LOCUS       sequence-403667-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Oei V, Chuang LSH, Matsuo J, Srivastava S, Teh M, Ito Y
  TITLE     RUNX3 inactivates oncogenic MYC through disruption of MYC/MAX 
            complex and subsequent recruitment of GSK3beta-FBXW7 cascade.
  JOURNAL   Commun Biol. 2023 Jul 3;6(1):689. doi: 10.1038/s42003-023-05037-0.
  PUBMED    37400551
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-07-3"; volume: "6(1):689. doi"; pages: " 
            10.1038/s42003-023-05037-0"
COMMENT     Sequence Label: pCDNA3-HA-HA-human CMYC S62A
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     238..258
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(267..695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(377..398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..608
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    741..965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     954..971
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        991..1009
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     991..1008
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1070..2389)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLAPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             complement(2399..2425)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2429..2455)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2495..2514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2496..2514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2559..2762)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2588..2608)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2763..3142
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3314..3333
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3408..3512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3513..3581,3582..4373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3731..3750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4544..5132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5033..5052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5286..5303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5420..5441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5456..5461,5462..5479,5480..5486)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5494..5510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5499..5521
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5518..5534
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5518..5534
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5583..5704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5594..5613)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5648..5667
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5878..6672)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5989..6008)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6599..6618
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6739..6815,1..253))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6753..6815,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(join(6807..6815,1..11))
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
       61 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
      121 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
      181 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
      241 ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt
      301 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      361 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      421 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
      481 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
      541 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
      601 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
      661 acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct
      721 ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc
      781 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
      841 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
      901 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
      961 cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa tagggccctc
     1021 tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattct tacgcacaag
     1081 agttccgtag ctgttcaagt ttgtgtttca actgttctcg tcgtttccgc aacaagtcct
     1141 cttcagaaat gagcttttgc tcctctgctt ggacggacag gatgtatgct gtggcttttt
     1201 taaggataac taccttgggg gccttttcat tgttttccaa ctccgggatc tggtcacgca
     1261 gggcaaaaaa gctccgtttt agctcgttcc tcctctggcg ctccaagacg ttgtgtgttc
     1321 gcctcttgac attctcctcg gtgtccgagg acctggggct ggtgcatttt cggttgttgc
     1381 tgatctgtct caggactctg acactgtcca acttgaccct cttggcagca ggatagtcct
     1441 tccgagtgga gggaggcgct gcgtagttgt gctgatgtgt ggagacgtgg cacctcttga
     1501 ggaccagtgg gctgtgagga ggtttgctgt ggcctccagc agaaggtgat ccagactctg
     1561 accttttgcc aggagcctgc ctcttttcca cagaaacaac atcgatttct tcctcatctt
     1621 cttgttcctc ctcagagtcg ctgctggtgg tgggcggtgt ctcctcatgg agcaccaggg
     1681 gctcggggct gccctgcggg gaggactccg tcgaggagag cagagaatcc gaggacggag
     1741 agaaggcgct ggagtcttgc gaggcgcagg acttgggcga gctgctgtcg ttgagagggt
     1801 aggggaagac caccgagggg tcgatgcact ctgaggcggc ggcgctcaga tcctgcaggt
     1861 acaagctgga ggtggagcag acgctgtggc cgcgggcggg gttcgggctg ccgctgtctt
     1921 tgcgcgcagc ctggtaggag gccagcttct ctgagacgag cttggcggcg gccgagaagc
     1981 cgctccacat acagtcctgg atgatgatgt ttttgatgaa ggtctcgtcg tccgggtcgc
     2041 agatgaaact ctggttcacc atgtctcctc ccagcagctc ggtcaccatc tccagctggt
     2101 cggccgtgga gaagctcccg ccaccgccgt cgttgtctcc ccgaagggag aagggtgtga
     2161 ccgcaacgta ggagggcgag cagagcccgg agcggcggct aggggccagg ggcggggtgg
     2221 gcagcagctc gaatttcttc cagatatcct cgctgggcgc cgggggctgc agctcgctct
     2281 gctgctgctg ctggtagaag ttctcctcct cgtcgcagta gaaatacggc tgcaccgagt
     2341 cgtagtcgag gtcatagttc ctgttggtga agctaacgtt gaggggcatg gtggatccgg
     2401 cgtagtcggg cacgtcgtaa gggtacccgg cgtagtcggg cacgtcgtaa gggtacatgg
     2461 tgggagatcc gagctcggta ccaagcttgg gtctccctat agtgagtcgt attaatttcg
     2521 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
     2581 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     2641 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     2701 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     2761 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     2821 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     2881 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     2941 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     3001 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     3061 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     3121 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca
     3181 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     3241 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     3301 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
     3361 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3721 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3901 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3961 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4021 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4081 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4141 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4201 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4261 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4321 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4381 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4681 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5221 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5281 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5341 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5401 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5461 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5521 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat
     5581 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5641 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5701 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     5761 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     5821 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     5881 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     5941 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     6001 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     6061 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     6121 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     6181 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     6241 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     6301 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     6361 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     6421 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     6481 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     6541 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     6601 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     6661 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatccga aaatggatat
     6721 acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     6781 ctggaatagc tcagaggcag aggcggcctc ggcct
//
