LOCUS       sequence-403491-        9848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. cerevisiae expression vector for NlovFz2 reprogrammed guide 
            (targeting PSP1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, 
            Desimone A, Macrae RK, Zhang F
  TITLE     Fanzor is a eukaryotic programmable RNA-guided endonuclease.
  JOURNAL   Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
  PUBMED    37380027
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2023 Jun 28. doi: 10.1038/s41586-023-06356-2."
COMMENT     Sequence Label: pMJ127: pRS426-His-MBP-NlovFz2_PSP1
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3666..3690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3821..3938
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4119..4142
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             4260..4289
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4299..5399
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5373..5396
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5454..5474
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(7792..7816)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7864..7883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7865..7883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7893..7910)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7893..7909)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7902..7924)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8050..8505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8137..8156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8347..8368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8636..9439)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9440..9655)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9679..9698)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9798..9820
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ccaccgcggt ggcggccccc gtcccaccat cgggcgcgga
     3661 tcatcacaag tttgtacaaa aaagcaggct ctttggccat ataggccacc aggtcctgca
     3721 ggcgcgctct tcaggaggcg cgccaactgc cctcttaatt aacgaagaag atcggaccgg
     3781 ggccgcggat ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc
     3841 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     3901 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     3961 tagcttttat ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg
     4021 aacgaatcaa attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct
     4081 gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc
     4141 tgtaaatcta tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt
     4201 ttaaaacacc aagaacttag tttcgaataa acacacataa ataaaccatg aaatcttctc
     4261 accatcacca tcaccatcac catcaccatg gttcttctat gaaaatcgaa gaaggtaaac
     4321 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
     4381 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
     4441 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
     4501 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
     4561 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
     4621 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
     4681 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
     4741 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
     4801 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
     4861 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
     4921 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     4981 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     5041 tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta
     5101 acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg
     5161 atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt
     5221 acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag
     5281 gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg
     5341 tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta
     5401 attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc gaggaaaacc
     5461 tgtacttcca atccaatgca aacctactca tcctcctact aatccatctc ttgcacacgg
     5521 catcattcca ttctgggatg agtactcaca acaagtatcg gataaacttt gggcatgttc
     5581 aagagattcc tttcatgagt ttaatcagta caacaacaag ggatgcactg acggatggtt
     5641 caacttttct caattcactg tgattgaatc ccagccagta tttgatgttc cactcaatgt
     5701 tcatcactct ataacagaga atgtggcttt tgataactct aaaaagccac ctcagctcaa
     5761 gaaggcaaag aagggtcaga agactcccca aaaatttcaa gcggacaaat caatgaaaat
     5821 tcgtctctac cccaatgaac aagaaaggac aacattgaat cagtggatgg gaactgctcg
     5881 ttggatctac aacaagtgtt tagaattcac caacaaatcc aaaggtgtca agaaaaataa
     5941 gaagaatttc agaacgtttg tggtgaacaa cgataattat cagacagaaa atcagtgggt
     6001 tgtaaatact ccttatgacg taagagatgc tgccgctatt gaattgttaa ctgcgttcaa
     6061 cactaatttt gaaaagaaga aggctggaac gattgacaag ttcatgatcc gtttcaggcg
     6121 aaagaaagac agaaaagatc atttcgttct gcgttgcaag cactggaaga aaaagagtgg
     6181 aatgtattct tttataagga acatcaagag cgccgaacct ttgcctgagg agcttcagta
     6241 tgattccatc attattaaga acaagttgaa ccattattat ttatgtattc cacaagtact
     6301 ggatattagg ggtgagaacc aagcccctca acattcagga caagttgtag cattggatcc
     6361 aggagtaaga acatttcaga ccacctttga tttgaatggt tattcaacca aatggggctc
     6421 aggaggtgcc gaaagaattg gtagattgtg ttgcgcgtat gacaagttac aatcgaagtg
     6481 gtctcaacca gaagtcagac attgcaaaag atacaagtac aagagagcag gaagaagaat
     6541 tcaacaaaag attagaaata ttgtggatga tttacacaag aaactgtgtt tgtggctgtg
     6601 tcgaaactac caagtgatct tacttccttc gtttgagact cagaaaatgg taaagaaact
     6661 ccacaggaga attaatagca agacagcaag aaaaatgttg acttggtcac attacagatt
     6721 caagcaaaga ttgcttcaca aagctagaga gcatccgtgg acacacattt atattgtcaa
     6781 cgaagcgtat acatccaaaa cttgctcatg ttgtggacat gtttatacgg ttggatcatc
     6841 ggaagtgttt cgttgtccat catgtggctc aatttttgac agagatatca atggagccag
     6901 aaatattctt cttcgattcc ttactactca tagaattagt ttttgagcac cttgtgtgtt
     6961 gggtcttccc caccttgtgt gcgttgggtc ctttcccctg gctttcactc tttgagtgtt
     7021 tgcttgcaag atcagcactt tgttcgattt gtttgttttg ttcaaattgt tgatttaaat
     7081 aaaggcggag aagtcattta ataaggccac tgttaaaaat tgatgcatga acccaaagac
     7141 aataaatttg gcaataaaaa aaaattctga aggcggttgc ccgtgttgag tgtgccacat
     7201 agaaaacacc cgagcctcat tctcatcatc cgagagccaa attccttttt gaatttgaat
     7261 catcattgtt gaatttttat acaagagttt catctcaaaa acattgctgt ccccgagatt
     7321 ttaaaaagat catcatcatc agaaaattca tatgggagga atttgctcaa ccatgtccac
     7381 cagtacaaca ttaccagtcc agaaatttag acctattgtc atttgtggtc ctagcggagt
     7441 tggaaaagga agcttaagtc gactttgttc ccactgtact tttagctcgt acaaaataca
     7501 atatactttt catttctccg taaacaacat gttttcccat gtaatatcct tttctatttt
     7561 tcgttccgtt accaacttta cacatacttt atatagctat tcacttctat acactaaaaa
     7621 actaagacaa ttttaatttt gctgcctgcc atatttcaat ttgttataaa ttcctataat
     7681 ttatcctatt agtagctaaa aaaagatgaa tgtgaatcga atcctaagag aattcccctc
     7741 gaagatcgat ctggcggccc ggccggccgc ggccgcactc gagatatcta gacccagctt
     7801 tcttgtacaa agtggtgata gcttgtcgag aagtactcga gggggggccc ggtacccaat
     7861 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7921 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7981 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8041 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8101 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8161 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8221 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8281 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8341 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8401 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8461 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     8521 tctccttacg catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa
     8581 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     8641 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     8701 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     8761 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     8821 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     8881 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     8941 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     9001 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     9061 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     9121 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     9181 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     9241 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     9301 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     9361 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     9421 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
     9481 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     9541 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     9601 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt
     9661 ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     9721 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9781 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9841 acgcgcga
//
