LOCUS       sequence-403477-        3225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of pyr4 gene from T. reesei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB271
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1162..1219
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1243..1260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1414..2002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1494..1513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2185..2279)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2300..2959)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2874..2893
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3121..3139
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3143..3186)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3189..3210
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggaggatctg tgtcttttgc cttgccttgt cttgtctggg ttcctgctcg tctgcggcaa
       61 tcggaacgcc gccagtgcgg tgccaagcaa accagccaag gtaggtacct accactaggc
      121 ttcttttctc gttgtctcac tctctctttt cctctttgtc ctctcttatc cccatctttt
      181 ctctctctct ctgctccttt cctaaccact tccctacctt tctctttttc cttttcttgt
      241 catctccatc ttggctgacg aaaaaggtct gactgggtag gtattatctg gcagacttgt
      301 gtgtatcatt caccctattt ctgcttcata gtacatgtac tgtacctgaa cggctcaacc
      361 gctatttacg actcttattt ttttgtggcg ttggtcacgt ttgccagctg ttgtccgtct
      421 ttctagggct cctcaaactt gacctgaccg agctcccttt ctggacccgg tgggcttcac
      481 ttccagctgc tgagcgacct gagccgaaca tcctcagtcc ttgtccagcg caattcattt
      541 tctttccttt tctttttttt tattcctttc tttactttta ttctctcttt ttctcctctt
      601 cctcttcttc ttctttctcc tcctcctcca tatcctcact ctcgtttccc tcattactac
      661 cctctcggct cctcaggtcc accaaccctc ccgcacccaa acctctgccg ctgaaaccca
      721 ttcggtggtc gccgtttttt tttttttttt tttctcaccc ccaaagtcgc aatatcgggt
      781 atcgccgccg gcattgaatc gccttctccg ctagcatcga ctactgctgc tctgctctcg
      841 ttgccagcgc tgctccctag aattttgacc aggggacgag cccgacatta aagcaactcc
      901 ctcgcctcga gatgactcgg atcgcacgaa attctcccaa tcgccgacag ttcctactcc
      961 tcttcctccc gcacggctgt cgcgcttcca acgtcattcg cacagcagaa ttgtgccatc
     1021 tctctctttt ttttcccccc ctctaaaccg ccacaacggc accctaaggg ttaaactatc
     1081 caaccagccg cagcctcagc ctctctcagc ctcatcagcc aatgtgagac cactcatcgc
     1141 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     1201 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     1261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1381 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     1441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2161 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     2221 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     2281 aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt
     2341 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     2401 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     2461 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     2521 ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     2581 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     2641 tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     2701 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     2761 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     2821 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2881 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2941 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     3001 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     3061 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3121 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     3181 gatttctgga attcgcggcc gcttctagag cgatgagtgg tctcg
//
