LOCUS       sequence-403707-       13122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant SnRK2 kinase activity FRET sensor in plant expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Zhang L, Takahashi Y, Hsu PK, Kollist H, Merilo E, Krysan PJ, 
            Schroeder JI
  TITLE     FRET kinase sensor development reveals SnRK2/OST1 activation by ABA 
            but not by MeJA and high CO(2) during stomatal closure.
  JOURNAL   eLife. 2020 May 28;9:e56351. doi: 10.7554/eLife.56351.
  PUBMED    32463362
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-05-28"; volume: "9:e56351. doi"; pages: " 
            10.7554/eLife.56351"
COMMENT     Sequence Label: pH7m34GW-35S:SNACS
FEATURES             Location/Qualifiers
     source          1..13122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(821..1000)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1366..1388
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1380..1397
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1381..1397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1405..1425
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1450..1474
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1479..1502)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(join(1701..2213,2214..2216,2217..2219))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             complement(2379..2726)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(2475..2822)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(2571..2918)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(2667..3014)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(2763..3110)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(join(3924..4631,4632..4634,4635..4637))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNKLY"
     CDS             complement(4650..4694)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(4703..4727)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4805..4850)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(4839..4859)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    complement(5562..5582)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5602..5618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5602..5618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5615..5637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     regulatory      5658..5667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5753..5777
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7108..7737
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8166..9239
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9305..9499
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9735..9757)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9843..9983
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9898..9917
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9998..10015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10169..10757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10249..10268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11003..11794)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    12315..12339
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(12432..12684)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(12882..13122,1..785))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 agccttggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
       61 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
      121 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
      181 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
      241 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
      301 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
      361 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
      421 atagcatcca tagcctccgc gaccggctga agaacagcgg gcagttcggt ttcaggcagg
      481 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaac
      541 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
      601 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
      661 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
      721 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
      781 ttcatagggg ggattcgagt tgagagtgaa tatgagactc taattggata ccgaggggaa
      841 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      901 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
      961 agaaacccgc ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1021 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1081 atcgataatt cgagctctcc catatggtcg acctgcaggt cactggattt tggttttagg
     1141 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     1201 atatgctcaa cacatgagcg aaaccctata agaaccctaa ttcccttatc tgggaactac
     1261 tcacacatta ttctggagaa aaatagagag agatagattt gtagagagag actggtgatt
     1321 tttgcggact ctagcatggc cgcggctagt cagttagatc gttttcccag tcacgacgtt
     1381 gtaaaacgac ggccagtgaa ttatcaacta tgtataataa agttgctcga ggtacccggg
     1441 atccaagctt ccactttgta caagaaagct gggtattatt tttcaaattg aggatgagac
     1501 catctagaca gctcgtccat gccgagagtg atcccggcgg cggtcaagaa ctccagcagg
     1561 accatgtgat cgcgcttctc gttggggtct ttgcttaagg cggactgggt gctcaggtag
     1621 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     1681 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
     1741 ttcttctgct tgtcggcggt gatatagacg ttcccgctga tgtagttgta ctccagcttg
     1801 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     1861 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     1921 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     1981 ttcatgtggt cggggtagcg ggcgaagcac tgcacgcccc aggacagggt ggtcacgagg
     2041 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     2101 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     2161 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     2221 cggccgcgat ctaaaaactc ttcgattgat tcaccgaaac tcgaaaagta cgaacttggt
     2281 gctgattggt aacgaatcag tcctgagttt cttggtctct ggtgttgatg atcgtggaga
     2341 aggaagtgat gttgctggaa ttctgattcc attccggaac caccagcact accaccagca
     2401 ctaccaccag cactaccacc agcactacca ccagcactgg tactaccacc agcactacca
     2461 ccagcactgc cggaaccacc agcactacca ccagcactac caccagcact accaccagca
     2521 ctaccaccag cactggtact accaccagca ctaccaccag cactgccgga accaccagca
     2581 ctaccaccag cactaccacc agcactacca ccagcactac caccagcact ggtactacca
     2641 ccagcactac caccagcact gccggaacca ccagcactac caccagcact accaccagca
     2701 ctaccaccag cactaccacc agcactggta ctaccaccag cactaccacc agcactgccg
     2761 gaaccaccag cactaccacc agcactacca ccagcactac caccagcact accaccagca
     2821 ctggtactac caccagcact accaccagca ctgccggaac caccagcact accaccagca
     2881 ctaccaccag cactaccacc agcactacca ccagcactgg tactaccacc agcactacca
     2941 ccagcactgc cggaaccacc agcactacca ccagcactac caccagcact accaccagca
     3001 ctaccaccag cactggtact accaccagca ctaccaccag cactgccgga accaccagca
     3061 ctaccaccag cactaccacc agcactacca ccagcactac caccagcact ggtaccgatc
     3121 tccttctgtt cttcagcagg ctttggtgct gctgcttctt taatctcctc cggactttcg
     3181 tcctgcatgt cagaagtcca gagagtaaga ttgtcacgaa gaagctgcat gatcaaggta
     3241 ctatccttgt atgactcctc tccaagagta tctaactcag caatggcttc atcaaacgcc
     3301 tgcttagcga gattacaagc acgatctgga gaattaagaa tctcatagta aaacacagag
     3361 aaattcaaag ccaaaccaag acgaatcgga tgcgttggag ccaattcagc attagcaata
     3421 tcctgagcag ctttgtaagc ggtgagagta tgttcagcag catctttcct ctcttgacca
     3481 gtcttaaact cagccaaata cctatgataa tctcccttca tcttaaggta aaaaacctta
     3541 gaatctccat tagcagaagc aggaacaagt ctagtatcaa gaagcttaag aataccgtca
     3601 cagattttcg ataactcaga ttcgatcttg cttctgtaat cacggatcgt ggtcacatgg
     3661 tcatcgttac cacgactctc ttctttttgt tcaatggaag agatgattct ccaggaagca
     3721 cgacgagcgc cgattacgtt tttgtaagcg acggagagga gattacgttc ttcgacggtg
     3781 agttcgtctt tgtcaacggc ttcagcgact ttttccatta attcaaccat ttcttcgtaa
     3841 cgttccgctt gctctgcgag ctttgcgagg tacacgaact cttccctcgc cgaggatgat
     3901 gctggtggtg ccgccatctc gaggtacagc ttgttcatgc cctcagtgat cccggcggcg
     3961 gtcaggaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gaacagggcg
     4021 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     4081 ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg
     4141 aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg
     4201 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     4261 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     4321 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     4381 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag
     4441 cccagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcatagaagc
