LOCUS       sequence-403494-       10311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. cerevisiae expression vector for MmeFz2 native guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, 
            Desimone A, Macrae RK, Zhang F
  TITLE     Fanzor is a eukaryotic programmable RNA-guided endonuclease.
  JOURNAL   Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
  PUBMED    37380027
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2023 Jun 28. doi: 10.1038/s41586-023-06356-2."
COMMENT     Sequence Label: pMJ240: pRS426-His-MBP-MmeFz2_Native
FEATURES             Location/Qualifiers
     source          1..10311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3666..3690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3821..3938
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4119..4142
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             4260..4289
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4299..5399
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5373..5396
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5454..5474
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(8255..8279)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8327..8346)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8328..8346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8356..8373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8356..8372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8513..8968
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8600..8619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8810..8831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9099..9902)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9903..10118)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10142..10161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10261..10283
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ccaccgcggt ggcggccccc gtcccaccat cgggcgcgga
     3661 tcatcacaag tttgtacaaa aaagcaggct ctttggccat ataggccacc aggtcctgca
     3721 ggcgcgctct tcaggaggcg cgccaactgc cctcttaatt aacgaagaag atcggaccgg
     3781 ggccgcggat ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc
     3841 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     3901 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     3961 tagcttttat ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg
     4021 aacgaatcaa attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct
     4081 gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc
     4141 tgtaaatcta tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt
     4201 ttaaaacacc aagaacttag tttcgaataa acacacataa ataaaccatg aaatcttctc
     4261 accatcacca tcaccatcac catcaccatg gttcttctat gaaaatcgaa gaaggtaaac
     4321 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
     4381 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
     4441 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
     4501 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
     4561 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
     4621 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
     4681 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
     4741 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
     4801 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
     4861 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
     4921 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     4981 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     5041 tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta
     5101 acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg
     5161 atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt
     5221 acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag
     5281 gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg
     5341 tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta
     5401 attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc gaggaaaacc
     5461 tgtacttcca atccaatgca aagagaaaac gagaacagat gactttgtgg aaagccgcgt
     5521 ttgtcaacgg gcaggaaacg ttcaagtcct ggattgacaa ggcacgaatg ctggaattga
     5581 actgtgacgt ctcttctgca agctcgacac attactctga tctgaacttg aagacaaagt
     5641 gtgccaagac agacgacaaa tttatgtgta attattctgt ctgcatacga ccgacttcta
     5701 agcagaagcg aacgttgaac cagatgctca aggtgagcaa ctatgcttac aattggtgca
     5761 attacctagt taaggagaaa gacttcaaac cgaagcagtt cgacttgcaa agaattgtcg
     5821 ctaaaacgaa ttcaacagac gttccggccg aatacaggct tccaggagac gactggtttt
     5881 tcgacaacaa gatgtcctct atcaagttga ccgcctgtaa gaacttttgc accatgtaca
     5941 agtctactca aaccaaccag aagaaaacaa aggtcgatct cagaaacaaa gacattgttc
     6001 agttacgcga aggatcgttt gaagttcaaa gcaagtacgt gagattgcta acggaaaaag
     6061 acataccagg cgaacgaatt cggcaatcga gaattgcttt gatgccagac agtttttcga
