LOCUS       sequence-403448-       10482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      78..666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     567..586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     820..837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    852..992
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(918..937)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1078..1100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1336..1530
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1596..2669)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3098..3727)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5058..5082
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        5775..6119
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6063..6083
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    6219..6243
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6469..6493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6501..6503,6504..6506,6507..7220)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6501..6503,6504..6506,6507..7217)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6546..6567)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6807..6826)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7154..7175
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        7586..7765
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7799..8593
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7853..7872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8463..8482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8633..8885
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8978..9002
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9523..10314
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
       61 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
      241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      721 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      781 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      841 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      901 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
      961 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     1021 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1081 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
     1141 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     1201 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     1261 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     1321 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     1381 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     1441 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     1501 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     1561 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     1621 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     1681 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     1741 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     1801 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     1861 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     1921 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     1981 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     2041 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     2101 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     2161 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     2221 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     2281 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     2341 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     2401 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     2461 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     2521 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     2581 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     2641 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     2701 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     2761 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     2821 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     2881 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     2941 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     3001 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     3061 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     3121 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     3181 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     3241 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     3301 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     3361 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
     3421 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
     3481 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     3541 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     3601 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     3661 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     3721 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     3781 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     3841 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     3901 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
     3961 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
     4021 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
     4081 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
     4141 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
     4201 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
     4261 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
     4321 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
     4381 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
     4441 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
     4501 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
     4561 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
     4621 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
     4681 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
     4741 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
     4801 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
     4861 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt
     4921 actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
     4981 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
     5041 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
     5101 tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc tcccatatgg
     5161 tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga ctttctctat
     5221 ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt caccaccgat
     5281 aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat ggttagagag
     5341 gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca ggagatcaaa
     5401 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     5461 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     5521 ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt agttcctact
     5581 gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact cgccgtgaag
     5641 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
     5701 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     5761 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     5821 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     5881 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc cgacagtggt
     5941 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     6001 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     6061 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact
     6121 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
     6181 attctagtcg acctgcaggc ggccgcacta gtgatatcac aagtttgtac aaaaaagcag
     6241 gctccgcggc cgcccccttc accatgaccg ttttctcagt tcagtccact atcttcagtc
     6301 gagcctccgt agctcttctc tcgagcaatg gcttcaaacg attttcattc gtttcttcgt
     6361 tttcttcctc cgccgcttac tctccaccta aaatgaggaa gcgtcgctac ccaatcgtct
     6421 ctgctgttga tattggtggc gtcgcaatcg ctaagggtgg gcgcgccgac ccagctttct
     6481 tgtacaaagt ggtgatatca atggtgagca agggcgagga gctgttcacc ggggtggtgc
     6541 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6601 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6661 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     6721 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6781 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6841 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6901 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6961 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     7021 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     7081 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     7141 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     7201 tggacgagct gtacaagtaa ccgcggccat gctagagtcc gcaaaaatca ccagtctctc
     7261 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
     7321 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     7381 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     7441 cagtgacctg caggcatgcg acgtcgggcc ctctagagga tccccgggta ccgcgaatta
     7501 tcgatcatga gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca
     7561 agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact gagccgcggg
     7621 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
     7681 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
     7741 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaactcg atcgaggcat
     7801 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7861 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7921 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     7981 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8041 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8101 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8161 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8221 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8281 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg
     8341 cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8401 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8461 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8521 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8581 cgagttcttc tgagcgggac tctggggttc ggactctagc tagagtcaag cagatcgttc
     8641 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     8701 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     8761 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     8821 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     8881 agatcgaccg gcatgcaagc tgataattca attcggcgtt aattcagtac attaaaaacg
     8941 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     9001 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     9061 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     9121 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     9181 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     9241 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     9301 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     9361 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     9421 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     9481 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     9541 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     9601 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     9661 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     9721 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     9781 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     9841 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     9901 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     9961 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
    10021 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
    10081 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
    10141 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
    10201 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
    10261 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
    10321 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
    10381 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
    10441 aagcgtgcat aataagccct acacaaattg ggagatatat ca
//
