LOCUS       sequence-403662-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Oei V, Chuang LSH, Matsuo J, Srivastava S, Teh M, Ito Y
  TITLE     RUNX3 inactivates oncogenic MYC through disruption of MYC/MAX 
            complex and subsequent recruitment of GSK3beta-FBXW7 cascade.
  JOURNAL   Commun Biol. 2023 Jul 3;6(1):689. doi: 10.1038/s42003-023-05037-0.
  PUBMED    37400551
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-07-3"; volume: "6(1):689. doi"; pages: " 
            10.1038/s42003-023-05037-0"
COMMENT     Sequence Label: pCDNA3-HA-HA-human CMYC (1-277)
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(283..309)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(313..339)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(379..398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(380..398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(443..646)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(472..492)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        647..1026
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1198..1217
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1292..1396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1397..1465,1466..2257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1615..1634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2428..3016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2917..2936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3170..3187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3304..3325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3340..3345,3346..3363,3364..3370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3378..3394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3383..3405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3402..3418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3402..3418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3467..3588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3478..3497)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3532..3551
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3762..4556)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3873..3892)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4483..4502
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4623..4952)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4637..4772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4691..4710)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4937..4957
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4966..5394)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5076..5097)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5288..5307
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5440..5664
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5653..5670
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        5690..5708
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5690..5707
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(5769..6815,1..273))
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKR*ESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
ORIGIN
        1 cccgccaccg ccgtcgttgt ctccccgaag ggagaagggt gtgaccgcaa cgtaggaggg
       61 cgagcagagc ccggagcggc ggctagggga caggggcggg gtgggcagca gctcgaattt
      121 cttccagata tcctcgctgg gcgccggggg ctgcagctcg ctctgctgct gctgctggta
      181 gaagttctcc tcctcgtcgc agtagaaata cggctgcacc gagtcgtagt cgaggtcata
      241 gttcctgttg gtgaagctaa cgttgagggg catggtggat ccggcgtagt cgggcacgtc
      301 gtaagggtac ccggcgtagt cgggcacgtc gtaagggtac atggtgggag atccgagctc
      361 ggtaccaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
      421 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
      481 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      541 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      601 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      661 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
      721 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
      781 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
      841 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
      901 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
      961 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     1021 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     1081 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     1141 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     1201 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     1261 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1621 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3121 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3181 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3241 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3301 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     3361 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3421 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     3481 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3541 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     3601 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     3661 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     3721 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     3781 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3841 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3901 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3961 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     4021 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4081 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4141 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4201 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4261 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4321 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     4381 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     4441 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     4501 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     4561 aacgatcctc atcctgtctc ttgatcagat ccgaaaatgg atatacaagc tcccgggagc
     4621 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     4681 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     4741 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     4801 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     4861 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     4921 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     4981 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     5041 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     5101 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     5161 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     5221 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     5281 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     5341 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     5401 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     5461 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     5521 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     5581 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     5641 atggctggca actagaaggc acagtcgagg ctgatcagcg agctctagca tttaggtgac
     5701 actatagaat agggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg
     5761 cagaattctt acgcacaaga gttccgtagc tgttcaagtt tgtgtttcaa ctgttctcgt
     5821 cgtttccgca acaagtcctc ttcagaaatg agcttttgct cctctgcttg gacggacagg
     5881 atgtatgctg tggctttttt aaggataact accttggggg ccttttcatt gttttccaac
     5941 tccgggatct ggtcacgcag ggcaaaaaag ctccgtttta gctcgttcct cctctggcgc
     6001 tccaagacgt tgtgtgttcg cctcttgaca ttctcctcgg tgtccgagga cctggggctg
     6061 gtgcattttc ggttgttgct gatctgtctc aggactctga cactgtccaa cttgaccctc
     6121 ttggcagcag gatagtcctt ccgagtggag ggaggcgctg cgtagttgtg ctgatgtgtg
     6181 gagacgtggc acctcttgag gaccagtggg ctgtgaggag gtttgctgtg gcctccagca
     6241 gaaggtgatc cagactctca ccttttgcca ggagcctgcc tcttttccac agaaacaaca
     6301 tcgatttctt cctcatcttc ttgttcctcc tcagagtcgc tgctggtggt gggcggtgtc
     6361 tcctcatgga gcaccagggg ctcggggctg ccctgcgggg aggactccgt cgaggagagc
     6421 agagaatccg aggacggaga gaaggcgctg gagtcttgcg aggcgcagga cttgggcgag
     6481 ctgctgtcgt tgagagggta ggggaagacc accgaggggt cgatgcactc tgaggcggcg
     6541 gcgctcagat cctgcaggta caagctggag gtggagcaga cgctgtggcc gcgggcgggg
     6601 ttcgggctgc cgctgtcttt gcgcgcagcc tggtaggagg ccagcttctc tgagacgagc
     6661 ttggcggcgg ccgagaagcc gctccacata cagtcctgga tgatgatgtt tttgatgaag
     6721 gtctcgtcgt ccgggtcgca gatgaaactc tggttcacca tgtctcctcc cagcagctcg
     6781 gtcaccatct ccagctggtc ggccgtggag aagct
//