     6121 aatcaaagaa ggactggaaa gaaaggtttc tcaggctttc gaaaaatgta tctaagatac
     6181 cacctttgag tcacgacatg aaggtatgca aacgtccgaa tgggaaattc atactgcaga
     6241 tttcatgcga tccaatatgt actcgacaga tacaagtgca aacttcagat tcgatatgtt
     6301 caatcgatcc gggaggcagg acttttgcga catgctatga tcctagcaat ataaagacat
     6361 ttcagatcgg acccgaggca gacaaaaagg aaatcattca cgaatttcac aacaagatcg
     6421 attatgttca ccgtctcttg tcgcatgctc aggagaaaaa gcagacgcaa gcggtgcaag
     6481 acagaatcgg acaactgaag aagctgcacc tcaaactgaa aacgtacgtc gacgacgtgc
     6541 acttgaaatt gtgctcgtac ctggtcaaga actacaaact ggtcgttctc ggcaagatat
     6601 cggtttcgtc catcgtgaga aaagacaggc cgaaccatct tgccaaaagc gctaacaggg
     6661 acttgctctg ttggcagcac tacagattca gacagagatt gttacacaga gtccgtggca
     6721 cggactgcga agtgatcata caagacgaac gttacacttc caagacctgt ggtaattgcg
     6781 gtgagaagaa caacaaactc ggtggaaagg aaacgtttac ttgtgaaagt tgcaattaca
     6841 aaactcatcg agacgtcaac ggagcgagaa acattctgtg caaatacttg ggactttttc
     6901 catttgcagc gtaacgaaag aaaaatgaca attaattgtt cgggttcgat tctatcccca
     6961 gggctcgaat gcatttttgt cacagatttt gccaatgcaa gatctggggg caagaatgtc
     7021 tccgggtgaa aagagtcagg ctggaaagat tacggggaca ggtgctcaaa cgcctttcca
     7081 ttttcggtat atataaaaac acaatgcttt gacatttctc atgtatcatc gttttgaatt
     7141 acaagaggtt tctgccgtat tggacaaaat catacaaagc cagtgatgcc atcatacatt
     7201 agctttccta tttaaaaaaa ctcgaatgtg gacagtgttg gcaaatttgt ccaggtatac
     7261 acaaatgcaa tgttttgaaa ttccttatac tatcatcaca ctgtcatagg agaagtttct
     7321 ccctacattg gacgctgaag tagaatgact ttgctatcag acaagtagta aaaaaatgtt
     7381 caacaatatg taatgcatta ctaatacagg agaaaattcc aacataatgt tgtttgtttt
     7441 ccttctgatc gaaatttgga aaaagttagc agcaggatag tgagaaaatg ataactatcc
     7501 tgtgacgcat cgggacaaaa ttccaacatg tagtacagta tgagatcggt aacatggata
     7561 gacattcgat gcccttcccc gtacatctaa ctgcggcgtc cggacagtta ggcaaccaaa
     7621 gagtgtattc gcacatctca ttagaggaac gtcgtgcttc cctttggcgg ggcaagagca
     7681 tttaatccct gtccatgaat cctcgcagcg tcgggttaag caattcccta taccggcttc
     7741 gctgcctcgc caaatataga gtcgggtcgt tacaaccagc attacactta acccatgccg
     7801 atcagaaatc caaacaagag aggaaaaaat gctcaacacc atgtaaggac taggaaaaaa
     7861 ttccaacatc atactattgc atcgaaaagc atttcaaatc tctgagtaac gtttgcttaa
     7921 gtcgactttg ttcccactgt acttttagct cgtacaaaat acaatatact tttcatttct
     7981 ccgtaaacaa catgttttcc catgtaatat ccttttctat ttttcgttcc gttaccaact
     8041 ttacacatac tttatatagc tattcacttc tatacactaa aaaactaaga caattttaat
     8101 tttgctgcct gccatatttc aatttgttat aaattcctat aatttatcct attagtagct
     8161 aaaaaaagat gaatgtgaat cgaatcctaa gagaattccc ctcgaagatc gatctggcgg
     8221 cccggccggc cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg
     8281 atagcttgtc gagaagtact cgaggggggg cccggtaccc aattcgccct atagtgagtc
     8341 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8401 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8461 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     8521 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8581 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8641 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8701 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8761 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8881 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8941 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     9001 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
     9061 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     9121 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     9181 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     9241 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     9301 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     9361 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     9421 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     9481 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     9541 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     9601 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     9661 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     9721 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     9781 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     9841 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     9901 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     9961 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
    10021 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
    10081 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca
    10141 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    10201 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    10261 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
