LOCUS       sequence-403499-        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman
            SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, 
            Allain V, Li Z, Marson A, Eyquem J, Wilson RC
  TITLE     Peptide-mediated delivery of CRISPR enzymes for the efficient 
            editing of primary human lymphocytes.
  JOURNAL   Nat Biomed Eng. 2023 May;7(5):647-660. doi: 
            10.1038/s41551-023-01032-2. Epub 2023 Apr 25.
  PUBMED    37147433
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-023-01032-2"; journalName: "Nat Biomed Eng"; date: 
            "2023-05"; volume: "7"; issue: "5"; pages: "647-660"
COMMENT     Sequence Label: pRM055 His-TEV-Cas9 6xNLS 0C
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2665..2684
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2768..2787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2875..2897
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2917..2934
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2935..2955
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2959..2979
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2989..3009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3019..3039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3049..3069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3079..3099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3127..7230
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7237..7257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7264..7284
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7409..7427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7423..7470
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(7836..7841,7842..7858,7859..7864))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7891..7909)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7977..8081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8082..8150,8151..8942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8300..8319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc
     2821 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
     2881 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatgaaaac
     2941 ctgtacttcc aatccaatga aaacctgtac ttccaatcca atgccactcc caagaagaag
     3001 cgaaaggtgg gtgggtcccc aaagaagaaa cggaaagtag gcggctcccc caaaaagaag
     3061 cgaaaagtag ggggtagccc caagaagaag cggaaggtag gtatccacgg agtcccagca
     3121 gctaccatgg ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg
     3181 gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca
     3241 gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca
     3301 gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt
     3361 attagttatc tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt
     3421 catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt
     3481 tttggaaata tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg
     3541 cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta
     3601 gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat
     3661 agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa
     3721 aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa
     3781 tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt
     3841 gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg
     3901 gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta
     3961 ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat
     4021 gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca
     4081 gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta
     4141 gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga
     4201 tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca
     4261 attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg
     4321 ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag
     4381 ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag
     4441 aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc
     4501 aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt
     4561 gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt
     4621 gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt
     4681 acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca
     4741 tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa
     4801 gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaaagttt tgatagtgtt
     4861 gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta
     4921 aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat
     4981 attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca
     5041 tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt
     5101 tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca
     5161 atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat
     5221 gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat
     5281 agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta
     5341 cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat
     5401 atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga
     5461 gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag
     5521 catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat
     5581 ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc
     5641 gatcacattg ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg
     5701 cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag
     5761 atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat
     5821 aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa
     5881 cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc
     5941 atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta
     6001 aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt
     6061 aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt
     6121 aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt
     6181 cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt
     6241 tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc
     6301 aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga
     6361 gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca
     6421 gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag
     6481 cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg
     6541 gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa
     6601 tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg
     6661 attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta
     6721 cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga
     6781 gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta
     6841 gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt
     6901 gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga attttctaag
     6961 cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga
     7021 gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt
     7081 ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct
     7141 acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca
     7201 cgcattgatt tgagtcagct aggaggtgac gggtcaccta agaaaaaacg aaaagttgag
     7261 gatcctaaaa agaaacgaaa agtttaaatt ggaagtggat aacggatccg cgatcgcggc
     7321 gcgccacctg gtggccggcc ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc
     7381 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     7441 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
     7501 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     7561 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     7621 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     7681 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     7741 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     7801 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     7861 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7921 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8041 aaccctgata aatgcttcaa taacattgaa aaaggaagag tatgagtatt caacatttcc
     8101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8281 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     8341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8461 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     8641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8941 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9001 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
