LOCUS       sequence-403457-        8698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Tetracyline selectable marker and tagRFP 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC093
FEATURES             Location/Qualifiers
     source          1..8698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(689..694,695..711,712..717)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             765..1955
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1117..1136)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    complement(2035..2082)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2110..2115,2116..2131,2132..2138)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2146..2162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      2915..3009
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(3158..3163,3164..3181,3182..3188)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3242..3901
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3308..3327)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4243..4548
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4427..4446
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4702..4867
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4910..5355
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5356..5442,5443..6777)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6860..6954
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(7075..7094)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7086..7901
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            8068..8176
                     /note="incP origin of transfer"
     rep_origin      8187..8575
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8606..8692)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgaagaat tctcatgttt gacagcttat catcgataag ctttaatgcg
      721 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
      781 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
      841 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
      901 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
      961 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1021 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1081 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1141 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1201 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1261 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1321 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1381 cgcggggcat gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag
     1441 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1501 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1561 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1621 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     1681 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     1741 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1801 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1861 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1921 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     1981 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg gcttgaagtt
     2041 cctatacttt ctagagaata ggaacttcgg aataggtact tccgctacta gaattcgagc
     2101 ggggaatttt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
     2161 aattttcaca caggaaacag ctaaatggtg agtaaaggtg aagagttgat taaagagaac
     2221 atgcatatga agttatacat ggagggaact gtcaataatc accactttaa gtgtacatca
     2281 gagggtgagg gaaagccata cgagggaacc caaactatga gaatcaaagt agtggaggga
     2341 ggtcctcttc catttgcttt tgatatacta gcaacaagtt tcatgtatgg ttccaggacc
     2401 ttcattaacc atactcaggg aatccctgac ttctttaaac agtcttttcc tgaaggtttt
     2461 acatgggaga gggttaccac ttacgaggac ggtggagtct tgacagcaac ccaggacact
     2521 tcattacaag atggatgcct aatatacaat gtgaaaatta ggggtgtgaa tttccctagt
     2581 aacggaccag ttatgcagaa gaaaacacta ggttgggaag ctaatactga aatgttgtac
     2641 cctgccgacg gaggtttaga aggtagatcc gacatggccc ttaagctagt cggaggtgga
     2701 cacttgatct gtaactttaa aaccacatat aggtctaaga agccagcaaa gaatctaaaa
     2761 atgcctggtg tttactatgt ggaccataga ctagaaagga taaaagaagc agacaaagaa
     2821 acttacgtgg agcaacatga ggtcgccgtc gctaggtatt gcgacttacc ttccaagcta
     2881 ggtcacaaat tgaacgctta attagctgag cttggactcc tgttgataga tccagtaatg
     2941 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     3001 ggtgagaatc tcccgcttta cgaattccca tggggagcat gcggagttac gtcatcccca
     3061 ccttccgctg cgttcttcat cgatgcccgc ttagtgatat gcttaaaact ccaagcacag
     3121 ccttctcccc ggggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     3181 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     3241 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     3301 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     3361 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     3421 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     3481 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     3541 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     3601 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     3661 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     3721 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     3781 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     3841 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     3901 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     3961 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     4021 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     4081 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     4141 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     4201 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     4261 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     4321 acgcccggac gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     4381 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     4441 gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct cagccaccgc
