LOCUS       sequence-403493-       10443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Silene_conica _TyrRS_MitoTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             199..993
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(253..272)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     863..882
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1033..1285
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1378..1402
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1923..2714
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2960..3548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3449..3468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3702..3719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3734..3874
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3800..3819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3960..3982
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4218..4412
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4478..5551)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5980..6609)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7940..7964
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8657..9001
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8945..8965
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9101..9125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9312..9336)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9344..9346,9347..9349,9350..10063)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9344..9346,9347..9349,9350..10060)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9389..9410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     9997..10018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10429..10443,1..165)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aggagccact gagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
       61 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
      121 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
      181 tctcaactcg atcgaggcat gattgaacaa gatggattgc acgcaggttc tccggccgct
      241 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
      301 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
      361 ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc cacgacgggc
      421 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
      481 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
      541 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
      601 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
      661 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
      721 aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg
      781 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
      841 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
      901 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
      961 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc ggactctagc
     1021 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     1081 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     1141 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     1201 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     1261 gcggtgtcat ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt
     1321 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     1381 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     1441 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     1501 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     1561 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     1621 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     1681 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1741 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1801 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1861 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1921 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1981 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2041 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2101 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2161 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2221 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2281 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2341 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2401 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2461 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2521 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2581 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2641 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2701 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     2761 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     2821 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     2881 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3001 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3061 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3721 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3781 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3841 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3901 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3961 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     4021 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     4081 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     4141 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     4201 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     4261 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     4321 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     4381 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     4441 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     4501 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     4561 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     4621 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     4681 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     4741 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     4801 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     4861 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     4921 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     4981 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     5041 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     5101 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     5161 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     5221 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     5281 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     5341 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     5401 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     5461 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     5521 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     5581 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     5641 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     5701 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     5761 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     5821 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     5881 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     5941 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     6001 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     6061 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     6121 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     6181 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     6241 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     6301 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     6361 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     6421 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
     6481 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
     6541 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
     6601 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
     6661 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
     6721 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
     6781 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
     6841 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
     6901 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
     6961 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
     7021 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
     7081 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
     7141 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
     7201 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
     7261 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
     7321 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
     7381 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
     7441 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
     7501 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
     7561 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
     7621 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
     7681 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
     7741 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttaaa
     7801 gtactttaaa gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     7861 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     7921 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
     7981 actgaaggcg ggaaacgaca atctgatcca agctcaagct aagcttgagc tctcccatat
     8041 ggtcgactag agccaagctg atctcctttg ccccggagat caccatggac gactttctct
     8101 atctctacga tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg
     8161 ataatgagaa gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag
     8221 aggcctacgc ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca
     8281 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
     8341 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
     8401 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta
     8461 ctgaatcaaa ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga
     8521 agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg
     8581 tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     8641 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     8701 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     8761 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcccct gccgacagtg
     8821 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     8881 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     8941 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     9001 ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta
     9061 ctattctagt cgacctgcag gcggccgcac tagtgatatc acaagtttgt acaaaaaagc
     9121 aggctccgcg gccgccccct tcaccatgtc aaaactcatc aaacatttct ccaaattctc
     9181 aatcgtacac tactctaaac aatccccaat ccctcaaatt cagtacaatt cgtcttgttt
     9241 caatcaaggt aatataagga tgatgacgac ggcattagag gagagattta aaatgaaggg
     9301 tgggcgcgcc gacccagctt tcttgtacaa agtggtgata tcaatggtga gcaagggcga
     9361 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9421 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9481 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     9541 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9601 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9661 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9721 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     9781 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     9841 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     9901 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     9961 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    10021 cgccgccggg atcactctcg gcatggacga gctgtacaag taaccgcggc catgctagag
    10081 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
    10141 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
    10201 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    10261 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg gccctctaga
    10321 ggatccccgg gtaccgcgaa ttatcgatca tgagcggaga attaagggag tcacgttatg
    10381 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
    10441 tga
//
