LOCUS       sequence-403508-       10395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Silene_conica _PheRS_MitoTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      974..1562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1463..1482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1716..1733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1748..1888
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1814..1833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1974..1996
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2232..2426
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2492..3565)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3994..4623)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5954..5978
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        6671..7015
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6959..6979
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7115..7139
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7278..7302)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7310..7312,7313..7315,7316..8029)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7310..7312,7313..7315,7316..8026)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7355..7376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7616..7635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7963..7984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8395..8574
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8608..9402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8662..8681)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9272..9291
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9442..9694
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9787..9811
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             join(10332..10395,1..728)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
       61 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      121 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      181 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
      241 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
      301 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
      361 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
      421 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
      481 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      541 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      601 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
      661 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
      721 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
      781 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
      841 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
      901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1081 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1621 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1681 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1741 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     1801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     1861 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     1921 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1981 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     2041 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     2101 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     2161 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     2221 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     2281 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     2341 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     2401 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     2461 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     2521 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     2581 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     2641 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     2701 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     2761 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     2821 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     2881 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     2941 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     3001 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     3061 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     3121 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     3181 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     3241 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     3301 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     3361 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
     3421 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
     3481 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
     3541 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
     3601 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
     3661 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
     3721 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
     3781 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
     3841 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
     3901 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
     3961 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
     4021 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
     4081 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
     4141 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
     4201 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
     4261 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
     4321 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
     4381 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
     4441 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
     4501 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
     4561 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
     4621 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
     4681 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
     4741 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
     4801 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
     4861 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
     4921 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
     4981 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
     5041 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
     5101 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
     5161 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
     5221 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
     5281 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
     5341 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
     5401 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
     5461 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
     5521 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
     5581 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
     5641 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
     5701 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
     5761 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt
     5821 taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
     5881 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     5941 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6001 ggcgggaaac gacaatctga tccaagctca agctaagctt gagctctccc atatggtcga
     6061 ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct
     6121 acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg
     6181 agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     6241 acgcggcagg tctcatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc
     6301 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     6361 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     6421 ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat
     6481 caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg
     6541 gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     6601 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     6661 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     6721 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     6781 gccatcattg cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca
     6841 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     6901 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     6961 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg
     7021 tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc
     7081 tagtcgacct gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctc
     7141 cgcggccgcc cccttcacca tggcagcaaa aaccattact cgctccactt tcatctccaa
     7201 aaatcggctc ttcagtttat atcgcgcttt ctccacttct cctgttacta ctcttccctc
     7261 taagggtggg cgcgccgacc cagctttctt gtacaaagtg gtgatatcaa tggtgagcaa
     7321 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     7381 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     7441 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     7501 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     7561 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     7621 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     7681 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     7741 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     7801 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     7861 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     7921 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     7981 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac cgcggccatg
     8041 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     8101 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     8161 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     8221 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgggccc
     8281 tctagaggat ccccgggtac cgcgaattat cgatcatgag cggagaatta agggagtcac
     8341 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     8401 caacgttgaa ggagccactg agccgcgggt ttctggagtt taatgagcta agcacatacg
     8461 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt
     8521 tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct
     8581 catattcact ctcaactcga tcgaggcatg attgaacaag atggattgca cgcaggttct
     8641 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     8701 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     8761 gacctgtccg gtgccctgaa tgaactccaa gacgaggcag cgcggctatc gtggctggcc
     8821 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     8881 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     8941 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     9001 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     9061 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     9121 gccaggctca aggcgcggat gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc
     9181 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     9241 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     9301 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     9361 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     9421 gactctagct agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg
     9481 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     9541 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     9601 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     9661 ttatcgcgcg cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa
     9721 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     9781 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     9841 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     9901 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     9961 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
    10021 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct
    10081 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
    10141 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
    10201 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
    10261 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
    10321 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
    10381 ttggcgtcat cgagc
//
