LOCUS       sequence-403669-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Oei V, Chuang LSH, Matsuo J, Srivastava S, Teh M, Ito Y
  TITLE     RUNX3 inactivates oncogenic MYC through disruption of MYC/MAX 
            complex and subsequent recruitment of GSK3beta-FBXW7 cascade.
  JOURNAL   Commun Biol. 2023 Jul 3;6(1):689. doi: 10.1038/s42003-023-05037-0.
  PUBMED    37400551
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-07-3"; volume: "6(1):689. doi"; pages: " 
            10.1038/s42003-023-05037-0"
COMMENT     Sequence Label: pCDNA3-HA-HA-human CMYC S62D
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..37)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        22..351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      202..337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     264..283
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             418..1212
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(472..491)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1082..1101
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1386..1507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1423..1442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1477..1496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1556..1572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1556..1572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1569..1591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1580..1596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1604..1610,1611..1628,1629..1634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1649..1670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1787..1804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1958..2546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2038..2057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2717..3508,3509..3577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3340..3359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3578..3682)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3757..3776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3948..4327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4328..4531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4482..4502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4576..4595
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4576..4594
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4635..4661
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4665..4691
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4701..6020
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLDPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        complement(6081..6099)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6082..6099)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6119..6136)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6125..6349
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(6395..6815,1..8)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6482..6501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6692..6713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
       61 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
      301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
      361 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
      421 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
      481 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
      541 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
      601 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
      661 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
      721 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
      781 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
      841 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
      901 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
      961 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1021 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1081 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1141 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1201 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     1261 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     1321 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     1381 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1501 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     1561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     3721 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     3781 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     3841 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     3901 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     3961 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4021 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     4081 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     4141 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     4201 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     4261 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     4321 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     4381 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4441 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     4501 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     4561 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg
     4621 gatctcccac catgtaccct tacgacgtgc ccgactacgc cgggtaccct tacgacgtgc
     4681 ccgactacgc cggatccacc atgcccctca acgttagctt caccaacagg aactatgacc
     4741 tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac ttctaccagc
     4801 agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg aagaaattcg
     4861 agctgctgcc caccccgccc ctggacccta gccgccgctc cgggctctgc tcgccctcct
     4921 acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc gggagcttct
     4981 ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg gtgaaccaga
     5041 gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc caggactgta
     5101 tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc tcctaccagg
     5161 ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct
     5221 ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg
     5281 tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg caagactcca
     5341 gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc ccgcagggca
     5401 gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc gactctgagg
     5461 aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg
     5521 gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct cctcacagcc
     5581 cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca gcgcctccct
     5641 ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc agagtcctga
     5701 gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc gaggagaatg
     5761 tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta aaacggagct
     5821 tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag
     5881 ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag caaaagctca
     5941 tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa cttgaacagc
     6001 tacggaactc ttgtgcgtaa gaattctgca gatatccatc acactggcgg ccgctcgagc
     6061 atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     6121 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6181 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6241 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6301 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6361 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6421 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6481 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6541 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6601 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6661 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6721 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6781 tggttaaaaa atgagctgat ttaacaaaaa tttaa
//
