LOCUS       sequence-403518-       10317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Koutelou E, Sato S, Tomomori-Sato C, Florens L, Swanson SK, Washburn
            MP, Kokkinaki M, Conaway RC, Conaway JW, Moschonas NK
  TITLE     Neuralized-like 1 (Neurl1) targeted to the plasma membrane by 
            N-myristoylation regulates the Notch ligand Jagged1.
  JOURNAL   J Biol Chem. 2008 Feb 15;283(7):3846-53. doi: 
            10.1074/jbc.M706974200. Epub 2007 Dec 12.
  PUBMED    18077452
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M706974200"; journalName: "J Biol Chem"; date: 
            "2008-02-15- 15"; volume: "283"; issue: "7"; pages: "3846-53"
COMMENT     Sequence Label: Jag1-HA pIRES
FEATURES             Location/Qualifiers
     source          1..10317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        800..1179
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1180..1391
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1342..1362
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1388..1412
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1552..1684
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1667..1686
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1729..1747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1826..2392
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1986..2003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2213..2232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2414..2430
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2414..2430
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             6516..6605
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    6683..6804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6694..6713)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6748..6767
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6990..7445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7077..7096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7287..7308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7562..7919
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7562..7753
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7585..7605)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7770..7905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7970..8764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8024..8043)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8634..8653
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8828..8876
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8917..8936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9036..9058
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9096..9114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9182..9286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9287..9355,9356..10147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9505..9524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
      661 cttcaatatt ggccattagc catattattc attggttata tagcataaat caatattggc
      721 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
      781 ccaatatgac cgccatgttg gcattgatta ttgactagtt attaatagta atcaattacg
      841 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      901 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      961 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1021 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     1081 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     1141 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1201 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1261 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1321 tgcgatcgcc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1381 taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc
     1441 acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca
     1501 gtgactctct taaggtagcc ttgcagaagt tggtcgtgag gcactgggca ggtaagtatc
     1561 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
     1621 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
     1681 acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta atacgactca
     1741 ctataggcta gcctcgagaa ttcacgcgtc gagcatgcat ctagggcggc caattccgcc
     1801 cctctccccc ccccccctct ccctctcccc ccccccccct aacgttactg gccgaagccg
     1861 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     1921 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     1981 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2041 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2101 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     2161 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     2221 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     2281 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     2341 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     2401 caacccggga tcctctagaa ctagtggatc ccccgggctg cagggttttt tttttttttt
     2461 tttcctcccc tccctccccc gcgtttcatg cagctgatcc gaaagggaat aaaaggctgc
     2521 gcataatcat aatataaaag aaggggagcg cgagagaagg aaagaaagcc gagaggtgga
     2581 agagggggga gcgcctcaaa gaagcgatca gaataataaa aggaggccgg gctctttgcc
     2641 ttctggaacg cgcggctctt gaaagggctt ttgaaaagta gtgttgtttt ccagtcgtgc
     2701 atgctccaat ccacggagta tattagagcc gggacgcggc ggtcgcgggg gcagcgacga
     2761 cggcagcctc ggagggagca ccagcgctag cagcggcggc ggcgtccgga gtgcccccgg
     2821 cgcgcggcgc agcgatgcgg tccccacgga cgcgcggccg gccggggcgc cccttgagcc
     2881 ttctgctcgc cctgctctgt gccctgcgag ccaaggtgtg cggggcctcg ggtcaatttg
     2941 agctggagat cctgtccatg cagaacgtga acggagagtt acagaatggg aactgttgcg
     3001 gcggagcccg gaaccctggc gaccgcaagt gcacccgcga cgagtgtgac acgtacttca
     3061 aagtgtgcct caaggagtat cagtcccgcg tcacggccgg gggaccctgc agcttcggct
     3121 cagggtctac gcctgtcatc gggggcaata ccttcaatct caaggccagc cgcggcaacg
     3181 accgtaatcg catcgtactg cctttcagtt tcgcctggcc gaggtcctac actttgctgg
     3241 tggaggcatg ggattccagt aatgacacta ttcaacctga tagcataatt gaaaaggctt
     3301 ctcactcagg catgataaac cccagccggc agtggcagac actgaaacag aacacaggga
     3361 ttgcccactt tgagtatcag atccgagtga cctgtgatga ccactactat ggctttggct
     3421 gcaacaagtt ctgtcggccc agagatgact tctttggaca ttatgcctgc gaccagaatg
     3481 gcaacaaaac ctgcatggaa ggctggatgg gtcctgagtg taacaaagca atatgccgac
     3541 agggctgcag tcccaagcat ggctcctgca aactcccagg tgactgcagg tgccagtatg
