LOCUS       sequence-403496-        8468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the LTB4 sensor GEM-LTB4 along with the plasma membrane 
            targeted mKate2 tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Tamas SX, Roux BT, Vamosi B, Dehne FG, Torok A, Fazekas L, Enyedi B
  TITLE     A genetically encoded sensor for visualizing leukotriene B4 
            gradients in vivo.
  JOURNAL   Nat Commun. 2023 Aug 1;14(1):4610. doi: 10.1038/s41467-023-40326-6.
  PUBMED    37528073
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-1"; volume: "14(1):4610. doi"; pages: " 
            10.1038/s41467-023-40326-6"
COMMENT     Sequence Label: pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4
FEATURES             Location/Qualifiers
     source          1..8468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        640..658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             789..1484
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             1503..1559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     sig_peptide     1560..1619
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2507..2528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2634..2655)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2895..2914)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3509..3526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3515..3739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3746..4103
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3746..3937
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3769..3789)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3954..4089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4131..4730
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4131..4150)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4627..4647
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4744..4763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    4773..4854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     4798..4817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5329..5349)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5329..5347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5368..5384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5368..5384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5381..5403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5392..5408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5416..5422,5423..5440,5441..5446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5461..5482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5599..5616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5770..6358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5850..5869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6529..7320,7321..7389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7152..7171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7390..7494)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7521..7976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7608..7627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7818..7839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8102..8124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8116..8133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8117..8133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8143..8161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agaagcttgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      601 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      661 gacccaagct ggctagcgct accggactca gatctgccac catgggctgt gtttgcagct
      721 caaaccctga aaacaacaac aacgatccca cccgttctgc gtcaggtgct ggtgctatcc
      781 tatccaccat ggtgtctgag ctgattaagg agaacatgca catgaagctg tatatggagg
      841 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
      901 gcacccagac catgagaatc aaggccgtcg agggcggccc tctccccttc gccttcgaca
      961 tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc
     1021 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
     1081 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
     1141 acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
     1201 cactcggctg ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca
     1261 gagccgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca
     1321 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca
     1381 gaagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg
     1441 ctgtggccag atactgcgac ctccctagca aactggggca cagactgtac aagggatccg
     1501 gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac cccggtccta
     1561 tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt tccactggtg
     1621 acaccagcct gtataagaag gtgggaacca ccggtaacac tacatcttct gccgcacccc
     1681 cctcactagg tgtagagttc atctctctgc tggctatcat cctgctgtca gtggcgctgg
     1741 ctgtggggct tcccggcaac agctttgtgg tgtggagtat cctgaaaagg atgcagaagc
     1801 gctctgtcac tgccctgatg gtgctgaacc tggccctggc cgacctggcc gtattgctca
     1861 ctgctccctt tttccttcac ttcctggccc aaggcacctg gagttttgga ctggctggtt
     1921 gccgcctgtg tcactatgtc tgcggagtca gcatgtacgc cagcgtcctg cttatcacgg
     1981 ccatgagtct agaccgctca ctggcggtgg cccgcccctt tgtgtcccag aagctacgca
     2041 ccaaggcgat ggcccggcgg gtgctggcag gcatctgggt gttgtccttt ctgctggcca
     2101 cacccgtcct cgcgtaccgc acagtagtgc cctggaaaac gaacatgagc ctgtgcttcc
     2161 cgcggtaccc cagcgaaggg caccgggcct tccatctaat cttcgaggct gtcacgggct
     2221 tcctgctgcc cttcctggct gtggtggcca gctactcgga catagggcgt cggctgcagg
     2281 cccggcgcag cagcctgatt aacgtctata tcaaggccga caagcagaag aacggcatca
     2341 aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact
     2401 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2461 gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2521 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg
     2581 gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2641 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg
     2701 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2761 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2821 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca
     2881 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2941 acaccctggt gaaccgcatc gagcttaagg gcatcgactt caaggaggac ggcaacatcc
     3001 tggggcacaa gctggagtac aacaaccacg atcagttccg ccgcagtcga cgcaccggcc
     3061 gcctggtggt gctcatcatc ctgaccttcg ccgccttctg gctgccctac cacgtggtga
     3121 acctggctga ggcgggccgc gcgctggccg gccaggccgc cgggttaggg ctcgtgggga
     3181 agcggctgag cctggcccgc aacgtgctca tcgcactcgc cttcctgagc agcagcgtga
     3241 accccgtgct gtacgcgtgc gccggcggcg gcctgctgcg ctcggcgggc gtgggcttcg
     3301 tcgccaagct gctggagggc acgggctccg aggcgtccag cacgcgccgc gggggcagcc
     3361 tgggccagac cgctaggagc ggccccgccg ctctggagcc cggcccttcc gagagcctca
     3421 ctgcctccag ccctctcaag ttaaacgagc tcaactaaga attcgcggcc gctcgagtct
     3481 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3721 gatgcggtgg gctctatgga ccggactgag gcggaaagaa ccagctgtgg aatgtgtgtc
     3781 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3841 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3901 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3961 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4021 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4081 ttggaggcct aggcttttgc aaaaagctag attttctgac cctagccacc atgaccgagt
     4141 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4201 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4261 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4321 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4381 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4441 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4501 tcctggccac cgtcggcgtg tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4561 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4621 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4681 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ggatctcgag
     4741 ctcgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4801 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     4861 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     4921 aagtaaaacc tctacaaatg tggtacttaa gagggatcga taaggatcta gcttgtggaa
     4981 ggctactcga aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa
     5041 ttgagtgtat gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat
     5101 gaatcattct ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct
     5161 aactgaccta agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga
     5221 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct
     5281 ctagctagag tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     5341 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5401 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5461 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5521 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5581 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5641 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5701 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5761 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5821 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5881 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5941 ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6001 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6061 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6961 cggtcctccg atcgttgtca gaagtaactt ggccgcagtg ttatcactca tggttatggc
     7021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7501 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggagtggt
     7561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7861 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7921 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     7981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8041 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8101 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     8161 ggcgaattgg agctcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
     8221 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
     8281 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
     8341 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
     8401 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
     8461 atggcttt
//
