LOCUS       sequence-403450-       10470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Agrostemma_githago _LysRS_MitoTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             195..989
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(249..268)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     859..878
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1029..1281
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1374..1398
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1919..2710
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2956..3544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3445..3464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3698..3715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3730..3870
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3796..3815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3956..3978
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4214..4408
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4474..5547)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5976..6605)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7936..7960
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8653..8997
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8941..8961
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9097..9121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9335..9359)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9367..9369,9370..9372,9373..10086)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9367..9369,9370..9372,9373..10083)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9412..9433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9673..9692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10020..10041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10452..10470,1..161)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
       61 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
      121 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
      181 aactcgatcg aggcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
      241 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
      301 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
      361 ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
      421 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
      481 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
      541 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
      601 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
      661 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
      721 cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata
      781 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
      841 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
      901 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
      961 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggac tctagctaga
     1021 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1081 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1141 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1201 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1261 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     1321 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     1381 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     1441 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     1501 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     1561 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     1621 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     1681 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     1741 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     1801 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     1861 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     1921 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     1981 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2041 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2101 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2161 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2221 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2281 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2341 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2401 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2461 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2521 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2581 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2641 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     2701 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     2761 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     2821 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     2881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3061 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3661 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3721 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3781 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     3841 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     3901 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3961 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     4021 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     4081 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     4141 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     4201 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     4261 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     4321 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     4381 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     4441 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     4501 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     4561 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     4621 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     4681 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     4741 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     4801 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     4861 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     4921 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     4981 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     5041 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     5101 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     5161 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     5221 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     5281 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     5341 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     5401 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     5461 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     5521 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     5581 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     5641 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     5701 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     5761 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     5821 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     5881 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     5941 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     6001 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     6061 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     6121 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     6181 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     6241 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     6301 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     6361 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     6421 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
     6481 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
     6541 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
     6601 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
     6661 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
     6721 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
     6781 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
     6841 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
     6901 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
     6961 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
     7021 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
     7081 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
     7141 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
     7201 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
     7261 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
     7321 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
     7381 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
     7441 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
     7501 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
     7561 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
     7621 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
     7681 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
     7741 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
     7801 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     7861 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     7921 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     7981 aaggcgggaa acgacaatct gatccaagct caagctaagc ttgagctctc ccatatggtc
     8041 gactagagcc aagctgatct cctttgcccc ggagatcacc atggacgact ttctctatct
     8101 ctacgatcta ggaagaaagt tcgacggaga aggtgacgat accatgttca ccaccgataa
     8161 tgagaagatt agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc
     8221 ctacgcggca ggtctcatca agacgatcta cccgagtaat aatctccagg agatcaaata
     8281 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
     8341 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
     8401 gcttcataaa ccaaggcaag taatagagat tggagtctct aagaaagtag ttcctactga
     8461 atcaaaggcc atggagtcaa aaattcagat cgaggatcta acagaactcg ccgtgaagac
     8521 tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa
     8581 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     8641 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     8701 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     8761 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcccctgccg acagtggtcc
     8821 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     8881 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     8941 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggactcc
     9001 ggtattttta caacaattac cacaacaaaa caaacaacaa acaacattac aatttactat
     9061 tctagtcgac ctgcaggcgg ccgcactagt gatatcacaa gtttgtacaa aaaagcaggc
     9121 tccgcggccg cccccttcac catgaacaaa atactacacc atatagtaaa acagcagccc
     9181 aaactcatat tccttcaacc aaaaaaaccc tcaacccttc ttcacttcac ccctatctcc
     9241 gccgcactcc ctcacaactc ctccaccttc tctctccgcc gtctaatgtc cacctccgac
     9301 gtcgacgccg ccgccgccaa gggtgggcgc gccgacccag ctttcttgta caaagtggtg
     9361 atatcaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     9421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     9481 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     9541 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     9601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     9661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     9721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     9781 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     9841 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     9901 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     9961 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
    10021 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
    10081 aagtaaccgc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
    10141 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
    10201 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
    10261 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg
    10321 catgcgacgt cgggccctct agaggatccc cgggtaccgc gaattatcga tcatgagcgg
    10381 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt
    10441 tggaactgac agaaccgcaa cgttgaagga
//
