LOCUS       sequence-403490-       10569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_ThrRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             193..987
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(247..266)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     857..876
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1027..1279
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1372..1396
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1917..2708
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2954..3542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3443..3462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3696..3713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3728..3868
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3794..3813)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3954..3976
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4212..4406
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4472..5545)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5974..6603)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7934..7958
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8651..8995
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8939..8959
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9095..9119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9432..9456)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9464..9466,9467..9469,9470..10183)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9464..9466,9467..9469,9470..10180)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9509..9530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9770..9789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10117..10138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10549..10569,1..159)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cactgagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt
       61 gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc
      121 tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa
      181 ctcgatcgag gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
      241 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
      301 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
      361 ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
      421 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
      481 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
      541 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
      601 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
      661 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
      721 cggatgcccg acggcgagga tctcgtcgtg acccacggcg atgcctgctt gccgaatatc
      781 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
      841 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
      901 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
      961 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcggactc tagctagagt
     1021 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1081 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     1141 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     1201 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     1261 tcatctatgt tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca
     1321 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     1381 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     1441 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     1501 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     1561 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     1621 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1681 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1741 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1801 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1861 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     1921 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1981 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2041 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2101 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2161 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2221 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2281 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2341 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2401 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2461 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2521 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2581 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2641 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2701 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     2761 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     2821 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     2881 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3061 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3601 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3661 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3721 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3841 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3901 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3961 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     4021 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     4081 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     4141 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     4201 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     4261 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     4321 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     4381 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     4441 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     4501 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     4561 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     4621 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     4681 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     4741 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     4801 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     4861 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     4921 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     4981 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     5041 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     5101 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     5161 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     5221 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     5281 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     5341 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
     5401 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
     5461 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
     5521 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
     5581 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
     5641 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
     5701 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
     5761 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
     5821 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
     5881 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
     5941 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
     6001 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
     6061 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
     6121 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
     6181 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
     6241 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
     6301 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
     6361 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
     6421 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
     6481 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
     6541 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
     6601 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
     6661 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
     6721 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
     6781 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
     6841 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
     6901 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
     6961 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
     7021 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
     7081 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
     7141 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
     7201 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
     7261 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
     7321 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
     7381 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
     7441 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
     7501 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
     7561 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
     7621 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
     7681 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
     7741 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt
     7801 taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
     7861 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     7921 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     7981 ggcgggaaac gacaatctga tccaagctca agctaagctt gagctctccc atatggtcga
     8041 ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct
     8101 acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg
     8161 agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     8221 acgcggcagg tctcatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc
     8281 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     8341 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     8401 ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat
     8461 caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg
     8521 gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     8581 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     8641 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     8701 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     8761 gccatcattg cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca
     8821 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     8881 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     8941 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg
     9001 tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc
     9061 tagtcgacct gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctc
     9121 cgcggccgcc cccttcacca tggttgatga aatggatgct cttcattcta atggaacttg
     9181 ggatcttgtt gttcttccat ctggaaagtc tactgttgga tgtagatggg tttatgctat
     9241 taaggttgga ccagatggac aagttgatag acttaaggct agacttgttg ctaagggata
     9301 tactcaaatt tatggatctg attatgctga tactttttct ccaattgcta agatttcttc
     9361 tgttagactt cttctttcta tggctgctat gtattcttgg ccactttatc aacttaaggg
     9421 tgggcgcgcc gacccagctt tcttgtacaa agtggtgata tcaatggtga gcaagggcga
     9481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     9661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     9901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     9961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
    10021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
    10081 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    10141 cgccgccggg atcactctcg gcatggacga gctgtacaag taaccgcggc catgctagag
    10201 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
    10261 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
    10321 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    10381 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg gccctctaga
    10441 ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag tcacgttatg
    10501 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
    10561 tgaaggagc
//
