LOCUS       sequence-403467-        8221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP
            fluorescent marker mPlum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC086
FEATURES             Location/Qualifiers
     source          1..8221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(711..716,717..733,734..739)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             928..1461
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(1591..1638)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(1666..1671,1672..1687,1688..1694)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1702..1718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1741..2424
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGAA"
     primer_bind     2336..2359
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      2438..2532
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2681..2686,2687..2704,2705..2711)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2765..3424
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2831..2850)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3766..4071
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3950..3969
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4225..4390
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4433..4878
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(4879..4965,4966..6300)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6383..6477
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6598..6617)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6609..7424
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7591..7699
                     /note="incP origin of transfer"
     rep_origin      7710..8098
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8129..8215)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aagatcccct gattcccttt gtcaacagca atggatcgaa ttgacataag
      721 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      781 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      841 gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      901 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
      961 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg
     1021 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
     1081 ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc
     1141 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
     1201 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc
     1261 gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
     1321 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
     1381 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
     1441 gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc ggccgcggag
     1501 ttgttcggta aattgtcaca acgccgcggc caattcgatc tagaattcca ttgagtaagt
     1561 ttttaagcac atcagcttca aaagcgctct gaagttccta tactttctag agaataggaa
     1621 cttcggaata ggtacttccg ctactagaat tcgagcgggg aattcttgac aattaatcat
     1681 cggctcgtat aatgtgtgga attgtgagcg gataacaatt ttcacacagg aaacagctaa
     1741 atggtgagca agggcgagga ggtcatcaag gagttcatgc gcttcaagga gcacatggag
     1801 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1861 ggcacccaga ccgccaggct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1921 atcctgtccc ctcagatcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1981 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2041 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2101 tacaaggtga aggtgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaaaaag
     2161 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2221 gagatgaaga tgaggctgag gctgaaggac ggcggccact acgacgccga ggtcaagacc
     2281 acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg
     2341 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
     2401 cgccactcca ccggcgccgc ttaattagct gagcttggac tcctgttgat agatccagta
     2461 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2521 attggtgaga atctcccgct ttacgaattc ccatggggag catgcggagt tacgtcatcc
     2581 ccaccttccg ctgcgttctt catcgatgcc cgcttagtga tatgcttaaa actccaagca
     2641 cagccttctc cccggggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2701 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     2761 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2821 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2881 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2941 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     3001 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     3061 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     3121 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     3181 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     3241 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3301 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     3361 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     3421 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     3481 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     3541 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     3601 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3661 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3721 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     3781 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3841 gacacgcccg gacgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3901 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3961 accgatatgg ccagtgtgcc ggtatccgtt atcggggaag aagtggctga tctcagccac
     4021 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     4081 tcccttatac acagcccccg gggatgctgg ttgtcattgc ccctccttcc tctaagtgat
     4141 aaggttactt cctctaaatg accaagcagg gtatctaatc ctgttaacgc tcgctcagag
     4201 aattcgcatg cggagtcgag ggagaaccag ataagtgaaa tctagttcca aactattttg
     4261 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     4321 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     4381 tccgcccaca cgctgagcga attccacgct tgtggaattc ctcgagggag ttcacatata
     4441 cctgccgttc actattattt agtgaaatga gatattatga tattttctga attgtgatta
     4501 aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa
     4561 cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc
     4621 aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg
     4681 aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg
     4741 gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta
     4801 actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata
     4861 aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc
     4921 tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa
     4981 aagccatata aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc
     5041 cctgaacagc aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa
     5101 aatatctctt ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac
     5161 ggcactgtcg caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa
     5221 aatgcggatg acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac
     5281 agctggaaaa acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct
     5341 atcctaaaag accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa
     5401 atccgtttat tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact
     5461 gcacaagtta acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat
     5521 aaatcaatct ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa
     5581 ggcaactaca gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa
     5641 ggccacaaat acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa
     5701 gaatctttat ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt
     5761 caaaaacttc tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt
     5821 atgattgagc taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct
     5881 aacacagtaa cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac
     5941 ctgttcactg actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt
     6001 tacatgcttg gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact
     6061 ggccttgtgt taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc
     6121 gctgtacctc aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga
     6181 ttctacgcag acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag
     6241 aaaacatctg ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa
     6301 attcgctgag cgaggatgca catgtgaccg agggaagctt gcggccgcgt cgtgactggg
     6361 aaaaccctgg cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     6421 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     6481 ggggtcccca ataattacga tttaaatttg tgtctcaaaa tctctgatgt tacattgcac
     6541 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     6601 ggggtgttat gagccatatt cagcgtgaaa cgagctgtag ccgtccgcgt ctgaacagca
     6661 acatggatgc ggatctgtat ggctataaat gggcgcgtga taacgtgggt cagagcggcg
     6721 cgaccattta tcgtctgtat ggcaaaccgg atgcgccgga actgtttctg aaacatggca
     6781 aaggcagcgt ggcgaacgat gtgaccgatg aaatggtgcg tctgaactgg ctgaccgaat
     6841 ttatgccgct gccgaccatt aaacatttta ttcgcacccc ggatgatgcg tggctgctga
     6901 ccaccgcgat tccgggcaaa accgcgtttc aggtgctgga agaatatccg gatagcggcg
     6961 aaaacattgt ggatgcgctg gccgtgtttc tgcgtcgtct gcatagcatt ccggtgtgca
     7021 actgcccgtt taacagcgat cgtgtgtttc gtctggccca ggcgcagagc cgtatgaaca
     7081 acggcctggt ggatgcgagc gattttgatg atgaacgtaa cggctggccg gtggaacagg
     7141 tgtggaaaga aatgcataaa ctgctgccgt ttagcccgga tagcgtggtg acccacggcg
     7201 attttagcct ggataacctg attttcgatg aaggcaaact gattggctgc attgatgtgg
     7261 gccgtgtggg cattgcggat cgttatcagg atctggccat tctgtggaac tgcctgggcg
     7321 aatttagccc gagcctgcaa aaacgtctgt ttcagaaata tggcattgat aatccggata
     7381 tgaacaaact gcaatttcat ctgatgctgg atgaattttt ctaataatta attggaccgc
     7441 ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt
     7501 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
     7561 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
     7621 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
     7681 tgctctgcga ggctggccgt aggccggccg atctgaagat cagcagttca acctgttgat
     7741 agtacgtact aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc
     7801 tcatgtttaa cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact
     7861 aagctctcat gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca
     7921 gcaacttaaa tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct
     7981 tttaaagcct ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga
     8041 agcccttaga gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacatg
     8101 gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
     8161 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     8221 t
//
