LOCUS       sequence-403455-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. cerevisiae expression vector for SpuFz1 reprogrammed guide 
            (targeting PSP1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, 
            Desimone A, Macrae RK, Zhang F
  TITLE     Fanzor is a eukaryotic programmable RNA-guided endonuclease.
  JOURNAL   Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
  PUBMED    37380027
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2023 Jun 28. doi: 10.1038/s41586-023-06356-2."
COMMENT     Sequence Label: pMJ123: pRS426-His-MBP-SpuFz1_PSP1
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3666..3690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3821..3938
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4119..4142
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             4260..4289
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4299..5399
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5373..5396
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5454..5474
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(8076..8100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8148..8167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8149..8167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8177..8194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8177..8193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8186..8208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8334..8789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8421..8440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8631..8652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8920..9723)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9724..9939)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9963..9982)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10082..10104
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ccaccgcggt ggcggccccc gtcccaccat cgggcgcgga
     3661 tcatcacaag tttgtacaaa aaagcaggct ctttggccat ataggccacc aggtcctgca
     3721 ggcgcgctct tcaggaggcg cgccaactgc cctcttaatt aacgaagaag atcggaccgg
     3781 ggccgcggat ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc
     3841 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     3901 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     3961 tagcttttat ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg
     4021 aacgaatcaa attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct
     4081 gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc
     4141 tgtaaatcta tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt
     4201 ttaaaacacc aagaacttag tttcgaataa acacacataa ataaaccatg aaatcttctc
     4261 accatcacca tcaccatcac catcaccatg gttcttctat gaaaatcgaa gaaggtaaac
     4321 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
     4381 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
     4441 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
     4501 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
     4561 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
     4621 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
     4681 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
     4741 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
     4801 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
     4861 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
     4921 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     4981 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     5041 tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta
     5101 acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg
     5161 atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt
     5221 acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag
     5281 gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg
     5341 tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta
     5401 attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc gaggaaaacc
     5461 tgtacttcca atccaatgca ccgccaaaga agaagcaaaa gcttgaaagg ctcaaaaaac
     5521 tcgacaaacc caccttacat acctgcaaca agacgtcctt tgcaaaggct ttcttgccta
     5581 atgagaccta ccggcagcgt cttctagatt acatcgccat catccatcaa ctcgctgacc
     5641 atgcttccca cgctctcaaa ttctacatcc tctctacatc cacatcgtct ttcccggttg
     5701 ttcacgaaga tacaatcgaa gccattctct atcttctcaa caagggggag gcttggcatc
     5761 caagaaagga agcaaagaag gcatggcggg attgtttgtt gccatatgtt caacggtact
     5821 gccaaatcgt cggcttcatt catccaaatc tacgaggcga gcagcagtca attaattacc
     5881 tgacagtgag tatgatgacg aacctgaaag tcaacgttca agagcatttc atgcagatgc
     5941 tgctccggta cattaatctg cggttcgacg tgaagggaca gaagcagcga ctgccaccga
     6001 aaagcgatgc gcgaaaggct ttctttactc ggctacgcta cttgaagtcg gttttccttt
     6061 ttgatgtggt gcccgagttg gagttcctgg atgacttgac accattggag agcgaggttc
     6121 ttgaagagat ttggtcattg gatctcccat tccttcctaa cgatcccctt gcctacgcca
     6181 ttgttgctga tccgatgtca ttttttcccg cgtactgcaa actctctggg ctgtatgaac
     6241 aatatggctt ccaacggttc agtgcgatac ctctgcgccg ctcgctcatt caaagtcacg
     6301 tgcgaatcga caccatcatc ctgtaccagc atatcctttg catcacgcgg cgagacgcag
     6361 aaactgtcga aaaggatgat ctgtggatgc gcgtctgcaa tctatgtacc aaggcttttc
     6421 ggtctcgctg tggtatgcat tttgaaggct caatcaccac ggatggagcc tcggtgtctg
     6481 tatacctaaa gcacccagaa gcagacaagt atggaaagcg cggagcgaga aagtcagcaa
     6541 acacggtggc tgcagaggtt aaagcgcttt atgtggagaa caacttacct gcttgtcgag
     6601 cagcagagaa tgtcgtcgtt atcgacccaa acaagcgcga cattctctac tgccaagaca
     6661 gcaatggtac gacatttcgc tacaccgcca accagcgagc cgtggagact ggaagccggc
     6721 ggtttgcaaa gagacgagaa gcaatgaagg aagaagctgg agttgatctc atagaatcac
     6781 gaataccatc ccacaaaaca atgaacctca tggacttcac tcgctacctt ctcgttcgcc
     6841 gcgctgattg ggatcgtcgc aaggaattct attcgcatcc tgctcatact cgatggaaat
     6901 ggcactcctt cataaaccgt cagaagagcg aatcagacct cataagtaac atgcgcaaca
     6961 agtacggtga aaactttact gttgtgatgg gtgattggag cgacgctggt cgaactgcga
     7021 gatttcagac ctcatcaaag acaaaaggct ggcgcaccct ctttaagcgc aaccgcattg
     7081 attgtttcct tctcgacgag tacaagacct catctgtttg ccctcgctgt tcctcatctg
     7141 agtttgtgga gaaaaaattc aaaacacgac ctcattcaag accttggcgc cgtcgtgaag
     7201 gcaagattga aaaagtccac ggactgctgg gttgtaccaa ccctaactgt ttgcagcaag
     7261 cctggacatc gggaatgcgc tactggaatc gagatatgct gtcaacctgc aacatgctat
     7321 tgattgtgag atcaatgttg gatggacacg gtagaccaga agtgttcagc aggagtgttc
     7381 cagccgtagc gtagaacgta gattggttag attaggtttt ccgagccggt tgtcgcgcgg
     7441 ttcaatccct ggtgcgggtg ctagtgccaa tacccaccgg ctccgcacta gagaagtcat
     7501 ttaataaggc cactgttaaa aatacatcaa tgagacgagt gccctggtgc aagatctacc
     7561 atctgcacta ctcttctcaa taatcttgcc attaattatc ttagcagacc aactattccg
     7621 tttgccttct ttgggccaca accgcggttg cctgaggcat catatcggga aaccatttcg
     7681 gccatacata tccatacatg acaacccaaa aagccaagcc tgcaattgtt cctgcgctta
     7741 agtcgacttt gttcccactg tacttttagc tcgtacaaaa tacaatatac ttttcatttc
     7801 tccgtaaaca acatgttttc ccatgtaata tccttttcta tttttcgttc cgttaccaac
     7861 tttacacata ctttatatag ctattcactt ctatacacta aaaaactaag acaattttaa
     7921 ttttgctgcc tgccatattt caatttgtta taaattccta taatttatcc tattagtagc
     7981 taaaaaaaga tgaatgtgaa tcgaatccta agagaattcc cctcgaagat cgatctggcg
     8041 gcccggccgg ccgcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt
     8101 gatagcttgt cgagaagtac tcgagggggg gcccggtacc caattcgccc tatagtgagt
     8161 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8221 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8281 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     8341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8761 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     8821 tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg
     8881 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     8941 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     9001 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     9061 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     9121 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     9181 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     9241 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     9301 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     9361 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     9421 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     9481 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     9541 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     9601 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     9661 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     9721 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     9781 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     9841 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     9901 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc
     9961 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    10021 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10081 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc ga
//
