LOCUS       sequence-403708-       12450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant SnRK2S30A kinase activity FRET sensor in plant expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Zhang L, Takahashi Y, Hsu PK, Kollist H, Merilo E, Krysan PJ, 
            Schroeder JI
  TITLE     FRET kinase sensor development reveals SnRK2/OST1 activation by ABA 
            but not by MeJA and high CO(2) during stomatal closure.
  JOURNAL   eLife. 2020 May 28;9:e56351. doi: 10.7554/eLife.56351.
  PUBMED    32463362
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-05-28"; volume: "9:e56351. doi"; pages: " 
            10.7554/eLife.56351"
COMMENT     Sequence Label: pH7m34GW-35S:SNACS S785A
FEATURES             Location/Qualifiers
     source          1..12450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1114..1366
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1459..1483
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2004..2795
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3041..3629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3530..3549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3783..3800
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3815..3955
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3881..3900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4041..4063
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4299..4493
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4559..5632)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6061..6690)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8021..8045
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     regulatory      8131..8140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     8161..8183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8180..8196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8180..8196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    8216..8236
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     8939..8959
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        8948..8993
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    9071..9095
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9104..9148
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             join(9161..9163,9164..9166,9167..9874)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNKLY"
     CDS             join(10907..10909,10910..10912,10913..11425)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             11624..11647
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(11652..11676)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11701..11721)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11729..11746)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11729..11745)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11738..11760)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        12126..12305
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(12341..12450,1..916)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
       61 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      121 gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
      181 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttcttcagc cggtcgcgga
      241 ggctatggat gctatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
      301 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
      361 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
      421 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
      481 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
      541 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
      601 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
      661 aggatcgcca aggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
      721 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
      781 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
      841 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
      901 gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag aacgcctcag
      961 ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac gcttggtggc
     1021 acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg gccggtcgca
     1081 gtgattcagg aattaattcc ctagagtcaa gcagatcgtt caaacatttg gcaataaagt
     1141 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     1201 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     1261 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     1321 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc ggcatgcaag
     1381 ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     1441 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     1501 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     1561 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     1621 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     1681 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     1741 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1801 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1861 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1921 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1981 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     2041 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2101 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2161 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2221 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2281 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2341 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2401 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2461 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2521 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2581 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2641 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2701 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2761 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     2821 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     2881 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     2941 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3181 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3781 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3841 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3901 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     3961 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4021 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4081 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     4141 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     4201 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     4261 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     4321 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     4381 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     4441 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     4501 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     4561 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     4621 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     4681 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     4741 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     4801 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     4861 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     4921 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
     4981 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
     5041 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
     5101 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
     5161 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
     5221 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
     5281 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
     5341 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
     5401 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
     5461 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
     5521 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
     5581 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
     5641 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
     5701 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
     5761 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
     5821 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
     5881 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
     5941 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
     6001 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
     6061 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
     6121 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
     6181 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
     6241 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
     6301 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
     6361 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
     6421 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
     6481 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
     6541 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
     6601 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
     6661 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
     6721 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
     6781 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
     6841 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
     6901 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
     6961 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
     7021 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
     7081 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
     7141 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
     7201 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
     7261 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
     7321 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
     7381 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
     7441 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
     7501 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
     7561 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
     7621 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
     7681 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
     7741 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
     7801 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
     7861 gttggatcaa agtactttaa agtactttaa agtactttaa agtactttga tcccgagggg
     7921 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
     7981 taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
     8041 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
     8101 taagcttggg cccgacgtcg catgctcccg gccgccatgg ccgcgggatc cgcgggcccg
     8161 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctatcaact
     8221 ttgtatagaa aagttgctcg cggccaacat ggtggagcac gacactctcg tctactccaa
     8281 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt
     8341 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     8401 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
     8461 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt
     8521 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atggtcaaca
     8581 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     8641 agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     8701 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat
     8761 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca
     8821 aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt
     8881 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     8941 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggccggtct
     9001 agagtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattactat
     9061 ttacaatttg gcaagtttgt acaaaaaagc aggcttcacc atgaaagaaa ccgctgctgc
     9121 taaattcgaa cgccagcaca tggacagccc agaattcggc atggtgagca agggcgagga
     9181 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     9241 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct
     9301 tctatgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta
     9361 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     9421 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     9481 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     9541 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     9601 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     9661 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     9721 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     9781 cctgttcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcctgaccgc
     9841 cgccgggatc actgagggca tgaacaagct gtacctcgag atggcggcac caccagcatc
     9901 atcctcggcg agggaagagt tcgtgtacct cgcaaagctc gcagagcaag cggaacgtta
     9961 cgaagaaatg gttgaattca tggaaaaagt cgctgaagcc gttgacaaag acgaactcac
    10021 cgtcgaagaa cgtaatctcc tctccgtcgc ttacaaaaac gtaatcggcg ctcgtcgtgc
    10081 ttcctggaga atcatctctt ccattgaaca aaaagaagag agtcgtggta acgatgacca
    10141 tgtgaccacg atccgtgatt acagaagcaa gatcgaatct gagttatcga aaatctgtga
    10201 cggtattctt aagcttcttg atactagact tgttcctgct tctgctaatg gagattctaa
    10261 ggttttttac cttaagatga agggagatta tcataggtat ttggctgagt ttaagactgg
    10321 tcaagagagg aaagatgctg ctgaacatac tctcaccgct tacaaagctg ctcaggatat
    10381 tgctaatgct gaattggctc caacgcatcc gattcgtctt ggtttggctt tgaatttctc
    10441 tgtgttttac tatgagattc ttaattctcc agatcgtgct tgtaatctcg ctaagcaggc
    10501 gtttgatgaa gccattgctg agttagatac tcttggagag gagtcataca aggatagtac
    10561 cttgatcatg cagcttcttc gtgacaatct tactctctgg acttctgaca tgcaggacga
    10621 aagtccggag gagattaaag aagcagcagc accaaagcct gctgaagaac agaaggagat
    10681 cggtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
    10741 tggtggttcc ggaatggaat cagaattcca gcaacatcac ttccttctcc acgatcatca
    10801 acaccagaga ccaagaaact caggattgat tcgttaccaa gcagcaccaa gttcgtactt
    10861 ttcgagtttc ggtgaatcaa tcgaagagtt tttagatcgc ggccgcatgg tgagcaaggg
    10921 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
    10981 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
    11041 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
    11101 gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
    11161 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
    11221 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
    11281 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
    11341 ctacatcagc gggaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
    11401 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
    11461 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
    11521 gtccgcctta agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttctt
    11581 gaccgccgcc gggatcactc tcggcatgga cgagctgtct agatggtctc atcctcaatt
    11641 tgaaaaataa tacccagctt tcttgtacaa agtggaagct tggatcccgg gtacctcgag
    11701 caactttatt atacatagtt gataattcac tggccgtcgt tttacaacgt cgtgactggg
    11761 aaaacgatct aactgactag ccgcggccat gctagagtcc gcaaaaatca ccagtctctc
    11821 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
    11881 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
    11941 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
    12001 cagtgacctg caggtcgacc atatgggaga gctcgaatta tcgatcatga gcggagaatt
    12061 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac
    12121 tgacagaacc gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct
    12181 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
    12241 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
    12301 aattagagtc tcatattcac tctcaactcg aatcccccct atgaaaaagc ctgaactcac
    12361 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
    12421 gctctcggag ggcgaagaat ctcgtgcttt
//
