LOCUS       sequence-403507-       10521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_AsnRS_TP_GFP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             202..996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(256..275)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     866..885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1036..1288
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1381..1405
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1926..2717
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2963..3551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3452..3471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3705..3722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3737..3877
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3803..3822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3963..3985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4221..4415
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4481..5554)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5983..6612)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7943..7967
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8660..9004
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8948..8968
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9104..9128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9393..9417)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9425..9427,9428..9430,9431..10144)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9425..9427,9428..9430,9431..10141)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9470..9491)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9731..9750)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10078..10099
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10510..10521,1..168)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
       61 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
      121 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
      181 cactctcaac tcgatcgagg catgattgaa caagatggat tgcacgcagg ttctccggcc
      241 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      301 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      361 tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg
      421 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      481 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      541 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      601 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      661 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      721 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccacggcga tgcctgcttg
      781 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      841 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      901 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
      961 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggactct
     1021 agctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     1081 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     1141 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     1201 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     1261 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc
     1321 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     1381 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     1441 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     1501 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     1561 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     1621 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     1681 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1741 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1801 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1861 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1921 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1981 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2041 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2101 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2161 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2221 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2281 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2341 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2401 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2461 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2521 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2581 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2641 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2701 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     2761 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     2821 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     2881 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3721 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3781 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3841 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3901 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3961 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     4021 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     4081 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     4141 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     4201 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     4261 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     4321 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     4381 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     4441 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     4501 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     4561 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     4621 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     4681 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     4741 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     4801 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     4861 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     4921 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     4981 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     5041 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     5101 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     5161 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     5221 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     5281 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     5341 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     5401 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     5461 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     5521 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     5581 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     5641 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     5701 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     5761 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     5821 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     5881 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     5941 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     6001 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     6061 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     6121 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
     6181 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
     6241 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
     6301 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
     6361 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
     6421 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
     6481 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
     6541 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
     6601 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
     6661 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
     6721 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
     6781 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
     6841 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
     6901 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
     6961 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
     7021 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
     7081 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
     7141 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
     7201 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
     7261 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
     7321 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
     7381 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
     7441 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
     7501 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
     7561 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
     7621 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
     7681 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
     7741 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
     7801 aaagtacttt aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
     7861 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
     7921 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
     7981 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg agctctccca
     8041 tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
     8101 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
     8161 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
     8221 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
     8281 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
     8341 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
     8401 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
     8461 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
     8521 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
     8581 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     8641 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     8701 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     8761 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
     8821 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     8881 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     8941 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     9001 ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat
     9061 ttactattct agtcgacctg caggcggccg cactagtgat atcacaagtt tgtacaaaaa
     9121 agcaggctcc gcggccgccc ccttcaccat gcttgattct aaggttcttc ttgctcttta
     9181 tcatcatctt ggacaactta ttcatcatgg aatggttaga gctgctattg gaaagtttaa
     9241 tgctcttgga cttattactt ctagatattt tccatctcca tctggaagaa gacaaccagc
     9301 tctttcttct cattgtcatc ttccaatttc tcttgctcca ccaagaatgg gagatcataa
     9361 gactggactt tctcaaaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggtgat
     9421 atcaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     9481 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     9541 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     9601 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     9661 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     9721 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     9781 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     9841 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     9901 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     9961 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
    10021 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
    10081 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
    10141 gtaaccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc
    10201 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt
    10261 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    10321 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca
    10381 tgcgacgtcg ggccctctag aggatccccg ggtaccgcga attatcgatc atgagcggag
    10441 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
    10501 gaactgacag aaccgcaacg t
//
