LOCUS       sequence-403492-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_CytC_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             196..990
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(250..269)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     860..879
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1030..1282
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1375..1399
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1920..2711
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2957..3545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3446..3465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3699..3716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3731..3871
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3797..3816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3957..3979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4215..4409
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4475..5548)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5977..6606)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7937..7961
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8654..8998
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8942..8962
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9098..9122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9363..9387)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9395..9397,9398..9400,9401..10114)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9395..9397,9398..9400,9401..10111)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9440..9461)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9701..9720)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10048..10069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10480..10497,1..162)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 agccactgag ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt
       61 attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa
      121 tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca tattcactct
      181 caactcgatc gaggcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
      241 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
      301 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
      361 gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
      421 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
      481 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
      541 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
      601 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
      661 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
      721 gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg gcgatgcctg cttgccgaat
      781 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
      841 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
      901 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
      961 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgga ctctagctag
     1021 agtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     1081 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     1141 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     1201 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     1261 gtgtcatcta tgttactaga tcgaccggca tgcaagctga taattcaatt cggcgttaat
     1321 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     1381 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     1441 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     1501 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     1561 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     1621 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     1681 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1741 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     1801 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1861 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1921 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1981 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     2041 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2101 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2161 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2221 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2281 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2341 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2401 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2461 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2521 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2581 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2641 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     2701 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     2761 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     2821 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     2881 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3721 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     3841 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     3901 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     3961 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     4021 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     4081 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     4141 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     4201 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     4261 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     4321 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     4381 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     4441 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     4501 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     4561 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     4621 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     4681 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     4741 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     4801 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     4861 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     4921 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     4981 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     5041 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     5101 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     5161 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     5221 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     5281 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     5341 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     5401 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     5461 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     5521 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     5581 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     5641 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     5701 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     5761 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     5821 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     5881 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     5941 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     6001 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     6061 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     6121 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     6181 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     6241 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     6301 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     6361 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     6421 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     6481 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     6541 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     6601 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     6661 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     6721 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     6781 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
     6841 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     6901 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     6961 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     7021 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     7081 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     7141 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     7201 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     7261 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     7321 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     7381 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     7441 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     7501 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     7561 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     7621 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     7681 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
     7741 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta
     7801 ctttaaagta ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
     7861 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
     7921 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
     7981 gaaggcggga aacgacaatc tgatccaagc tcaagctaag cttgagctct cccatatggt
     8041 cgactagagc caagctgatc tcctttgccc cggagatcac catggacgac tttctctatc
     8101 tctacgatct aggaagaaag ttcgacggag aaggtgacga taccatgttc accaccgata
     8161 atgagaagat tagcctcttc aatttcagaa agaatgctga cccacagatg gttagagagg
     8221 cctacgcggc aggtctcatc aagacgatct acccgagtaa taatctccag gagatcaaat
     8281 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     8341 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     8401 tgcttcataa accaaggcaa gtaatagaga ttggagtctc taagaaagta gttcctactg
     8461 aatcaaaggc catggagtca aaaattcaga tcgaggatct aacagaactc gccgtgaaga
     8521 ctggcgaaca gttcatacag agtcttttac gactcaatga caagaagaaa atcttcgtca
     8581 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     8641 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     8701 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     8761 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcccctgcc gacagtggtc
     8821 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     8881 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     8941 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggactc
     9001 cggtattttt acaacaatta ccacaacaaa acaaacaaca aacaacatta caatttacta
     9061 ttctagtcga cctgcaggcg gccgcactag tgatatcaca agtttgtaca aaaaagcagg
     9121 ctccgcggcc gcccccttca ccatgcttaa ggctgctgga agaagatttt ctcttattgc
     9181 ttggagagga tcttctattt cttcttttac ttctgcttct cattgttata ttcattctat
     9241 tcatggaaat gctgctgctg ctggagttca tagaccagat actctttcta ttgctggagg
     9301 acttattaga ggattttctg ctggagctct tgatagatct catgaaaagg gtgggcgcgc
     9361 cgacccagct ttcttgtaca aagtggtgat atcaatggtg agcaagggcg aggagctgtt
     9421 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     9481 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     9541 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     9601 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     9661 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     9721 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     9781 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     9841 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     9901 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     9961 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
    10021 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
    10081 gatcactctc ggcatggacg agctgtacaa gtaaccgcgg ccatgctaga gtccgcaaaa
    10141 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
    10201 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
    10261 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
    10321 ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg ggccctctag aggatccccg
    10381 ggtaccgcga attatcgatc atgagcggag aattaaggga gtcacgttat gacccccgcc
    10441 gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaagg
//
