LOCUS       sequence-403519-        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Tschaharganeh DF, Chen X, Latzko P, Malz M, Gaida MM, Felix K, Ladu 
            S, Singer S, Pinna F, Gretz N, Sticht C, Tomasi ML, Delogu S, Evert 
            M, Fan B, Ribback S, Jiang L, Brozzetti S, Bergmann F, Dombrowski F,
            Schirmacher P, Calvisi DF, Breuhahn K
  TITLE     Yes-Associated Protein Up-regulates Jagged-1 and Activates the NOTCH
            Pathway in Human Hepatocellular Carcinoma.
  JOURNAL   Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 
            10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.
  PUBMED    23419361
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.02.009"; journalName: "Gastroenterology"; 
            date: "2013-06"; volume: "144"; issue: "7"; pages: "1530-1542.e12"
COMMENT     Sequence Label: pT3-EFa1-HA-Jag1
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1417..1436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1489..1522
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1663..2841
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1894..2832
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2789..2809
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     2858..2877
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2858..2876
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2955..2979
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6706..6795
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(6822..6846)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6899..6940
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6978..6995)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6984..7208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7278..7311
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7367..7387
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(7769..7786)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7769..7785)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7778..7800)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7994..8013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8113..8135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8173..8191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8259..8363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8364..8432,8433..9224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8582..8601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
     1021 ggtcgactct agacctatac agttgaagtc ggaagtttac atacacttaa gttggagtca
     1081 ttaaaactcg tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa
     1141 gtcagttagg acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag
     1201 acagattatt tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata
     1261 cactaagttg actgtgcctt taaacagctt ggaaaattcc agaaaatgat gtcatggctt
     1321 tagaagcttc tgatagacta attgacatca tttgagtcaa ttggaggtgt acctgtggat
     1381 gtatttcaag gggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     1441 agaatgcagc ggccgctagc tcgagatctt aattaaacta gtatcgatat aacttcgtat
     1501 aatgtatgct atacgaagtt atatgtacgg gccagatata cgcgttgaca ttgattattg
     1561 actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
     1621 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     1681 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     1741 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     1801 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     1861 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     1921 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     1981 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     2041 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     2101 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     2161 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     2221 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     2281 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     2341 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     2401 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     2461 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     2521 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     2581 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     2641 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     2701 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     2761 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     2821 cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga
     2881 cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
     2941 ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg
     3001 actggatccg gtaccgaatt caccatgcgg tccccacgga cgcgcggccg gccggggcgc
     3061 cccttgagcc ttctgctcgc cctgctctgt gccctgcgag ccaaggtgtg cggggcctcg
     3121 ggtcaatttg agctggagat cctgtccatg cagaacgtga acggagagtt acagaatggg
     3181 aactgttgcg gcggagcccg gaaccctggc gaccgcaagt gcacccgcga cgagtgtgac
     3241 acgtacttca aagtgtgcct caaggagtat cagtcccgcg tcacggccgg gggaccctgc
     3301 agcttcggct cagggtctac gcctgtcatc gggggcaata ccttcaatct caaggccagc
     3361 cgcggcaacg accgtaatcg catcgtactg cctttcagtt tcgcctggcc gaggtcctac
     3421 actttgctgg tggaggcatg ggattccagt aatgacacta ttcaacctga tagcataatt
     3481 gaaaaggctt ctcactcagg catgataaac cccagccggc agtggcagac actgaaacag
     3541 aacacaggga ttgcccactt tgagtatcag atccgagtga cctgtgatga ccactactat
