LOCUS       sequence-403462-        8472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Kanamycin selectable marker and GFP 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC095
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(681..933)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(953..1504)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1546..1725)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    complement(1803..1850)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(1878..1883,1884..1899,1900..1906)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1914..1930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1953..2666
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1983..2011)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2606..2626
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2689..2783
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2932..2937,2938..2955,2956..2962)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3016..3675
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3082..3101)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4017..4322
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4201..4220
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4476..4641
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4684..5129
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5130..5216,5217..6551)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6634..6728
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6849..6868)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6860..7675
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7842..7950
                     /note="incP origin of transfer"
     rep_origin      7961..8349
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8380..8466)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc
      721 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
      781 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
      841 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
      901 taagaaactt tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt
      961 cggtgacggg caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac
     1021 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1081 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1141 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     1201 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     1261 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     1321 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     1381 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     1441 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     1501 tcatggtaga tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     1561 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     1621 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     1681 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     1741 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcagc
     1801 ttgaagttcc tatactttct agagaatagg aacttcggaa taggtacttc cgctactaga
     1861 attcgagcgg ggaattcttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
     1921 cggataacaa ttttcacaca ggaaacagct aaatgcgtaa aggagaagaa cttttcactg
     1981 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     2041 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     2101 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     2161 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gtcatgcccg
     2221 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     2281 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     2341 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     2401 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     2461 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     2521 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     2581 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     2641 cacatggcat ggatgaacta tacaaatgag cttaattagc tgagcttgga ctcctgttga
     2701 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
     2761 gggcgttttt tattggtgag aatctcccgc tttacgaatt cccatgggga gcatgcggag
     2821 ttacgtcatc cccaccttcc gctgcgttct tcatcgatgc ccgcttagtg atatgcttaa
     2881 aactccaagc acagccttct ccccggggcg gccgcattag gcaccccagg ctttacactt
     2941 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     3001 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     3061 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     3121 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     3181 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     3241 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     3301 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     3361 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3421 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3481 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     3541 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     3601 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3661 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     3721 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3781 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3841 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3901 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3961 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     4021 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     4081 gtgatattat tgacacgccc ggacgacgga tggtgatccc cctggccagt gcacgtctgc
     4141 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     4201 gcatgatgac caccgatatg gccagtgtgc cggtatccgt tatcggggaa gaagtggctg
     4261 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     4321 aaatgtcagg ctcccttata cacagccccc ggggatgctg gttgtcattg cccctccttc
     4381 ctctaagtga taaggttact tcctctaaat gaccaagcag ggtatctaat cctgttaacg
     4441 ctcgctcaga gaattcgcat gcggagtcga gggagaacca gataagtgaa atctagttcc
     4501 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     4561 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     4621 caactatttt gtccgcccac acgctgagcg aattccacgc ttgtggaatt cctcgaggga
     4681 gttcacatat acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg
     4741 aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga
     4801 atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat
     4861 gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata
     4921 gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa
     4981 atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt
     5041 tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc agaccgctaa
     5101 cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca aaacaagcaa
     5161 cagtattaac ctttactacc gcactgctgg caggaggcgc aactcaagcg tttgcgaaag
     5221 aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata
     5281 tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agttcctgaa ttcgattcgt
     5341 ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac
     5401 aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg
     5461 gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa
     5521 cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg
     5581 caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat
     5641 ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa
     5701 cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg
     5761 tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt
     5821 tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg
     5881 tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct
     5941 accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc
     6001 gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg
     6061 gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc
     6121 tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg
     6181 gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt
     6241 ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc
     6301 tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt
     6361 actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga
     6421 caaacagagg attctacgca gacaaacaat caacgtttgc gccaagcttc ctgctgaaca
     6481 tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag
     6541 ttaacaaata aattcgctga gcgaggatgc acatgtgacc gagggaagct tgcggccgcg
     6601 tcgtgactgg gaaaaccctg gcgactagtc ttggactcct gttgatagat ccagtaatga
     6661 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     6721 gtgagaatcc aggggtcccc aataattacg atttaaattt gtgtctcaaa atctctgatg
     6781 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     6841 cagtaataca aggggtgtta tgagccatat tcagcgtgaa acgagctgta gccgtccgcg
     6901 tctgaacagc aacatggatg cggatctgta tggctataaa tgggcgcgtg ataacgtggg
     6961 tcagagcggc gcgaccattt atcgtctgta tggcaaaccg gatgcgccgg aactgtttct
     7021 gaaacatggc aaaggcagcg tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg
     7081 gctgaccgaa tttatgccgc tgccgaccat taaacatttt attcgcaccc cggatgatgc
     7141 gtggctgctg accaccgcga ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc
     7201 ggatagcggc gaaaacattg tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat
     7261 tccggtgtgc aactgcccgt ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag
     7321 ccgtatgaac aacggcctgg tggatgcgag cgattttgat gatgaacgta acggctggcc
     7381 ggtggaacag gtgtggaaag aaatgcataa actgctgccg tttagcccgg atagcgtggt
     7441 gacccacggc gattttagcc tggataacct gattttcgat gaaggcaaac tgattggctg
     7501 cattgatgtg ggccgtgtgg gcattgcgga tcgttatcag gatctggcca ttctgtggaa
     7561 ctgcctgggc gaatttagcc cgagcctgca aaaacgtctg tttcagaaat atggcattga
     7621 taatccggat atgaacaaac tgcaatttca tctgatgctg gatgaatttt tctaataatt
     7681 aattggaccg cggtccgcgc gttgtccttt tccgctgcat aaccctgctt cggggtcatt
     7741 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     7801 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     7861 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     7921 aacgggaatc ctgctctgcg aggctggccg taggccggcc gatctgaaga tcagcagttc
     7981 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
     8041 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
     8101 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
     8161 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
     8221 tatataaggc ttttaaagcc tttaaggttt aacggttgtg gacaacaagc cagggatgta
     8281 acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag gaacacttaa
     8341 cggctgacat ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     8401 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     8461 tatttgatgc ct
//
