LOCUS       sequence-403464-        8432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP 
            fluorescent marker mPlum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC100
FEATURES             Location/Qualifiers
     source          1..8432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(681..933)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(953..1504)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1546..1725)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    complement(1803..1850)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(1877..1882,1883..1898,1899..1905)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1913..1929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1952..2635
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGAA"
     primer_bind     2547..2570
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      2649..2743
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2892..2897,2898..2915,2916..2922)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2976..3635
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3042..3061)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3977..4282
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4161..4180
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4436..4601
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4644..5089
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5090..5176,5177..6511)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6594..6688
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6809..6828)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6820..7635
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7802..7910
                     /note="incP origin of transfer"
     rep_origin      7921..8309
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8340..8426)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc
      721 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
      781 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
      841 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
      901 taagaaactt tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt
      961 cggtgacggg caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac
     1021 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1081 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1141 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     1201 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     1261 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     1321 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     1381 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     1441 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     1501 tcatggtaga tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     1561 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     1621 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     1681 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     1741 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcagc
     1801 ttgaagttcc tatactttct agagaatagg aacttcggaa taggtacttc cgctactaaa
     1861 ttcgagcggg gaattcttga caattaatca tcggctcgta taatgtgtgg aattgtgagc
     1921 ggataacaat tttcacacag gaaacagcta aatggtgagc aagggcgagg aggtcatcaa
     1981 ggagttcatg cgcttcaagg agcacatgga gggctccgtg aacggccacg agttcgagat
     2041 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaggc tgaaggtgac
     2101 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagatca tgtacggctc
     2161 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2221 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2281 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aaggtgcgcg gcaccaactt
     2341 cccctccgac ggccccgtaa tgcagaaaaa gaccatgggc tgggaggcct cctccgagcg
     2401 gatgtacccc gaggacggcg ccctgaaggg cgagatgaag atgaggctga ggctgaagga
     2461 cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct
     2521 gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac
     2581 catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgccg cttaattagc
     2641 tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     2701 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatctcccgc tttacgaatt
     2761 cccatgggga gcatgcggag ttacgtcatc cccaccttcc gctgcgttct tcatcgatgc
     2821 ccgcttagtg atatgcttaa aactccaagc acagccttct ccccggggcg gccgcattag
     2881 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     2941 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     3001 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     3061 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     3121 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     3181 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     3241 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     3301 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     3361 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     3421 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     3481 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3541 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3601 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     3661 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     3721 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     3781 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     3841 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     3901 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     3961 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     4021 tctgtttgtg gatgtacaga gtgatattat tgacacgccc ggacgacgga tggtgatccc
     4081 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     4141 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtatccgt
     4201 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     4261 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccccc ggggatgctg
     4321 gttgtcattg cccctccttc ctctaagtga taaggttact tcctctaaat gaccaagcag
     4381 ggtatctaat cctgttaacg ctcgctcaga gaattcgcat gcggagtcga gggagaacca
     4441 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     4501 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     4561 tttccacccc tcccagttcc caactatttt gtccgcccac acgctgagcg aattccacgc
     4621 ttgtggaatt cctcgaggga gttcacatat acctgccgtt cactattatt tagtgaaatg
     4681 agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga
     4741 aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg
     4801 tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag accagttgca
     4861 atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga
     4921 taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt
     4981 acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc
     5041 tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa
     5101 aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc
     5161 aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc
     5221 ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca
     5281 agttcctgaa ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt
     5341 ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca
     5401 catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt
     5461 ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa
     5521 agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc
     5581 aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg
     5641 taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag
     5701 ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac
     5761 gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac
     5821 gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa
     5881 cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg
     5941 caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg
     6001 cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact
     6061 gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc
     6121 gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat
     6181 gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt
     6241 aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc
     6301 taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt
     6361 cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc
     6421 gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct
     6481 tgaacaagga caattaacag ttaacaaata aattcgctga gcgaggatgc acatgtgacc
     6541 gagggaagct tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc ttggactcct
     6601 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     6661 ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg atttaaattt
     6721 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     6781 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcagcgtgaa
     6841 acgagctgta gccgtccgcg tctgaacagc aacatggatg cggatctgta tggctataaa
     6901 tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt atcgtctgta tggcaaaccg
     6961 gatgcgccgg aactgtttct gaaacatggc aaaggcagcg tggcgaacga tgtgaccgat
     7021 gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc tgccgaccat taaacatttt
     7081 attcgcaccc cggatgatgc gtggctgctg accaccgcga ttccgggcaa aaccgcgttt
     7141 caggtgctgg aagaatatcc ggatagcggc gaaaacattg tggatgcgct ggccgtgttt
     7201 ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt ttaacagcga tcgtgtgttt
     7261 cgtctggccc aggcgcagag ccgtatgaac aacggcctgg tggatgcgag cgattttgat
     7321 gatgaacgta acggctggcc ggtggaacag gtgtggaaag aaatgcataa actgctgccg
     7381 tttagcccgg atagcgtggt gacccacggc gattttagcc tggataacct gattttcgat
     7441 gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg gcattgcgga tcgttatcag
     7501 gatctggcca ttctgtggaa ctgcctgggc gaatttagcc cgagcctgca aaaacgtctg
     7561 tttcagaaat atggcattga taatccggat atgaacaaac tgcaatttca tctgatgctg
     7621 gatgaatttt tctaataatt aattggaccg cggtccgcgc gttgtccttt tccgctgcat
     7681 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     7741 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     7801 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     7861 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc
     7921 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
     7981 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
     8041 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct
     8101 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
     8161 ttttataaga aaaaaaagaa tatataaggc ttttaaagcc tttaaggttt aacggttgtg
     8221 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
     8281 agtgacacag gaacacttaa cggctgacat ggggcgcgcc cagctgtcta gggcggcgga
     8341 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     8401 ttcgactgag cctttcgttt tatttgatgc ct
//
