LOCUS       sequence-403521-        6318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GCaMP7f in CaMKII expressing cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Cohen-Kashi Malina K, Tsivourakis E, Kushinsky D, Apelblat D, 
            Shtiglitz S, Zohar E, Sokoletsky M, Tasaka GI, Mizrahi A, Lampl I, 
            Spiegel I
  TITLE     NDNF interneurons in layer 1 gain-modulate whole cortical columns 
            according to an animal's behavioral state.
  JOURNAL   Neuron. 2021 Jul 7;109(13):2150-2164.e5. doi: 
            10.1016/j.neuron.2021.05.001. Epub 2021 May 25.
  PUBMED    34038743
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.05.001"; journalName: "Neuron"; date: 
            "2021-07-7- 7"; volume: "109"; issue: "13"; pages: "2150-2164.e5"
COMMENT     Sequence Label: pAAV-CaMKII-jGCaMP7f-WPRE
FEATURES             Location/Qualifiers
     source          1..6318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..1293
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1337..1346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1343..1351,1352..1369,1370..1372,1373..1405,1406..1408,
                     1409..1432,1433..1459,1460..1516,1517..1522,1523..1792,
                     1793..1810,1811..2245,2246..2251,2252..2695)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      1439..1448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1729..1750
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1856..1877)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2117..2136)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2706..3294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2759..2779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3177..3188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3297..3313)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3298..3314)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3343..3464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3354..3373)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3408..3427
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3843..4431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3923..3942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4602..5393,5394..5462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5225..5244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5463..5567)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5593..6048)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5730..5751)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5942..5961
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   6120..6249
                     /label=AAV2 ITR
ORIGIN
        1 ttaacattat ggccttaggt cacttcatct ccatggggtt cttcttctga ttttctagaa
       61 aatgagatgg gggtgcagag agcttcctca gtgacctgcc cagggtcaca tcagaaatgt
      121 cagagctaga acttgaactc agattactaa tcttaaattc catgccttgg gggcatgcaa
      181 gtacgatata cagaaggagt gaactcatta gggcagatga ccaatgagtt taggaaagaa
      241 gagtccaggg cagggtacat ctacaccacc cgcccagccc tgggtgagtc cagccacgtt
      301 cacctcatta tagttgcctc tctccagtcc taccttgacg ggaagcacaa gcagaaactg
      361 ggacaggagc cccaggagac caaatcttca tggtccctct gggaggatgg gtggggagag
      421 ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg tgacagatag gggtgagaaa
      481 gcagacagag tcattccgtc agcattctgg gtctgtttgg tacttcttct cacgctaagg
      541 tggcggtgtg atatgcacaa tggctaaaaa gcagggagag ctggaaagaa acaaggacag
      601 agacagaggc caagtcaacc agaccaattc ccagaggaag caaagaaacc attacagaga
      661 ctacaagggg gaagggaagg agagatgaat tagcttcccc tgtaaacctt agaacccagc
      721 tgttgccagg gcaacggggc aatacctgtc tcttcagagg agatgaagtt gccagggtaa
      781 ctacatcctg tctttctcaa ggaccatccc agaatgtggc acccactagc cgttaccata
      841 gcaactgcct ctttgcccca cttaatccca tcccgtctgt taaaagggcc ctatagttgg
      901 aggtggggga ggtaggaaga gcgatgatca cttgtggact aagtttgttc gcatcccctt
      961 ctccaacccc ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca
     1021 cagtcctgca gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa
     1081 ggcaggcagg tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg
     1141 gttgccatgg ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc
     1201 ctgctcagaa gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg
     1261 ggcaggcgag tggcccctag ttctgggggc agctctagag cggtaccgga cttcaagctg
     1321 ctagcaagga tccaccgcca ccatgggttc tcatcatcat catcatcatg gtatggctag
     1381 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc
     1441 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
     1501 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
     1561 caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
     1621 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1681 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1741 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     1801 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1861 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     1921 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1981 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2041 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     2101 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2161 cgacaccctg gtgaaccgca tcgagcttaa gggcatcgac ttcaaggagg acggcaacat
     2221 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
     2281 atttaaagag ctgttctccc tatttgacaa ggacggggat gggacaataa caaccaagga
     2341 gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat
     2401 gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat
     2461 gatggcaaga aaaatgaaat acacagacag tgaagaagaa attagagaag cgttccgtgt
     2521 gtttgataag gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa
     2581 ccttggagag aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga
     2641 tggggatggt caggtaaact acgaagagtt tgtacaaatg atgacagcga agtgagctta
     2701 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2761 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2821 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2881 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2941 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3001 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3061 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3121 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3181 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3241 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3301 accgtcgacc tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt
     3361 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3421 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3481 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     3541 tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag
     3601 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     3661 ctgcgcgctc gctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5581 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5641 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5701 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5761 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5821 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5881 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5941 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6001 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     6061 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6121 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg
     6181 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
     6241 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta
     6301 ggaagatctc tgcagagg
//