     4501 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     4561 cttatacaca gcccccgggg atgctggttg tcattgcccc tccttcctct aagtgataag
     4621 gttacttcct ctaaatgacc aagcagggta tctaatcctg ttaacgctcg ctcagagaat
     4681 tcgcatgcgg agtcgaggga gaaccagata agtgaaatct agttccaaac tattttgtca
     4741 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     4801 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     4861 gcccacacgc tgagcgaatt ccacgcttgt ggaattcctc gagggagttc acatatacct
     4921 gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt gtgattaaaa
     4981 aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga aaagaaacag
     5041 atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa
     5101 caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa
     5161 agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt gtaaagggca
     5221 aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt gtgcgtaact
     5281 aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca gtacataaaa
     5341 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
     5401 actaccgcac tgctggcagg aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag
     5461 ccatataagg aaacatacgg catttcccat attacacgcc atgatatgct gcaaatccct
     5521 gaacagcaaa aaaatgaaaa atatcaagtt cctgaattcg attcgtccac aattaaaaat
     5581 atctcttctg caaaaggcct ggacgtttgg gacagctggc cattacaaaa cgctgacggc
     5641 actgtcgcaa actatcacgg ctaccacatc gtctttgcat tagccggaga tcctaaaaat
     5701 gcggatgaca catcgattta catgttctat caaaaagtcg gcgaaacttc tattgacagc
     5761 tggaaaaacg ctggccgcgt ctttaaagac agcgacaaat tcgatgcaaa tgattctatc
     5821 ctaaaagacc aaacacaaga atggtcaggt tcagccacat ttacatctga cggaaaaatc
     5881 cgtttattct acactgattt ctccggtaaa cattacggca aacaaacact gacaactgca
     5941 caagttaacg tatcagcatc agacagctct ttgaacatca acggtgtaga ggattataaa
     6001 tcaatctttg acggtgacgg aaaaacgtat caaaatgtac agcagttcat cgatgaaggc
     6061 aactacagct caggcgacaa ccatacgctg agagatcctc actacgtaga agataaaggc
     6121 cacaaatact tagtatttga agcaaacact ggaactgaag atggctacca aggcgaagaa
     6181 tctttattta acaaagcata ctatggcaaa agcacatcat tcttccgtca agaaagtcaa
     6241 aaacttctgc aaagcgataa aaaacgcacg gctgagttag caaacggcgc tctcggtatg
     6301 attgagctaa acgatgatta cacactgaaa aaagtgatga aaccgctgat tgcatctaac
     6361 acagtaacag atgaaattga acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg
     6421 ttcactgact cccgcggatc aaaaatgacg attgacggca ttacgtctaa cgatatttac
     6481 atgcttggtt atgtttctaa ttctttaact ggcccataca agccgctgaa caaaactggc
     6541 cttgtgttaa aaatggatct tgatcctaac gatgtaacct ttacttactc acacttcgct
     6601 gtacctcaag cgaaaggaaa caatgtcgtg attacaagct atatgacaaa cagaggattc
     6661 tacgcagaca aacaatcaac gtttgcgcca agcttcctgc tgaacatcaa aggcaagaaa
     6721 acatctgttg tcaaagacag catccttgaa caaggacaat taacagttaa caaataaatt
     6781 cgctgagcga ggatgcacat gtgaccgagg gaagcttgcg gccgcgtcgt gactgggaaa
     6841 accctggcga ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat
     6901 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg
     6961 gtccccaata attacgattt aaatttgtgt ctcaaaatct ctgatgttac attgcacaag
     7021 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     7081 gtgttatgag ccatattcag cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca
     7141 tggatgcgga tctgtatggc tataaatggg cgcgtgataa cgtgggtcag agcggcgcga
     7201 ccatttatcg tctgtatggc aaaccggatg cgccggaact gtttctgaaa catggcaaag
     7261 gcagcgtggc gaacgatgtg accgatgaaa tggtgcgtct gaactggctg accgaattta
     7321 tgccgctgcc gaccattaaa cattttattc gcaccccgga tgatgcgtgg ctgctgacca
     7381 ccgcgattcc gggcaaaacc gcgtttcagg tgctggaaga atatccggat agcggcgaaa
     7441 acattgtgga tgcgctggcc gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact
     7501 gcccgtttaa cagcgatcgt gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg
     7561 gcctggtgga tgcgagcgat tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt
     7621 ggaaagaaat gcataaactg ctgccgttta gcccggatag cgtggtgacc cacggcgatt
     7681 ttagcctgga taacctgatt ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc
     7741 gtgtgggcat tgcggatcgt tatcaggatc tggccattct gtggaactgc ctgggcgaat
     7801 ttagcccgag cctgcaaaaa cgtctgtttc agaaatatgg cattgataat ccggatatga
     7861 acaaactgca atttcatctg atgctggatg aatttttcta ataattaatt ggaccgcggt
     7921 ccgcgcgttg tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
     7981 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     8041 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     8101 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     8161 tctgcgaggc tggccgtagg ccggccgatc tgaagatcag cagttcaacc tgttgatagt
     8221 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca
     8281 tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag
     8341 ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca
     8401 acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt
     8461 aaagccttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc
     8521 ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc tgacatgggg
     8581 cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
     8641 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcct
//
