LOCUS       sequence-403487-       10563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_AspRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             194..988
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(248..267)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     858..877
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1028..1280
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1373..1397
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1918..2709
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2955..3543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3444..3463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3697..3714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3729..3869
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3795..3814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3955..3977
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4213..4407
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4473..5546)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5975..6604)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7935..7959
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8652..8996
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8940..8960
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9096..9120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9427..9451)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9459..9461,9462..9464,9465..10178)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9459..9461,9462..9464,9465..10175)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9504..9525)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9765..9784)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10112..10133
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10544..10563,1..160)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ccactgagcc gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat
       61 tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg
      121 ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca
      181 actcgatcga ggcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
      241 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
      301 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
      361 cctgaatgaa ctccaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
      421 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
      481 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
      541 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
      601 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
      661 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
      721 gcggatgccc gacggcgagg atctcgtcgt gacccacggc gatgcctgct tgccgaatat
      781 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
      841 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
      901 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
      961 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcggact ctagctagag
     1021 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     1081 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     1141 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     1201 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     1261 gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg gcgttaattc
     1321 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     1381 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     1441 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     1501 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     1561 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     1621 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1681 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1741 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1801 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1861 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1921 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1981 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2041 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2101 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2161 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2221 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2281 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2341 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2401 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2461 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2521 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2581 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2641 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2701 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     2761 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     2821 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     2881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3721 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3781 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3841 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3901 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3961 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     4021 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     4081 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     4141 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     4201 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     4261 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     4321 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     4381 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     4441 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     4501 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     4561 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     4621 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     4681 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     4741 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     4801 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     4861 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     4921 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     4981 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     5041 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     5101 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     5161 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     5221 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     5281 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     5341 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     5401 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     5461 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     5521 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     5581 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     5641 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     5701 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     5761 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     5821 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
     5881 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
     5941 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
     6001 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
     6061 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
     6121 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
     6181 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
     6241 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
     6301 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
     6361 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
     6421 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
     6481 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
     6541 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
     6601 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
     6661 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
     6721 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
     6781 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
     6841 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
     6901 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
     6961 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
     7021 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
     7081 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
     7141 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
     7201 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
     7261 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
     7321 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
     7381 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
     7441 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
     7501 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
     7561 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
     7621 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
     7681 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
     7741 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttaaagtact
     7801 ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     7861 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
     7921 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga
     7981 aggcgggaaa cgacaatctg atccaagctc aagctaagct tgagctctcc catatggtcg
     8041 actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt tctctatctc
     8101 tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac caccgataat
     8161 gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
     8221 tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga gatcaaatac
     8281 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
     8341 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
     8401 cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt tcctactgaa
     8461 tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc cgtgaagact
     8521 ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat cttcgtcaac
     8581 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     8641 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     8701 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
     8761 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga cagtggtccc
     8821 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     8881 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     8941 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggactccg
     9001 gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca atttactatt
     9061 ctagtcgacc tgcaggcggc cgcactagtg atatcacaag tttgtacaaa aaagcaggct
     9121 ccgcggccgc ccccttcacc atgtttattg gaccatcttg gccaaatact tctccacatg
     9181 ttggagctgc tagatttctt aattttctta tgtggtggtg gagaagaaga tggtgtctta
     9241 gagctattca actttttgct aagcaacaag gagcttctgc tacttcttct gctcaaagat
     9301 tttcttcttc ttctgcttat acttctgttt ctatgccacc agaatctgaa gttagatctt
     9361 ctgaaactgt tccacttgct ggaattggag atgcttgtat ggttatttct aagggtgggc
     9421 gcgccgaccc agctttcttg tacaaagtgg tgatatcaat ggtgagcaag ggcgaggagc
     9481 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     9541 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     9601 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     9661 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     9721 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     9781 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9841 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     9901 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     9961 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
    10021 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
    10081 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    10141 ccgggatcac tctcggcatg gacgagctgt acaagtaacc gcggccatgc tagagtccgc
    10201 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
    10261 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
    10321 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
    10381 attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct ctagaggatc
    10441 cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc
    10501 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
    10561 gag
//