     4501 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     4561 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     4621 tcgcccttgc tcaccatgcc gaattctggg ctgtccatgt gctggcgttc gaatttagca
     4681 gcagcggttt ctttcatggt gaagcctgct tttttgtaca aacttgccaa attgtaaata
     4741 gtaattgtaa tgttgtttgt tgtttgttgt tgttggtaat tgttgtaaaa atactctaga
     4801 ccggcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     4861 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     4921 acgtggttgg aacgtcttct ttttccacga tgttcctcgt gggtgggggt ccatctttgg
     4981 gaccactgtc ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     5041 tgtagaagcc atcttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat
     5101 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctctggt
     5161 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     5221 tgaccatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
     5281 atgttcctcg tgggtggggg tccatctttg ggaccactgt cggtagaggc atcttgaacg
     5341 atagcctttc ctttatcgca atgatggcat ttgtagaagc catcttcctt ttctactgtc
     5401 ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
     5461 ctttgttgaa aagtctcaat agccctctgg tcttctgaga ctgtatcttt gatattcttg
     5521 gagtagacga gagtgtcgtg ctccaccatg ttggccgcga gcaacttttc tatacaaagt
     5581 tgatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcgg
     5641 gcccgcggat cccgcggcca tggcggccgg gagcatgcga cgtcgggccc aagcttagct
     5701 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     5761 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     5821 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     5881 ctcgggatca aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc
     5941 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     6001 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     6061 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     6121 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     6181 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     6241 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     6301 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     6361 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     6421 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     6481 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     6541 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     6601 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     6661 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     6721 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     6781 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     6841 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     6901 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     6961 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     7021 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     7081 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     7141 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     7201 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     7261 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     7321 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     7381 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     7441 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     7501 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     7561 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     7621 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     7681 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     7741 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     7801 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     7861 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     7921 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     7981 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     8041 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     8101 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     8161 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     8221 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     8281 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     8341 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     8401 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     8461 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     8521 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     8581 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     8641 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     8701 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     8761 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     8821 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     8881 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     8941 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     9001 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     9061 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     9121 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     9181 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     9241 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     9301 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     9361 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     9421 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     9481 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     9541 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     9601 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     9661 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     9721 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9781 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9841 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     9901 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9961 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    10621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10801 acgaaaactc acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg
    10861 cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc
    10921 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
    10981 tgaacgaatt tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
    11041 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
    11101 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
    11161 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
    11221 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
    11281 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
    11341 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
    11401 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
    11461 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
    11521 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
    11581 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
    11641 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
    11701 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
    11761 gagtcgatac ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac
    11821 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
    11881 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag
    11941 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
    12001 tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact
    12061 gggttcgtgc ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    12121 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    12181 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    12241 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    12301 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    12361 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt
    12421 gcatgccggt cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    12481 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    12541 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    12601 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    12661 ttattgccaa atgtttgaac gatctgcttg actctaggga attaattcct gaatcactgc
    12721 gaccggccct cccgcgaccc agccgagcga gcttagcgaa ctgtggacga gaactgtgcc
    12781 accaagcgta aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg
    12841 aggcgttctc gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga
    12901 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    12961 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    13021 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    13081 tgatagagtt ggtcaagacc aatgcggagc atatacgccc gg
//
