LOCUS       sequence-403461-        8704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Tetracyline selectable marker and GFP 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC096
FEATURES             Location/Qualifiers
     source          1..8704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(689..694,695..711,712..717)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             765..1955
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1117..1136)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    complement(2035..2082)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2110..2115,2116..2131,2132..2138)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2146..2162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2185..2898
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2215..2243)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2838..2858
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2921..3015
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(3164..3169,3170..3187,3188..3194)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3248..3907
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3314..3333)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4249..4554
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4433..4452
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4708..4873
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4916..5361
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5362..5448,5449..6783)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6866..6960
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(7081..7100)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7092..7907
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            8074..8182
                     /note="incP origin of transfer"
     rep_origin      8193..8581
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8612..8698)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgaagaat tctcatgttt gacagcttat catcgataag ctttaatgcg
      721 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
      781 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
      841 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
      901 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
      961 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1021 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1081 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1141 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1201 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1261 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1321 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1381 cgcggggcat gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag
     1441 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1501 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1561 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1621 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     1681 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     1741 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1801 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1861 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1921 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     1981 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg gcttgaagtt
     2041 cctatacttt ctagagaata ggaacttcgg aataggtact tccgctacta gaattcgagc
     2101 ggggaattct tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
     2161 aattttcaca caggaaacag ctaaatgcgt aaaggagaag aacttttcac tggagttgtc
     2221 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     2281 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     2341 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
     2401 agatacccag atcatatgaa acagcatgac tttttcaaga gtgtcatgcc cgaaggttat
     2461 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     2521 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     2581 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     2641 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     2701 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     2761 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     2821 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     2881 atggatgaac tatacaaatg agcttaatta gctgagcttg gactcctgtt gatagatcca
     2941 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     3001 tttattggtg agaatctccc gctttacgaa ttcccatggg gagcatgcgg agttacgtca
     3061 tccccacctt ccgctgcgtt cttcatcgat gcccgcttag tgatatgctt aaaactccaa
     3121 gcacagcctt ctccccgggg cggccgcatt aggcacccca ggctttacac tttatgcttc
     3181 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
     3241 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     3301 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     3361 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     3421 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     3481 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     3541 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     3601 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     3661 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     3721 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     3781 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     3841 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     3901 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     3961 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     4021 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     4081 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     4141 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     4201 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     4261 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     4321 attgacacgc ccggacgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     4381 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     4441 accaccgata tggccagtgt gccggtatcc gttatcgggg aagaagtggc tgatctcagc
     4501 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     4561 ggctccctta tacacagccc ccggggatgc tggttgtcat tgcccctcct tcctctaagt
     4621 gataaggtta cttcctctaa atgaccaagc agggtatcta atcctgttaa cgctcgctca
     4681 gagaattcgc atgcggagtc gagggagaac cagataagtg aaatctagtt ccaaactatt
     4741 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     4801 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     4861 ttgtccgccc acacgctgag cgaattccac gcttgtggaa ttcctcgagg gagttcacat
     4921 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga
     4981 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag
     5041 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct
     5101 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg
     5161 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa
     5221 agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc
     5281 gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac
     5341 ataaaaaagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta
     5401 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac
     5461 caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa
     5521 atccctgaac agcaaaaaaa tgaaaaatat caagttcctg aattcgattc gtccacaatt
     5581 aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct
     5641 gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct
     5701 aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt
     5761 gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat
     5821 tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga
     5881 aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca
     5941 actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat
     6001 tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat
     6061 gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat
     6121 aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc
     6181 gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa
     6241 agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc
     6301 ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca
     6361 tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg
     6421 tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat
     6481 atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa
     6541 actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac
     6601 ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga
     6661 ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc
     6721 aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa
     6781 taaattcgct gagcgaggat gcacatgtga ccgagggaag cttgcggccg cgtcgtgact
     6841 gggaaaaccc tggcgactag tcttggactc ctgttgatag atccagtaat gacctcagaa
     6901 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     6961 ccaggggtcc ccaataatta cgatttaaat ttgtgtctca aaatctctga tgttacattg
     7021 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     7081 caaggggtgt tatgagccat attcagcgtg aaacgagctg tagccgtccg cgtctgaaca
     7141 gcaacatgga tgcggatctg tatggctata aatgggcgcg tgataacgtg ggtcagagcg
     7201 gcgcgaccat ttatcgtctg tatggcaaac cggatgcgcc ggaactgttt ctgaaacatg
     7261 gcaaaggcag cgtggcgaac gatgtgaccg atgaaatggt gcgtctgaac tggctgaccg
     7321 aatttatgcc gctgccgacc attaaacatt ttattcgcac cccggatgat gcgtggctgc
     7381 tgaccaccgc gattccgggc aaaaccgcgt ttcaggtgct ggaagaatat ccggatagcg
     7441 gcgaaaacat tgtggatgcg ctggccgtgt ttctgcgtcg tctgcatagc attccggtgt
     7501 gcaactgccc gtttaacagc gatcgtgtgt ttcgtctggc ccaggcgcag agccgtatga
     7561 acaacggcct ggtggatgcg agcgattttg atgatgaacg taacggctgg ccggtggaac
     7621 aggtgtggaa agaaatgcat aaactgctgc cgtttagccc ggatagcgtg gtgacccacg
     7681 gcgattttag cctggataac ctgattttcg atgaaggcaa actgattggc tgcattgatg
     7741 tgggccgtgt gggcattgcg gatcgttatc aggatctggc cattctgtgg aactgcctgg
     7801 gcgaatttag cccgagcctg caaaaacgtc tgtttcagaa atatggcatt gataatccgg
     7861 atatgaacaa actgcaattt catctgatgc tggatgaatt tttctaataa ttaattggac
     7921 cgcggtccgc gcgttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     7981 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     8041 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     8101 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     8161 tcctgctctg cgaggctggc cgtaggccgg ccgatctgaa gatcagcagt tcaacctgtt
     8221 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
     8281 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
     8341 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
     8401 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
     8461 gcttttaaag cctttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
     8521 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
     8581 atggggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     8641 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     8701 gcct
//
