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2431..2448
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2470..3183)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      3180..3189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3224..3492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3505..3716)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4512..4521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4518..4520,4521..4523,4524..5237)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4518..4520,4521..4523,4524..5234)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4563..4584)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4824..4843)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5171..5192
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5245..5833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5298..5318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5716..5727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5908..6141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6260..6278)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6346..6450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6451..6519,6520..7311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6669..6688)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7482..8070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7971..7990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8224..8241
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8481..8502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8517..8522,8523..8540,8541..8547)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8555..8571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8560..8582
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8579..8595
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8579..8595
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8645..8664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     2281 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     2341 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     2401 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag
     2461 ctctagagct caattaagtt tgtgccccag tttgctaggg aggtcgcagt atctggccac
     2521 agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct
     2581 gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta
     2641 tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcgct
     2701 tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgag
     2761 tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt
     2821 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
     2881 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc
     2941 ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc tggtagccag
     3001 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
     3061 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt
     3121 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc ccttagacac
     3181 catggtggcg ttggctgcag gtgtaggcgc cggtcacagc ttggatctgt aacggcgcag
     3241 aacagaaaac gaaacaaaga cgtagagttg agcaagcagg gtcaggcaaa gcgtggagag
     3301 ccggctgagt ctaggtaggc tccaagggag cgccggacaa aggcccggtc tcgacctgag
     3361 ctttaaactt acctagacgg cggacgcagt tcaggaggca ccacaggcgg gaggcggcag
     3421 aacgcgactc aaccggcgtg gatggcggcc tcaggtaggg cggcgggcgc gtgaaggaga
     3481 gatgcgagcc cctcgaagct tcagctgtgt tctggcggca aacccgttgc gaaaaagaac
     3541 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     3601 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg cacccgttca
     3661 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     3721 acgggcaccg gagcgatcgc agatccttga cttaaatgcg gtatttctcg aaattgaatc
     3781 ccaggttttt caaaagacac tctgatttta gatcttaagc ctattattct atccaaaggc
     3841 tcagagaaga catgctctga acatactcaa agtaactgac actggaaaag gtaacagagg
     3901 tgtgaaaatc ttaccatagt agatgtgtga gtgggctggg ggcaggtgaa ttccagagaa
     3961 atgtggcact aaatgtcatg aagctgactc tgtattatac cagagtggcc tccagattgc
     4021 tttcacaact gggtagttca tcagctaatg tgattctcca aactttaaat gattagaaaa
     4081 ctggggaaaa atgtagaaaa ccagaggaaa acactccttc taatccaaat ataaattctt
     4141 cacttctttt caatgttctt ccaggagaag gattcgttgg gctctttgcc ttctgctttt
     4201 atttctgtgc aagggtttat gcaagaggta cttgagaatg ctgtactgta gagctttgtt
     4261 tctcatggga acacacgggg aagtggaaaa ccctccaaat tgtttggtga gaaaacataa
     4321 catttatccc tttctttggt ggggtgagtc aagtggtagg gactagaatt caggtcctca
     4381 atgggcatat aaatatgtgt gtgcaaaagc agccatcaca ctttgtatgg caagtggaac
     4441 cactggcttg gtggattttg ctagattttt ctgattttta aactcctgaa aaatatccca
     4501 gataactgtc ggccgccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4561 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4621 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4681 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4741 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4801 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4861 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4921 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4981 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5041 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5101 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5161 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5221 acgagctgta caagtaagga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     5281 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5341 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5401 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5461 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5521 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5581 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5641 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5701 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5761 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5821 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     5881 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5941 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6001 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6061 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6121 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6181 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     6241 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6301 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6421 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6481 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6541 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6601 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6661 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6721 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6781 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6841 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6901 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6961 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7021 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7081 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7141 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7201 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7261 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7321 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7381 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7441 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7501 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7561 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7621 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7681 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7741 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7801 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7861 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7921 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7981 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8041 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8101 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8161 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8221 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8281 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     8341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8401 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     8461 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8521 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8581 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     8641 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8701 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8761 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8821 tgtatttgtt aaatatgtac tacaaactta gtagt
//
