LOCUS       sequence-403604-        8393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli expression of the 53BP1 low-complexity domain (1208-1977) 
            with an N-terminal mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Dumelie JG, Chen Q, Miller D, Attarwala N, Gross SS, Jaffrey SR
  TITLE     Biomolecular condensates create phospholipid-enriched 
            microenvironments.
  JOURNAL   Nat Chem Biol. 2023 Nov 16. doi: 10.1038/s41589-023-01474-4.
  PUBMED    37973889
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Nov 16. doi: 10.1038/s41589-023-01474-4."
COMMENT     Sequence Label: pET28C-mCherry-53BP1LCD
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2371..2388
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2441..2459)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2455..2502
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2539..2994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2626..2645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2836..2857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3086..3901)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3086..3901)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3809..3828
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3893..3912
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4023..4611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4512..4531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4765..4782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4797..4936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4862..4881)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5022..5044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5038..5229)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6038..7120)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7082..7101
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7121..7198)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7404..7423
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7507..7526
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7507..7525
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7526..7550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7565..7587
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7606..7623
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7633..7650
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             7654..7686
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(7693..8393,1..7)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7838..7856)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8106..8125
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtacaagggt gcgcctggtt ctgcaggctc cgcagctggc ggatcgggga tcggtgagaa
       61 accagtcagt gctcctgggg atgatacaga gtcgctccat agccagggag aagaagagtt
      121 tgatatgcct cagcctccac atggccatgt cttacatcgt cacatgagaa caatccggga
      181 agtacgcaca cttgtcactc gtgtcattac agatgtgtat tatgtggatg gaacagaagt
      241 agaaagaaaa gtaactgagg agactgaaga gccaattgta gagtgtcagg agtgtgaaac
      301 tgaagtttcc ccttcacaga ctgggggctc ctcaggtgac ctgggggata tcagctcctt
      361 ctcctccaag gcatccagct tacaccgcac atcaagtggg acaagtctct cagctatgca
      421 cagcagtgga agctcaggga aaggagccgg accactcaga gggaaaacca gcgggacaga
      481 acccgcagat tttgccttac ccagctcccg aggaggccca ggaaaactga gtcctagaaa
      541 aggggtcagt cagacaggga cgccagtgtg tgaggaggat ggtgatgcag gccttggcat
      601 cagacaggga gggaaggctc cagtcacgcc tcgtgggcgt gggcgaaggg gccgcccacc
      661 ttctcggacc actggaacca gagaaacagc tgtgcctggc cccttgggca tagaggacat
      721 ttcacctaac ttgtcaccag atgataaatc cttcagccgt gtcgtgcccc gagtgccaga
      781 ctccaccaga cgaacagatg tgggtgctgg tgctttgcgt cgtagtgact ctccagaaat
      841 tcctttccag gctgctgctg gcccttctga tggcttagat gcctcctctc caggaaatag
      901 ctttgtaggg ctccgtgttg tagccaagtg gtcatccaat ggctactttt actctgggaa
      961 aatcacacga gatgtcggag ctgggaagta taaattgctc tttgatgatg ggtacgaatg
     1021 tgatgtgttg ggcaaagaca ttctgttatg tgaccccatc ccgctggaca ctgaagtgac
     1081 ggccctctcg gaggatgagt atttcagtgc aggagtggtg aaaggacata ggaaggagtc
     1141 tggggaactg tactacagca ttgaaaaaga aggccaaaga aagtggtata agcgaatggc
     1201 tgtcatcctg tccttggagc aaggaaacag actgagagag cagtatgggc ttggccccta
     1261 tgaagcagta acacctctta caaaggcagc agatatcagc ttagacaatt tggtggaagg
     1321 gaagcggaaa cggcgcagta acgtcagctc cccagccacc cctactgcct ccagtagcag
     1381 cagcacaacc cctacccgaa agatcacaga aagtcctcgt gcctccatgg gagttctctc
     1441 aggcaaaaga aaacttatca cttctgaaga ggaacggtcc cctgccaagc gaggtcgcaa
     1501 gtctgccaca gtaaaacctg gtgcagtagg ggcaggagag tttgtgagcc cctgtgagag
     1561 tggagacaac accggtgaac cctctgccct ggaagagcag agagggcctt tgcctctcaa
     1621 caagaccttg tttctgggct acgcatttct ccttaccatg gccacaacca gtgacaagtt
     1681 ggccagccgc tccaaactgc cagatggtcc tacaggaagc agtgaagaag aggaggaatt
     1741 tttggaaatt cctcctttca acaagcagta tacagaatcc cagcttcgag caggagctgg
     1801 ctatatcctt gaagatttca atgaagccca gtgtaacaca gcttaccagt gtcttctaat
     1861 tgcggatcag cattgtcgaa cccggaagta cttcctgtgc cttgccagtg ggattccttg
     1921 tgtgtctcat gtctgggtcc atgatagttg ccatgccaac cagctccaga actaccgtaa
     1981 ttatctgttg ccagctgggt acagccttga ggagcaaaga attctggact ggcaaccccg
     2041 tgaaaatcct ttccagaatc tgaaggtact cttggtatca gaccaacagc agaacttcct
     2101 ggagctctgg tctgagatcc tcatgactgg tggtgcagcc tctgtgaagc agcaccattc
     2161 aagtgcccat aacaaagata ttgctttagg ggtatttgat gtggtggtga cggacccctc
     2221 atgcccagcc tcggtgctga agtgtgctga agcattgcag ctgcctgtgg tgtcacaaga
     2281 gtgggtgatc cagtgcctca ttgttgggga gagaattgga ttcaagcagc atccaaaata
     2341 taaacacgat tatgtttctc actagtcgag caccaccacc accaccactg agatccggct
     2401 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     2461 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     2521 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2581 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2641 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2701 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2761 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2821 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2881 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2941 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3001 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3061 tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca
     3121 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     3181 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     3241 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     3301 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     3361 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     3421 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     3481 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     3541 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     3601 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     3661 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     3721 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     3781 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     3841 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
     3901 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc
     3961 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4021 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4081 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4141 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4201 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4261 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4321 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4381 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4441 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4501 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4561 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4621 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4681 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4741 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4801 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     4861 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     4921 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4981 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5041 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     5101 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     5161 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     5221 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     5281 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     5341 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     5401 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     5461 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     5521 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     5581 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     5641 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc
     5701 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     5761 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     5821 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     5881 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     5941 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     6001 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6061 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6121 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     6181 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     6241 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     6301 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     6361 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     6421 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     6481 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     6541 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     6601 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     6661 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     6721 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     6781 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     6841 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     6901 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     6961 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     7021 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     7081 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     7141 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     7201 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     7261 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     7321 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     7381 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     7441 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
     7501 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     7561 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
     7621 cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatgactgg tggacagcaa
     7681 atgggtcgga tcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     7741 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     7801 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     7861 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     7921 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     7981 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     8041 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     8101 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     8161 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     8221 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     8281 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     8341 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gct
//