     3601 gttggcaggg cctgtactgt gataagtgca tcccgcaccc aggatgtgtc cacggcacct
     3661 gcaatgaacc ctggcagtgc ctctgtgaaa ccaactgggg tggacagctc tgtgacaaag
     3721 atctgaatta ctgtgggact catcagccct gtctcaaccg gggaacatgt agcaacactg
     3781 gccctgacaa ataccagtgt tcctgccccg agggctactc aggaccgaac tgtgaaattg
     3841 ctgagcatgc ctgcctctct gatccctgcc acaacagagg cagctgcaag gagacctcct
     3901 cgggctttga gtgtgagtgt tctccaggct ggactggacc cacgtgttcc acaaacatcg
     3961 atgactgttc tccaaataac tgttcccatg gaggcacctg ccaggatcta gtgaatggat
     4021 tcaagtgcgt gtgcccaccc cagtggactg gcaagacttg tcagttagat gcaaatgagt
     4081 gcgaggccaa gccttgtgta aatgccagat cctgtaagaa tctgattgcc agctactact
     4141 gtgattgtct tcctggctgg atgggtcaga attgtgacat aaatatcaat gactgccttg
     4201 gccagtgtca gaatgatgcc tcctgtcggg atttggttaa tggttatcgc tgtatctgtc
     4261 cacctggcta tgcgggcgat cactgtgaaa gagacatcga tgaatgtgct agcaacccct
     4321 gcttgaatgg gggtcactgt cagaatgaaa ttaacagatt ccagtgtctg tgtcccactg
     4381 gtttctctgg aaacctctgt cagctggaca tcgattactg cgagcccaac ccttgccaga
     4441 atggcgccca atgctacaat cgtgccagtg actatttctg caagtgccct gaggactacg
     4501 agggcaagaa ctgctcacac ctgaaagacc actgccgcac cactccctgt gaagtgattg
     4561 acagctgcac cgtggccatg gcctccaacg atactcctga aggggtgcgg tatatctctt
     4621 ccaacgtctg tggtccccat gggaagtgca agagcgagtc aggaggcaaa ttcacctgtg
     4681 actgtaacaa aggcttcacc ggcacctact gccatgaaaa tatcaacgac tgtgagggca
     4741 acccctgtac gaacggtggc acgtgcatcg acggcgttaa ctcctacaag tgtatctgta
     4801 gtgacggctg ggagggcgcg cactgtgaga acaacataaa tgactgtagc cagaaccctt
     4861 gccactatgg gggtacatgt cgagacctgg tcaatgactt ttactgtgac tgcaaaaatg
     4921 ggtggaaagg gaaaacctgc cactcgcgtg acagccagtg tgacgaagcc acgtgtaata
     4981 acggtggtac ctgctatgat gaagtggata cgtttaagtg catgtgtcct ggtggctggg
     5041 aaggaacaac ctgtaatata gctagaaaca gtagctgcct gccgaacccc tgtcataatg
     5101 gaggcacctg cgtggtcaat ggagactcct tcacctgtgt ctgcaaagaa ggctgggagg
     5161 ggccgatttg tactcaaaat accaacgact gcagtcctca cccttgttac aacagcggaa
     5221 cctgtgtgga tggagataac tggtaccggt gcgaatgtgc ccctggtttt gctgggccag
     5281 actgcaggat aaacatcaat gagtgtcagt cttccccttg tgcctttggg gccacctgtg
     5341 tggatgagat caatggctac cagtgtatct gccctccagg acatagtggt gccaagtgcc
     5401 atgaagtttc agggagatct tgcatcacca tggggagagt gatacttgat ggggccaagt
     5461 gggatgacga ctgtaacacc tgccagtgcc tgaatggacg ggtggcctgc tccaaggtct
     5521 ggtgtggccc gagaccttgc cggctccaca aagggcacgg cgagtgtccc aatgggcaga
     5581 gctgcatccc agtcctggat gaccagtgtt tcgtgcgccc ctgcactggt gctggcgagt
     5641 gtcggtcctc cagcctccag ccagtgaaga ccaagtgtac atcggactct tattaccagg
     5701 acaattgtgc gaacatcact ttcaccttta acaaggagat gatgtctcca ggtctcacca
     5761 ctgagcacat ttgcagcgaa ttgaggaatc tgaatattct gaagaatgtt tctgctgagt
     5821 attcaatcta catagcctgt gagccttcct tatcagcaaa caatgaaata cacgtggcca
     5881 tttctgccga agacatccgg gatgatggaa accctgtcaa ggaaattact gataaaataa
     5941 tagatctcgt tagtaaacgg gatgggaaca gctcacttat cgctgctgtt gcagaagtca
     6001 gagttcagag gcgccctctg aaaaacagaa cagatttcct ggttcctctg ctgagctctg
     6061 tcttaacagt cgcttgggtc tgttgcttgg tgacagcctt ctactggtgt gtgcggaagc
     6121 ggcggcggaa gcccagcagc cacacccact ctgcccctga ggacaacacc accaacaatg
     6181 tgcgcgaaca gctgaaccaa atcaaaaacc ccatcgagaa acacggagcc aacacagtcc
     6241 ccatcaagga ttatgagaac aaaaactcca aaatgtcaaa aatcaggaca cacaactcgg
     6301 aagtggaaga ggatgatatg gataagcacc agcagaaagt ccggtttgcc aaacagcccg
     6361 tgtacacgct ggtagacaga gaggagaagg cccccagcgg cacgccgaca aaacacccga
     6421 actggacaaa caaacaggac aacagagact tggaaagtgc ccagagcttg aaccggatgg
     6481 agtacatcgt aatccgcggt ggcggccgca tcttttaccc atacgatgtt cctgactatg
     6541 cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt
     6601 acgctgctca gtgcggccgc tctagagtcg acccgggcgg ccgcttccct ttagtgaggg
     6661 ttaatgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6721 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6781 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     6841 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     6901 cgataaggat cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6961 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     7021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7441 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc
     7501 ggatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
     7561 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7741 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7921 agcttgattc ttctgacaca acagtctcga acttaaggct agagccacca tgattgaaca
     7981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     8161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     8401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8461 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     8521 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8581 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8641 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8701 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8761 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8821 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     8881 gatagcgata aggatccgcg tatggtgcac tctcagtaca atctgctctg atgccgcata
     8941 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     9001 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     9061 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     9121 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     9181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9481 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9541 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9601 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9661 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9721 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9781 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9841 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9901 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9961 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    10021 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    10081 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10141 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
