LOCUS       sequence-403447-       10587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_MetRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..984
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(244..263)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     854..873
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1024..1276
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1369..1393
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1914..2705
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2951..3539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3440..3459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3693..3710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3725..3865
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3791..3810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3951..3973
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4209..4403
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4469..5542)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5971..6600)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7931..7955
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8648..8992
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8936..8956
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9092..9116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9447..9471)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9479..9481,9482..9484,9485..10198)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9479..9481,9482..9484,9485..10195)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9524..9545)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9785..9804)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10132..10153
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10564..10587,1..156)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
       61 cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc
      121 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaactc
      181 gatcgaggca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      241 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      301 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      361 aatgaactcc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      421 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      481 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      541 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
      601 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
      661 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg
      721 atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg
      781 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
      841 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
      901 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
      961 cgccttcttg acgagttctt ctgagcggga ctctggggtt cggactctag ctagagtcaa
     1021 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     1081 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     1141 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     1201 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     1261 tctatgttac tagatcgacc ggcatgcaag ctgataattc aattcggcgt taattcagta
     1321 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     1381 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     1441 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     1501 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     1561 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     1621 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1681 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1741 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1801 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1861 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     1921 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1981 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2041 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2101 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2161 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2221 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2281 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2341 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2401 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2461 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2521 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2581 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2641 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2701 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     2761 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     2821 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     2881 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3061 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3421 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3481 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3541 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3601 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3661 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3721 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     3781 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     3841 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     3901 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3961 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     4021 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     4081 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     4141 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     4201 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     4261 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     4321 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     4381 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     4441 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     4501 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     4561 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     4621 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     4681 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     4741 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     4801 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     4861 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     4921 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     4981 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     5041 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     5101 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     5161 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     5221 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     5281 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     5341 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     5401 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     5461 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     5521 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     5581 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     5641 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     5701 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     5761 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     5821 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     5881 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     5941 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     6001 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     6061 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     6121 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     6181 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     6241 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     6301 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     6361 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     6421 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     6481 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     6541 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     6601 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     6661 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     6721 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
     6781 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
     6841 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
     6901 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
     6961 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
     7021 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
     7081 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
     7141 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
     7201 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
     7261 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
     7321 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
     7381 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
     7441 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
     7501 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
     7561 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
     7621 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
     7681 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
     7741 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa
     7801 agtactttaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
     7861 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
     7921 tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc
     7981 gggaaacgac aatctgatcc aagctcaagc taagcttgag ctctcccata tggtcgacta
     8041 gagccaagct gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg
     8101 atctaggaag aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga
     8161 agattagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg
     8221 cggcaggtct catcaagacg atctacccga gtaataatct ccaggagatc aaataccttc
     8281 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     8341 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     8401 ataaaccaag gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa
     8461 aggccatgga gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg
     8521 aacagttcat acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
     8581 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
     8641 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     8701 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
     8761 atcattgcga taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag
     8821 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     8881 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     8941 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg actccggtat
     9001 ttttacaaca attaccacaa caaaacaaac aacaaacaac attacaattt actattctag
     9061 tcgacctgca ggcggccgca ctagtgatat cacaagtttg tacaaaaaag caggctccgc
     9121 ggccgccccc ttcaccatgg ctagatctag agctaatgtt cttgcttctt ctcattctaa
     9181 ttctaaggtt caaactagat ctactcattc tagatctctt aagcttcttg aatctgttat
     9241 tgttggaact ggacaaaagt ctgatggatg tagagctcat aagagagctg ctattgatgg
     9301 agcttattct gctgctgctt atcttccaaa gtctcttctt attcaaagac atggaaagca
     9361 tgctaagact actatgggac atgttccact ttctaataat gctactgctc aatgttggac
     9421 tgaagctaag aagggtgggc gcgccgaccc agctttcttg tacaaagtgg tgatatcaat
     9481 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     9541 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     9601 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     9661 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     9721 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     9781 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     9841 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     9901 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     9961 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
    10021 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
    10081 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
    10141 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaacc
    10201 gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta
    10261 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
    10321 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
    10381 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac
    10441 gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa
    10501 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
    10561 acagaaccgc aacgttgaag gagccac
//
