LOCUS       sequence-403472-        8745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Tetracyline selectable marker and AmCyan1 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC092
FEATURES             Location/Qualifiers
     source          1..8745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(689..694,695..711,712..717)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             765..1955
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1117..1136)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    complement(2035..2082)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2109..2114,2115..2130,2131..2137)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2145..2161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2250..2259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2256..2948
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPFA"
     terminator      2962..3056
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(3205..3210,3211..3228,3229..3235)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3289..3948
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3355..3374)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4290..4595
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4474..4493
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4749..4914
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4957..5402
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5403..5489,5490..6824)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6907..7001
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(7122..7141)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7133..7948
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            8115..8223
                     /note="incP origin of transfer"
     rep_origin      8234..8622
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8653..8739)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgaagaat tctcatgttt gacagcttat catcgataag ctttaatgcg
      721 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
      781 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
      841 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
      901 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
      961 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1021 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1081 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1141 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1201 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1261 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1321 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1381 cgcggggcat gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag
     1441 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1501 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1561 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1621 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     1681 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     1741 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1801 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1861 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1921 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     1981 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg gcttgaagtt
     2041 cctatacttt ctagagaata ggaacttcgg aataggtact tccgctacta aattcgagcg
     2101 gggaattctt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
     2161 attttcacac aggaaacagc taaatgacca tgattacgcc aagcttgcat gcctgcaggt
     2221 cgactctaga ggatccacgg gtaccggtcg ccaccatggc tctttcaaac aagtttatcg
     2281 gagatgacat gaaaatgacc taccatatgg atggctgtgt caatgggcat tactttaccg
     2341 tcaaaggtga aggcagcggg aagccatacg aagggacgca gacctcgact tttaaagtca
     2401 ccatggccaa cggtgggccc cttgcattct cctttgacat actatctaca gtgttcatgt
     2461 atggaaatcg atgctttact gcgtatccta ccagtatgcc cgactatttc aaacaagcat
     2521 ttcctgacgg aatgtcatat gaaaggactt ttacctatga agatggagga gttgctacag
     2581 ccagttggga aataagcctt aaaggcaact gctttgagca caaatccacg tttcatggag
     2641 tgaactttcc tgctgatgga cctgtgatgg cgaagatgac aactggttgg gacccatctt
     2701 ttgagaaaat gactgtctgc gatggaatat tgaagggtga tgtcaccgcg ttcctcatgc
     2761 tgcaaggagg tggcaattac agatgccaat tccacacttc ttacaagaca aaaaaaccgg
     2821 tgacgatgcc accaaaccat gcggtggaac atcgcattgc gaggaccgac cttgacaaag
     2881 gtggcaacag tgttcagctg acggagcacg ctgttgcaca tataacctct gttgtccctt
     2941 tcgcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     3001 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatctcc
     3061 cgctttacga attcccatgg ggagcatgcg gagttacgtc atccccacct tccgctgcgt
     3121 tcttcatcga tgcccgctta gtgatatgct taaaactcca agcacagcct tctccccggg
     3181 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     3241 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     3301 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     3361 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     3421 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     3481 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     3541 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     3601 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     3661 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     3721 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3781 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3841 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     3901 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     3961 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     4021 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     4081 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     4141 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     4201 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     4261 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     4321 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccggacgac
     4381 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     4441 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     4501 tgccggtatc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     4561 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     4621 cccggggatg ctggttgtca ttgcccctcc ttcctctaag tgataaggtt acttcctcta
     4681 aatgaccaag cagggtatct aatcctgtta acgctcgctc agagaattcg catgcggagt
     4741 cgagggagaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     4801 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     4861 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacacgctga
     4921 gcgaattcca cgcttgtgga attcctcgag ggagttcaca tatacctgcc gttcactatt
     4981 atttagtgaa atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg
     5041 cccatgcaac agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag
     5101 ttctttaggc ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa
     5161 tagaccagtt gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg
     5221 ggtttgttac tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt
     5281 ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt
     5341 caaacaggag ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa
     5401 cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc
     5461 tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa
     5521 catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa
     5581 atgaaaaata tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa
     5641 aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact
     5701 atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat
     5761 cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg
     5821 gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa
     5881 cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca
     5941 ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat
     6001 cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg
     6061 gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag
     6121 gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag
     6181 tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca
     6241 aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa
     6301 gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg
     6361 atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg
     6421 aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc
     6481 gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg
     6541 tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa
     6601 tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga
     6661 aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac
     6721 aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca
     6781 aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataaattcgc tgagcgagga
     6841 tgcacatgtg accgagggaa gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta
     6901 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     6961 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     7021 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     7081 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     7141 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
     7201 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
     7261 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
     7321 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
     7381 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
     7441 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
     7501 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
     7561 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
     7621 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
     7681 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
     7741 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
     7801 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
     7861 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     7921 tcatctgatg ctggatgaat ttttctaata attaattgga ccgcggtccg cgcgttgtcc
     7981 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     8041 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     8101 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     8161 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     8221 ccgtaggccg gccgatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     8281 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     8341 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     8401 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     8461 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcctttaagg
     8521 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     8581 caaagcaatt ttgagtgaca caggaacact taacggctga catggggcgc gcccagctgt
     8641 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     8701 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcct
//
