LOCUS       sequence-403500-       10491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Silene_conica _PheRS_PlastidTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..1087)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(1516..2145)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3476..3500
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4193..4537
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4481..4501
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4637..4661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4896..4920)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4928..4930,4931..4933,4934..5647)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4928..4930,4931..4933,4934..5644)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4973..4994)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5234..5253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5581..5602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6013..6192
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6226..7020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6280..6299)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6890..6909
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7060..7312
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7405..7429
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7950..8741
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8987..9575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9476..9495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9729..9746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9761..9901
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9827..9846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9987..10009
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10245..10439
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
       61 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
      121 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
      181 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
      241 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
      301 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
      361 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
      421 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
      481 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
      541 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
      601 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
      661 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
      721 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
      781 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
      841 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
      901 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
      961 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
     1021 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
     1081 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
     1141 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
     1201 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
     1261 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
     1321 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
     1381 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
     1441 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
     1501 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
     1561 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
     1621 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
     1681 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
     1741 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
     1801 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
     1861 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
     1921 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
     1981 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
     2041 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
     2101 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
     2161 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
     2221 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
     2281 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
     2341 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
     2401 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
     2461 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
     2521 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
     2581 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
     2641 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
     2701 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
     2761 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
     2821 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
     2881 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
     2941 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
     3001 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
     3061 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
     3121 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
     3181 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
     3241 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
     3301 tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac tttaaagtac
     3361 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
     3421 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
     3481 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
     3541 gatccaagct caagctaagc ttgagctctc ccatatggtc gactagagcc aagctgatct
     3601 cctttgcccc ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt
     3661 tcgacggaga aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca
     3721 atttcagaaa gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctcatca
     3781 agacgatcta cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag
     3841 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca
     3901 agatcagaag tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag
     3961 taatagagat tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa
     4021 aaattcagat cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga
     4081 gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
     4141 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     4201 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     4261 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     4321 gaaaggctat cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca
     4381 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     4441 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     4501 cctctatata aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac
     4561 cacaacaaaa caaacaacaa acaacattac aatttactat tctagtcgac ctgcaggcgg
     4621 ccgcactagt gatatcacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
     4681 catggctaca atctcctttg ctcaatccac aatcttctct aaacattcac atataatctg
     4741 cagaaatcat gttggattct tcaatttatc tcgcgctttt tccacatctt cttcagccct
     4801 ttcctctaac aatgttcgcc ctaaaaaatg gagacgacat gttgttgctt caatggtgga
     4861 ggttggtggg gtcaaaatta agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     4921 gatatcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4981 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5041 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5101 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5161 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5221 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5281 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5341 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5401 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5461 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5521 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5581 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5641 caagtaaccg cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
     5701 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     5761 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     5821 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag
     5881 gcatgcgacg tcgggccctc tagaggatcc ccgggtaccg cgaattatcg atcatgagcg
     5941 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
     6001 ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt ctggagttta
     6061 atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac
     6121 tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc
     6181 ggtatccaat tagagtctca tattcactct caactcgatc gaggcatgat tgaacaagat
     6241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
     6421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6721 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
     6781 gtgacccacg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7021 gcgggactct ggggttcgga ctctagctag agtcaagcag atcgttcaaa catttggcaa
     7081 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     7141 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     7201 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     7261 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca
     7321 tgcaagctga taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     7381 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     7441 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     7501 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     7561 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     7621 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     7681 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     7741 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     7801 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     7861 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     7921 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     7981 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     8041 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     8101 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     8161 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     8221 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     8281 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     8341 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     8401 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     8461 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     8521 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     8581 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     8641 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     8701 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     8761 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     8821 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     8881 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     8941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9121 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     9781 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     9841 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     9901 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     9961 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
    10021 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
    10081 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
    10141 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
    10201 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
    10261 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
    10321 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
    10381 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
    10441 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc a
//
