LOCUS       sequence-403683-        9503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of FLAG-S1PR1-GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9503)
  TITLE     Constructs expressing FLAG-S1PR1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-Flag-hS1pr1-GFP-Neo
FEATURES             Location/Qualifiers
     source          1..9503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2300..2323
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3494..3503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3500..3502,3503..3505,3506..4219)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3500..3502,3503..3505,3506..4216)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3545..3566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3806..3825)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4153..4174
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4261..4472
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4485..4753
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4781..5575
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4835..4854)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5445..5464
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5582..6170
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5635..5655)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6053..6064)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6244..6477
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6549..6670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6586..6605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6640..6659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6710..6845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6878..6894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6878..6894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6891..6913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6902..6918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6926..6932,6933..6950,6951..6956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7109..7126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7280..7868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8039..8830,8831..8899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8662..8681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8900..9004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9072..9090
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9128..9150)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9250..9269
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9463..9485
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9477..9494
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9478..9494
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gccaccatgg attacaagga tgacgatgac aaggatatcg gtaccgggcc
     2341 caccagcgtc ccgctggtca aggcccaccg cagctcggtc tctgactacg tcaactatga
     2401 tatcatcgtc cggcattaca actacacggg aaagctgaat atcagcgcgg acaaggagaa
     2461 cagcattaaa ctgacctcgg tggtgttcat tctcatctgc tgctttatca tcctggagaa
     2521 catctttgtc ttgctgacca tttggaaaac caagaaattc caccgaccca tgtactattt
     2581 tattggcaat ctggccctct cagacctgtt ggcaggagta gcctacacag ctaacctgct
     2641 cttgtctggg gccaccacct acaagctcac tcccgcccag tggtttctgc gggaagggag
     2701 tatgtttgtg gccctgtcag cctccgtgtt cagtctcctc gccatcgcca ttgagcgcta
     2761 tatcacaatg ctgaaaatga aactccacaa cgggagcaat aacttccgcc tcttcctgct
     2821 aatcagcgcc tgctgggtca tctccctcat cctgggtggc ctgcctatca tgggctggaa
     2881 ctgcatcagt gcgctgtcca gctgctccac cgtgctgccg ctctaccaca agcactatat
     2941 cctcttctgc accacggtct tcactctgct tctgctctcc atcgtcattc tgtactgcag
     3001 aatctactcc ttggtcagga ctcggagccg ccgcctgacg ttccgcaaga acatttccaa
     3061 ggccagccgc agctctgaga agtcgctggc gctgctcaag accgtaatta tcgtcctgag
     3121 cgtcttcatc gcctgctggg caccgctctt catcctgctc ctgctggatg tgggctgcaa
     3181 ggtgaagacc tgtgacatcc tcttcagagc ggagtacttc ctggtgttag ctgtgctcaa
     3241 ctccggcacc aaccccatca tttacactct gaccaacaag gagatgcgtc gggccttcat
     3301 ccggatcatg tcctgctgca agtgcccgag cggagactct gctggcaaat tcaagcgacc
     3361 catcatcgcc ggcatggaat tcagccgcag caaatcggac aattcctccc acccccagaa
     3421 agacgaaggg gacaacccag agaccattat gtcttctgga aacgtcaact cttcttccgg
     3481 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3541 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3601 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3661 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3721 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3781 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3841 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3901 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3961 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4021 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4081 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4141 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4201 ggacgagctg tacaagtaaa gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt
     4261 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4321 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4381 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     4441 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     4501 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     4561 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     4621 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     4681 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     4741 gccgttacag atccaagctg tgaccggcgc ctacgtcgag atgattgaac aagatggatt
     4801 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4861 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4921 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4981 atcgtggctg gccgcgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5041 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5101 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5161 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5221 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5281 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5341 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5401 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5461 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5521 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgactcga
     5581 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5641 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5701 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5761 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5821 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5881 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5941 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6001 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6061 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6121 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt
     6181 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6241 gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt
     6301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6481 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6541 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6661 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6781 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6841 aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa
     6901 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6961 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7021 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8041 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8101 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8161 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8221 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8281 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8341 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8401 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8461 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8521 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8581 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8641 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8701 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8761 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8821 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8881 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8941 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9001 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9061 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     9121 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     9181 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     9241 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9301 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     9361 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     9421 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     9481 aaacgacggc cagtgccaag ctg
//