     3601 ggctttggct gcaacaagtt ctgtcggccc agagatgact tctttggaca ttatgcctgc
     3661 gaccagaatg gcaacaaaac ctgcatggaa ggctggatgg gtcctgagtg taacaaagca
     3721 atatgccgac agggctgcag tcccaagcat ggctcctgca aactcccagg tgactgcagg
     3781 tgccagtatg gttggcaggg cctgtactgt gataagtgca tcccgcaccc aggatgtgtc
     3841 cacggcacct gcaatgaacc ctggcagtgc ctctgtgaaa ccaactgggg tggacagctc
     3901 tgtgacaaag atctgaatta ctgtgggact catcagccct gtctcaaccg gggaacatgt
     3961 agcaacactg gccctgacaa ataccagtgt tcctgccccg agggctactc aggaccgaac
     4021 tgtgaaattg ctgagcatgc ctgcctctct gatccctgcc acaacagagg cagctgcaag
     4081 gagacctcct cgggctttga gtgtgagtgt tctccaggct ggactggacc cacgtgttcc
     4141 acaaacatcg atgactgttc tccaaataac tgttcccatg gaggcacctg ccaggatcta
     4201 gtgaatggat tcaagtgcgt gtgcccaccc cagtggactg gcaagacttg tcagttagat
     4261 gcaaatgagt gcgaggccaa gccttgtgta aatgccagat cctgtaagaa tctgattgcc
     4321 agctactact gtgattgtct tcctggctgg atgggtcaga attgtgacat aaatatcaat
     4381 gactgccttg gccagtgtca gaatgatgcc tcctgtcggg atttggttaa tggttatcgc
     4441 tgtatctgtc cacctggcta tgcgggcgat cactgtgaaa gagacatcga tgaatgtgct
     4501 agcaacccct gcttgaatgg gggtcactgt cagaatgaaa ttaacagatt ccagtgtctg
     4561 tgtcccactg gtttctctgg aaacctctgt cagctggaca tcgattactg cgagcccaac
     4621 ccttgccaga atggcgccca atgctacaat cgtgccagtg actatttctg caagtgccct
     4681 gaggactacg agggcaagaa ctgctcacac ctgaaagacc actgccgcac cactccctgt
     4741 gaagtgattg acagctgcac cgtggccatg gcctccaacg atactcctga aggggtgcgg
     4801 tatatctctt ccaacgtctg tggtccccat gggaagtgca agagcgagtc aggaggcaaa
     4861 ttcacctgtg actgtaacaa aggcttcacc ggcacctact gccatgaaaa tatcaacgac
     4921 tgtgagggca acccctgtac gaacggtggc acgtgcatcg acggcgttaa ctcctacaag
     4981 tgtatctgta gtgacggctg ggagggcgcg cactgtgaga acaacataaa tgactgtagc
     5041 cagaaccctt gccactatgg gggtacatgt cgagacctgg tcaatgactt ttactgtgac
     5101 tgcaaaaatg ggtggaaagg gaaaacctgc cactcgcgtg acagccagtg tgacgaagcc
     5161 acgtgtaata acggtggtac ctgctatgat gaagtggata cgtttaagtg catgtgtcct
     5221 ggtggctggg aaggaacaac ctgtaatata gctagaaaca gtagctgcct gccgaacccc
     5281 tgtcataatg gaggcacctg cgtggtcaat ggagactcct tcacctgtgt ctgcaaagaa
     5341 ggctgggagg ggccgatttg tactcaaaat accaacgact gcagtcctca cccttgttac
     5401 aacagcggaa cctgtgtgga tggagataac tggtaccggt gcgaatgtgc ccctggtttt
     5461 gctgggccag actgcaggat aaacatcaat gagtgtcagt cttccccttg tgcctttggg
     5521 gccacctgtg tggatgagat caatggctac cagtgtatct gccctccagg acatagtggt
     5581 gccaagtgcc atgaagtttc agggagatct tgcatcacca tggggagagt gatacttgat
     5641 ggggccaagt gggatgacga ctgtaacacc tgccagtgcc tgaatggacg ggtggcctgc
     5701 tccaaggtct ggtgtggccc gagaccttgc cggctccaca aagggcacgg cgagtgtccc
     5761 aatgggcaga gctgcatccc agtcctggat gaccagtgtt tcgtgcgccc ctgcactggt
     5821 gctggcgagt gtcggtcctc cagcctccag ccagtgaaga ccaagtgtac atcggactct
     5881 tattaccagg acaattgtgc gaacatcact ttcaccttta acaaggagat gatgtctcca
     5941 ggtctcacca ctgagcacat ttgcagcgaa ttgaggaatc tgaatattct gaagaatgtt
     6001 tctgctgagt attcaatcta catagcctgt gagccttcct tatcagcaaa caatgaaata
     6061 cacgtggcca tttctgccga agacatccgg gatgatggaa accctgtcaa ggaaattact
     6121 gataaaataa tagatctcgt tagtaaacgg gatgggaaca gctcacttat cgctgctgtt
     6181 gcagaagtca gagttcagag gcgccctctg aaaaacagaa cagatttcct ggttcctctg
     6241 ctgagctctg tcttaacagt cgcttgggtc tgttgcttgg tgacagcctt ctactggtgt
     6301 gtgcggaagc ggcggcggaa gcccagcagc cacacccact ctgcccctga ggacaacacc
     6361 accaacaatg tgcgcgaaca gctgaaccaa atcaaaaacc ccatcgagaa acacggagcc
     6421 aacacagtcc ccatcaagga ttatgagaac aaaaactcca aaatgtcaaa aatcaggaca
     6481 cacaactcgg aagtggaaga ggatgatatg gataagcacc agcagaaagt ccggtttgcc
     6541 aaacagcccg tgtacacgct ggtagacaga gaggagaagg cccccagcgg cacgccgaca
     6601 aaacacccga actggacaaa caaacaggac aacagagact tggaaagtgc ccagagcttg
     6661 aaccggatgg agtacatcgt aatccgcggt ggcggccgca tcttttaccc atacgatgtt
     6721 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
     6781 gttccagatt acgctgctca gtgctgactc gagatatcta gacccagctt tcttgtacaa
     6841 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     6901 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgagt
     6961 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7141 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7201 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7261 ctgtagcggc gcattaaata acttcgtata atgtatgcta tacgaagtta tatcgtatgc
     7321 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac
     7381 acattccaca gaattccttg aaatacatcc acaggtacac ctccaattga ctcaaatgat
     7441 gtcaattagt ctatcagaag cttctaaagc catgacatca ttttctggaa ttttccaagc
     7501 tgtttaaagg cacagtcaac ttagtgtatg taaacttctg acccactgga attgtgatac
     7561 agtgaattat aagtgaaata atctgtctgt aaacaattgt tggaaaaatg acttgtgtca
     7621 tgcacaaagt agatgtccta actgacttgc caaaactatt gtttgttaac aagaaatttg
     7681 tggagtagtt gaaaaacgag ttttaatgac tccaacttaa gtgtatgtaa acttccgact
     7741 tcaactgtag ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg
     7801 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7861 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7921 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     7981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     8041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8161 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9361 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
