LOCUS       sequence-403488-       10503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_TrpRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..792
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1038..1626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1527..1546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1780..1797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1812..1952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1878..1897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2038..2060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2296..2490
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2556..3629)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4058..4687)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6018..6042
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        6735..7079
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7023..7043
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7179..7203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7450..7474)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7482..7484,7485..7487,7488..8201)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7482..7484,7485..7487,7488..8198)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7527..7548)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7788..7807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8135..8156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8567..8746
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8780..9574
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8834..8853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9444..9463
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9614..9866
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9959..9983
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
       61 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      121 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      181 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
      241 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      301 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
      361 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      421 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      481 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
      541 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
      601 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
      661 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
      721 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
      781 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
      841 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
      901 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
      961 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     1201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1801 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1861 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     1921 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     1981 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2041 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
     2101 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     2161 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     2221 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     2281 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     2341 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     2401 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     2461 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     2521 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     2581 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     2641 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     2701 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     2761 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     2821 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     2881 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     2941 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     3001 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     3061 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     3121 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     3181 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     3241 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     3301 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     3361 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     3421 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     3481 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     3541 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     3601 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     3661 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     3721 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     3781 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     3841 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     3901 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     3961 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     4021 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     4081 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     4141 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     4201 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     4261 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     4321 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
     4381 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
     4441 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     4501 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     4561 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     4621 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     4681 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     4741 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     4801 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     4861 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
     4921 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
     4981 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
     5041 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
     5101 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
     5161 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
     5221 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
     5281 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
     5341 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
     5401 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
     5461 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
     5521 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
     5581 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
     5641 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
     5701 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
     5761 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
     5821 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt
     5881 actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
     5941 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
     6001 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
     6061 tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc tcccatatgg
     6121 tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga ctttctctat
     6181 ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt caccaccgat
     6241 aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat ggttagagag
     6301 gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca ggagatcaaa
     6361 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     6421 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     6481 ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt agttcctact
     6541 gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact cgccgtgaag
     6601 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
     6661 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     6721 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     6781 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     6841 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc cgacagtggt
     6901 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     6961 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     7021 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggact
     7081 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
     7141 attctagtcg acctgcaggc ggccgcacta gtgatatcac aagtttgtac aaaaaagcag
     7201 gctccgcggc cgcccccttc accatgtgtg gaaagcttta tgctactgga aagctttgtt
     7261 gttgtggaag aggagatctt ggagcttggt ctgcttctgt tttttctaga ccaagatttt
     7321 tttcttctat gcaaggacca gctactatgg cttctctttc tattctttct gatggatcta
     7381 cttctcaaaa tatggttgtt aatccatgga atgtttctgc taagggagct ggaaagggtg
     7441 ggcgcgccga cccagctttc ttgtacaaag tggtgatatc aatggtgagc aagggcgagg
     7501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7681 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7921 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     7981 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     8041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     8101 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     8161 ccgccgggat cactctcggc atggacgagc tgtacaagta accgcggcca tgctagagtc
     8221 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     8281 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     8341 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     8401 ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgggc cctctagagg
     8461 atccccgggt accgcgaatt atcgatcatg agcggagaat taagggagtc acgttatgac
     8521 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
     8581 aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
     8641 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
     8701 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
     8761 ctctcaactc gatcgaggca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     8821 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     8881 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     8941 cggtgccctg aatgaactcc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     9001 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     9061 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     9121 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     9181 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     9241 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     9301 caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc
     9361 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     9421 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     9481 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     9541 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cggactctag
     9601 ctagagtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     9661 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     9721 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     9781 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     9841 cgcggtgtca tctatgttac tagatcgacc ggcatgcaag ctgataattc aattcggcgt
     9901 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     9961 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
    10021 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
    10081 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
    10141 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
    10201 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
    10261 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
    10321 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
    10381 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
    10441 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
    10501 atc
//
