LOCUS       sequence-403473-        8686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Tetracyline selectable marker and tagBFP 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC097
FEATURES             Location/Qualifiers
     source          1..8686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(689..694,695..711,712..717)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             765..1955
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1117..1136)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    complement(2035..2082)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2110..2115,2116..2131,2132..2138)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2146..2162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      2903..2997
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(3146..3151,3152..3169,3170..3176)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3230..3889
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3296..3315)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4231..4536
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4415..4434
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4690..4855
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4898..5343
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5344..5430,5431..6765)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6848..6942
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(7063..7082)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7074..7889
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            8056..8164
                     /note="incP origin of transfer"
     rep_origin      8175..8563
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8594..8680)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgaagaat tctcatgttt gacagcttat catcgataag ctttaatgcg
      721 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
      781 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
      841 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
      901 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
      961 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1021 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1081 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1141 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1201 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1261 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1321 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1381 cgcggggcat gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag
     1441 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1501 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1561 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1621 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     1681 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     1741 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1801 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1861 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1921 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     1981 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg gcttgaagtt
     2041 cctatacttt ctagagaata ggaacttcgg aataggtact tccgctacta gaattcgagc
     2101 ggggaattct tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
     2161 aattttcaca caggaaacag ctaaatgtct gaattgatta aagagaatat gcatatgaag
     2221 ttgtatatgg aaggtactgt tgataaccac cattttaaat gtacatctga gggagaaggt
     2281 aagccttacg aaggtactca aacaatgcgt attaaagtag ttgagggagg tcctttacca
     2341 ttcgcttttg acatattggc aacttcattc ttatatggat ctaagacatt tattaaccat
     2401 actcaaggta taccagattt ctttaaacaa tcatttcctg aaggattcac atgggagaga
     2461 gtaacaactt atgaagatgg tggagttttg actgctactc aggatacatc tttacaagac
     2521 ggttgtttga tttataatgt taagattcgt ggagtaaact tcacttctaa tggtccagtt
     2581 atgcagaaga aaacattggg atgggaggca ttcactgaga cattataccc tgctgatggt
     2641 ggattggaag gtcgtaacga catggcattg aaattagtag gaggttcaca cttgatagct
     2701 aatattaaga ctacatatcg ttctaagaaa ccagctaaaa acttgaaaat gcctggtgtt
     2761 tactatgttg attatcgttt agaaagaatt aaggaagcaa ataacgaaac ttacgttgag
     2821 caacatgaag tagctgttgc acgttattgt gacttgcctt ctaagttagg acacaaattg
     2881 aacgcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     2941 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatctc
     3001 ccgctttacg aattcccatg gggagcatgc ggagttacgt catccccacc ttccgctgcg
     3061 ttcttcatcg atgcccgctt agtgatatgc ttaaaactcc aagcacagcc ttctccccgg
     3121 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     3181 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     3241 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     3301 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3361 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3421 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     3481 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     3541 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     3601 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     3661 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     3721 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     3781 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     3841 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc
     3901 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     3961 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     4021 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     4081 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     4141 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     4201 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     4261 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccggacga
     4321 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     4381 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     4441 gtgccggtat ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     4501 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     4561 ccccggggat gctggttgtc attgcccctc cttcctctaa gtgataaggt tacttcctct
     4621 aaatgaccaa gcagggtatc taatcctgtt aacgctcgct cagagaattc gcatgcggag
     4681 tcgagggaga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     4741 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     4801 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacacgctg
     4861 agcgaattcc acgcttgtgg aattcctcga gggagttcac atatacctgc cgttcactat
     4921 tatttagtga aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat
     4981 gcccatgcaa cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta
     5041 gttctttagg cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa
     5101 atagaccagt tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc
     5161 gggtttgtta ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt
     5221 tggcgtcacc ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct
     5281 tcaaacagga gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga
     5341 acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg
     5401 ctggcaggag gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa
     5461 acatacggca tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa
     5521 aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat ctcttctgca
     5581 aaaggcctgg acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac
     5641 tatcacggct accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca
     5701 tcgatttaca tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct
     5761 ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa
     5821 acacaagaat ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac
     5881 actgatttct ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta
     5941 tcagcatcag acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac
     6001 ggtgacggaa aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca
     6061 ggcgacaacc atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta
     6121 gtatttgaag caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac
     6181 aaagcatact atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa
     6241 agcgataaaa aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac
     6301 gatgattaca cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat
     6361 gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc
     6421 cgcggatcaa aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat
     6481 gtttctaatt ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa
     6541 atggatcttg atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg
     6601 aaaggaaaca atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa
     6661 caatcaacgt ttgcgccaag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc
     6721 aaagacagca tccttgaaca aggacaatta acagttaaca aataaattcg ctgagcgagg
     6781 atgcacatgt gaccgaggga agcttgcggc cgcgtcgtga ctgggaaaac cctggcgact
     6841 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     6901 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
     6961 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     7021 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     7081 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
     7141 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
     7201 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
     7261 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
     7321 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
     7381 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
     7441 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
     7501 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
     7561 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
     7621 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
     7681 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
     7741 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     7801 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     7861 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc gcgcgttgtc
     7921 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     7981 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     8041 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     8101 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     8161 gccgtaggcc ggccgatctg aagatcagca gttcaacctg ttgatagtac gtactaagct
     8221 ctcatgtttc acgtactaag ctctcatgtt taacgtacta agctctcatg tttaacgaac
     8281 taaaccctca tggctaacgt actaagctct catggctaac gtactaagct ctcatgtttc
     8341 acgtactaag ctctcatgtt tgaacaataa aattaatata aatcagcaac ttaaatagcc
     8401 tctaaggttt taagttttat aagaaaaaaa agaatatata aggcttttaa agcctttaag
     8461 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
     8521 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatggggcg cgcccagctg
     8581 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     8641 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
