LOCUS       sequence-403586-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid for engineering cells to 
            express human CD28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, 
            Trinidad M, Ngo W, Doudna JA
  TITLE     In vivo human T cell engineering with enveloped delivery vehicles.
  JOURNAL   Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z.
  PUBMED    38212493
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z."
COMMENT     Sequence Label: pJRH-1365 LV EF1a-CD28 IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(116..135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        301..680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        682..880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     835..855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             898..1078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1125..1250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1743..1976
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2161..2205
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2503..2620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2730..3907
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2961..3898
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3855..3875
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4631..5204
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4654..5206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4798..4815)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5025..5044
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5218..5220,5221..5223,5224..5937)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5218..5220,5221..5223,5224..5934)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5263..5284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5524..5543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5871..5892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5952..6540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6005..6025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6423..6434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6612..6845
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6871..6888)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6877..7101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7147..7575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7234..7253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7444..7465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7584..7604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7589..7918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7769..7904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7831..7850
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7966..8013
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8032..8406
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8536..8657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8573..8592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8627..8646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8706..8722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8706..8722)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8719..8741)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8730..8746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8754..8760,8761..8778,8779..8784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8799..8820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8937..8954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9108..9696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9188..9207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9867..10658,10659..10727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10490..10509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10728..10791,1..41))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
       61 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
      121 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      181 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      241 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatatt
      301 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      361 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      601 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      661 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      721 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      781 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      841 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1081 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1141 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1201 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1261 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1321 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1381 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1441 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1501 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1561 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1621 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1681 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1741 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1801 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1861 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1921 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1981 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2041 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2101 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2161 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2221 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2281 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2341 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2401 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2461 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2521 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2581 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2641 cagagatcca gtttggttaa ttaaggtacc atcgatctta agtcgcgact cgagaattcg
     2701 ctagctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     2761 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     2821 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2881 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2941 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     3001 cgggttatgg cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga
     3061 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     3121 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     3181 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     3241 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     3301 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3361 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3421 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3481 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3541 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3601 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3661 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3721 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     3781 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     3841 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     3901 gtcgtgaacc ggttctagag cgctgccacc atgcttcgcc ttcttttggc acttaacctt
     3961 ttccctagta tccaagtaac tgggaataaa atcctggtga aacagtcccc catgctggtc
     4021 gcatacgata acgccgtgaa tctttcatgc aagtatagct ataacctctt cagccgcgaa
     4081 tttcgagcat ctctgcacaa aggtctcgat tccgctgtag aggtctgcgt cgtctacggt
     4141 aactatagtc aacaactcca ggtctatagt aaaaccgggt tcaactgtga tggaaagttg
     4201 ggaaatgaaa gcgttacctt ctacctccaa aacctgtatg taaatcagac ggacatatat
     4261 ttctgcaaaa ttgaagtcat gtatccacca ccttacctgg ataacgagaa gagtaatgga
     4321 accataatac atgtaaaagg caaacacctt tgtccttccc ctctgttccc cggaccctct
     4381 aaacctttct gggttcttgt agtcgttggt ggagtgctcg catgttactc tctgctcgtg
     4441 accgtagctt ttatcatatt ttgggtcagg agcaaacgct cccgactttt gcactccgac
     4501 tatatgaaca tgactccaag acgaccaggg ccaacgcgaa agcactatca accatatgca
     4561 cccccgcgag actttgcagc ttaccgcagt taaacgcgtt aagtcgacaa atgacaagta
     4621 agcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     4681 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     4741 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     4801 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     4861 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4921 acaggtgcct ctgcggccca aagccacgtg tataagatac acctgcaaag gcggcacaac
     4981 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5041 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     5101 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     5161 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg
     5221 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5281 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     5341 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5401 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5461 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5521 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5581 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5641 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5701 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5761 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5821 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5881 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagcgc
     5941 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6001 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6061 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6121 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6181 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6241 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6301 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6361 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6421 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6481 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6541 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6601 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6661 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6721 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6781 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6841 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6901 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6961 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7021 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7081 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7141 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7561 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7621 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7741 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7801 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7861 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7921 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     7981 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     8041 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     8101 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     8161 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     8221 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     8281 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     8341 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     8401 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8461 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8521 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8581 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8641 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     8701 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8761 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8821 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8881 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8941 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9001 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9061 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9541 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9841 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9901 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9961 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10021 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10081 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10141 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10201 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10261 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10321 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10381 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10441 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10501 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10561 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10621 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10681 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10741 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat a
//
