LOCUS       sequence-403502-        8468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with
            the plasma membrane targeted mKate2 tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Tamas SX, Roux BT, Vamosi B, Dehne FG, Torok A, Fazekas L, Enyedi B
  TITLE     A genetically encoded sensor for visualizing leukotriene B4 
            gradients in vivo.
  JOURNAL   Nat Commun. 2023 Aug 1;14(1):4610. doi: 10.1038/s41467-023-40326-6.
  PUBMED    37528073
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-1"; volume: "14(1):4610. doi"; pages: " 
            10.1038/s41467-023-40326-6"
COMMENT     Sequence Label: pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut
FEATURES             Location/Qualifiers
     source          1..8468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        23..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        651..669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             800..1495
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             1514..1570
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     sig_peptide     1571..1630
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2518..2539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2645..2666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2906..2925)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3520..3537)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3526..3750
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3757..4114
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3757..3948
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3780..3800)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3965..4100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4142..4741
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4142..4161)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4638..4658
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4755..4774)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    4784..4865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     4809..4828
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5340..5360)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5340..5358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5379..5395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5379..5395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5392..5414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5403..5419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5427..5433,5434..5451,5452..5457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5472..5493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5610..5627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5781..6369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5861..5880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6540..7331,7332..7400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7163..7182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7401..7505)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7532..7987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7619..7638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7829..7850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8113..8135
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8127..8144
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8128..8144
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8154..8172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcatggctt tagaagcttg ttgacattga ttattgacta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      661 cactataggg agacccaagc tggctagcgc taccggactc agatctgcca ccatgggctg
      721 tgtttgcagc tcaaaccctg aaaacaacaa caacgatccc acccgttctg cgtcaggtgc
      781 tggtgctatc ctatccacca tggtgtctga gctgattaag gagaacatgc acatgaagct
      841 gtatatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
      901 gccctacgag ggcacccaga ccatgagaat caaggccgtc gagggcggcc ctctcccctt
      961 cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac
     1021 ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt
     1081 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     1141 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
     1201 gcagaagaaa acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg
     1261 cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
     1321 cttgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
     1381 ctatgtggac agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca
     1441 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acagactgta
     1501 caagggatcc ggagccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa
     1561 ccccggtcct atggagacag acacactcct gctatgggta ctgctgctct gggttccagg
     1621 ttccactggt gacaccagcc tgtataagaa ggtgggaacc accggtaaca ctacatcttc
     1681 tgccgcaccc ccctcactag gtgtagagtt catctctctg ctggctatca tcctgctgtc
     1741 agtggcgctg gctgtggggc ttcccggcaa cagctttgtg gtgtggagta tcctgaaaag
     1801 gatgcagaag cgctctgtca ctgccctgat ggtgctgaac ctggccctgg ccgacctggc
     1861 cgtattgctc actgctccct ttttccttca cttcctggcc caaggcacct ggagttttgg
     1921 actggctggt tgccgcctgt gtcactatgt ctgcggagtc agcatgtacg ccagcgtcct
     1981 gcttatcacg gccatgagtc tagaccgctc actggcggtg gcccgcccct ttgtgtccca
     2041 gaagctacgc accaaggcga tggcccggcg ggtgctggca ggcatctggg tgttgtcctt
     2101 tctgctggcc acacccgtcc tcgcatatgc cacagtagtg ccctggaaaa cgaacatgag
     2161 cctgtgcttc ccgcggtacc ccagcgaagg gcaccgggcc ttccatctaa tcttcgaggc
     2221 tgtcacgggc ttcctgctgc ccttcctggc tgtggtggcc agctactcgg acatagggcg
     2281 tcggctgcag gcccggcgca gcagcctgat taacgtctat atcaaggccg acaagcagaa
     2341 gaacggcatc aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct
     2401 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2461 ccactacctg agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat
     2521 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2581 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2701 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagcttaag ggcatcgact tcaaggagga
     3001 cggcaacatc ctggggcaca agctggagta caacaaccac gatcagttcc gccgcagtcg
     3061 acgcaccggc cgcctggtgg tgctcatcat cctgaccttc gccgccttct ggctgcccta
     3121 ccacgtggtg aacctggctg aggcgggccg cgcgctggcc ggccaggccg ccgggttagg
     3181 gctcgtgggg aagcggctga gcctggcccg caacgtgctc atcgcactcg ccttcctgag
     3241 cagcagcgtg aaccccgtgc tgtacgcgtg cgccggcggc ggcctgctgc gctcggcggg
     3301 cgtgggcttc gtcgccaagc tgctggaggg cacgggctcc gaggcgtcca gcacgcgccg
     3361 cgggggcagc ctgggccaga ccgctaggag cggccccgcc gctctggagc ccggcccttc
     3421 cgagagcctc actgcctcca gccctctcaa gttaaacgag ctcaactaag aattcgcggc
     3481 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3541 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3601 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3661 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3721 ggcatgctgg ggatgcggtg ggctctatgg accggactga ggcggaaaga accagctgtg
     3781 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3841 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4021 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4081 aggaggcttt tttggaggcc taggcttttg caaaaagcta gattttctga ccctagccac
     4141 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     4201 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     4261 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4321 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4381 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4441 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4501 gcccgcgtgg ttcctggcca ccgtcggcgt gtcgcccgac caccagggca agggtctggg
     4561 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4621 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4681 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4741 aggatctcga gctcgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4801 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4861 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4921 ttttttaaag caagtaaaac ctctacaaat gtggtactta agagggatcg ataaggatct
     4981 agcttgtgga aggctactcg aaatgtttga cccaagttaa acaatttaaa ggcaatgcta
     5041 ccaaatacta attgagtgta tgtaaacttc tgacccactg ggaatgtgat gaaagaaata
     5101 aaagctgaaa tgaatcattc tctctactat tattctgata tttcacattc ttaaaataaa
     5161 gtggtgatcc taactgacct aagacaggga atttttacta ggattaaatg tcaggaattg
     5221 tgaaaaagtg agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactgt
     5281 atagggatcc tctagctaga gtcgacctcg agggggggcc cggtacccag cttttgttcc
     5341 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     5401 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5461 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5521 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5581 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5641 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5701 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5761 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5941 gcctttctcc cttcgggaac cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6181 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6601 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6661 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6721 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6781 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6841 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6901 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6961 gttagctcct tcggtcctcc gatcgttgtc agaagtaact tggccgcagt gttatcactc
     7021 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7081 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7141 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7201 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7261 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7321 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7381 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7441 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7501 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     7561 gcgggagtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7741 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7861 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7981 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8041 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8101 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     8161 actcactata gggcgaattg gagctcggat ccctatacag ttgaagtcgg aagtttacat
     8221 acacttaagt tggagtcatt aaaactcgtt tttcaactac tccacaaatt tcttgttaac
     8281 aaacaatagt tttggcaagt cagttaggac atctactttg tgcatgacac aagtcatttt
     8341 tccaacaatt gtttacagac agattatttc acttataatt cactgtatca caattccagt
     8401 gggtcagaag tttacataca ctaagttgac tgtgccttta aacagcttgg aaaattccag
     8461 aaaatgat
//
