LOCUS       sequence-403509-       10470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Determine where transit peptide targets GFP in N. benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Warren JM, Broz AK, Martinez-Hottovy A, Elowsky C, Christensen AC, 
            Sloan DB
  TITLE     Rewiring of Aminoacyl-tRNA Synthetase Localization and Interactions 
            in Plants With Extensive Mitochondrial tRNA Gene Loss.
  JOURNAL   Mol Biol Evol. 2023 Jul 5;40(7):msad163. doi: 
            10.1093/molbev/msad163.
  PUBMED    37463427
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol."; date: "2023-07-5"; volume: "40(7):msad163. doi"; pages: " 
            10.1093/molbev/msad163"
COMMENT     Sequence Label: Silene_vulgaris _TyrRS_MitoTP_pK7FWG2
FEATURES             Location/Qualifiers
     source          1..10470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             189..983
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(243..262)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     853..872
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1023..1275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1368..1392
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1913..2704
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2950..3538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3439..3458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3692..3709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3724..3864
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3790..3809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3950..3972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4208..4402
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4468..5541)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5970..6599)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7930..7954
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8647..8991
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8935..8955
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9091..9115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9329..9353)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9361..9363,9364..9366,9367..10080)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9361..9363,9364..9366,9367..10077)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9406..9427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9667..9686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10014..10035
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10446..10470,1..155)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 gagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
       61 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
      121 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaactcg
      181 atcgaggcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
      241 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      301 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      361 atgaactcca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      421 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      481 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      541 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      601 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
      661 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga
      721 tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg
      781 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
      841 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
      901 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
      961 gccttcttga cgagttcttc tgagcgggac tctggggttc ggactctagc tagagtcaag
     1021 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1081 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1141 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1201 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1261 ctatgttact agatcgaccg gcatgcaagc tgataattca attcggcgtt aattcagtac
     1321 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     1381 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     1441 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     1501 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     1561 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     1621 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     1681 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1741 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     1801 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1861 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     1921 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1981 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     2041 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     2101 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     2161 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     2221 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     2281 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     2341 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     2401 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2461 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2521 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2581 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2641 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     2701 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     2761 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     2821 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     2881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3061 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3721 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     3781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     3841 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     3901 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3961 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     4021 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     4081 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     4141 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     4201 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     4261 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     4321 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     4381 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
     4441 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     4501 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     4561 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     4621 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     4681 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     4741 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     4801 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     4861 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     4921 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     4981 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     5041 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     5101 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     5161 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     5221 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     5281 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     5341 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     5401 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     5461 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     5521 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     5581 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     5641 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     5701 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     5761 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     5821 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     5881 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     5941 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     6001 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     6061 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     6121 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     6181 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     6241 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     6301 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     6361 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     6421 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     6481 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     6541 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     6601 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     6661 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     6721 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     6781 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
     6841 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
     6901 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
     6961 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
     7021 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
     7081 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
     7141 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
     7201 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
     7261 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
     7321 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
     7381 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
     7441 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
     7501 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
     7561 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
     7621 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
     7681 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
     7741 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa
     7801 gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
     7861 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
     7921 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg
     7981 ggaaacgaca atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag
     8041 agccaagctg atctcctttg ccccggagat caccatggac gactttctct atctctacga
     8101 tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa
     8161 gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc
     8221 ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca aataccttcc
     8281 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
     8341 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
     8401 taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa
     8461 ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga
     8521 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     8581 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     8641 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     8701 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     8761 tcattgcgat aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga
     8821 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     8881 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     8941 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt
     9001 tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt
     9061 cgacctgcag gcggccgcac tagtgatatc acaagtttgt acaaaaaagc aggctccgcg
     9121 gccgccccct tcaccatgag aaggtcaata agtcagatta ttcattttaa gaaaaccatt
     9181 tataaaaccc taaaccatgt ccaatcgcca aaaatagctc actcccaatt tatccctttc
     9241 gctcgtctct accattttcc ccacacacag atacaacagt tacaggcacc ttcaaccaca
     9301 acaacaatgg caaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggtgatatca
     9361 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     9421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     9481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     9541 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     9601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     9661 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     9721 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     9781 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     9841 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     9901 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     9961 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
    10021 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
    10081 ccgcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
    10141 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
    10201 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
    10261 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg
    10321 acgtcgggcc ctctagagga tccccgggta ccgcgaatta tcgatcatga gcggagaatt
    10381 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac
    10441 tgacagaacc gcaacgttga aggagccact
//
