LOCUS       sequence-403731-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p62 K7A/D69A in a doxycycline-dependent manner in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, 
            Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H,
            Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M
  TITLE     Integrated proteomics identifies p62-dependent selective autophagy 
            of the supramolecular vault complex.
  JOURNAL   Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015.
  PUBMED    37192622
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015."
COMMENT     Sequence Label: pRetroX-TetOne-Puro-p62 K7A/D69A
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    949..1306
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1717..1739
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1755..1771
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2109..2125
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     polyA_signal    2273..2407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3941..4308)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4066..4084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4102..4120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4138..4156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4174..4192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4210..4228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4246..4264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4282..4300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4325..4835
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4754..4772
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4854..5600
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        5618..5947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5798..5933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5956..6555
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5956..5975)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6572..6975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    7995..8116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8139..8156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8139..8155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8148..8170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8364..8383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8483..8505
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8543..8561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8629..8733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8734..8802,8803..9594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8952..8971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9765..10353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10254..10273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10507..10524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10641..10662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10677..10682,10683..10700,10701..10707)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10715..10731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10720..10742
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10739..10755
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10739..10755
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcctaataca tcccaaactc aaatatataa agcatttgac ttgttctatg ccctaggagc
       61 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
      121 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 ccgccccatt gacgcaaatg ggcatctaga gcgcttctgc tccccgagct ctataaaaga
      721 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
      781 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
      841 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
      901 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc
      961 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
     1021 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
     1081 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
     1141 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
     1201 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     1261 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
     1321 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tgagggcccg ggctagcctg
     1381 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
     1441 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
     1501 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
     1561 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
     1621 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
     1681 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
     1741 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
     1801 atgagatcct atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
     1861 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
     1921 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
     1981 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
     2041 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
     2101 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
     2161 cctctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2221 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2281 tattgcagct tataatggtt acaaataagg caatagcatc acaaatttca caaataaggc
     2341 atttttttca ctgcattcta gttttggttt gtccaaactc atcaatgtat cttatcatgt
     2401 ctggatctca aatccctcgg aagctgcgcc tgtcttaggt tggagtgata catttttatc
     2461 acttttaccc gtctttggat taggcagtag ctctgacggc cctcctgtct taggttagtg
     2521 aaaaatgtca ctctcttacc cgtcattggc tgtccagctt agctcgcagg ggaggtggtc
     2581 tggatccgcc ggcaccggtg tatacgggaa tttcacaacg gcgggggatg ctttgaatac
     2641 tggatggtgt ccagagccgc tccgatgtca tagttcttgg tctgcaggag cctggtgagc
     2701 cagccgcctt catcagagaa gcccatggac agcatctggg agagggactc aatcagccgc
     2761 gggtcagcct ctggcgggag atgtgggtac aaggcagctt ccttcagccc tgtgggtccc
     2821 tcctgggagg ggtccagaga gcttggccct tcggattctg gcatctgtag ggactggagt
     2881 tcacctgtag acgggtccac ttcttttgaa gacagatggg tccagtcatc atctcctcct
     2941 gagcaattgt ctgattccat ttgttcctca gggcgcccct cggactccaa ggcgatcttt
     3001 ctcatctgct ccgccagaga ctgcgtggcg ccctcaacat tcccacccgg cttgctggga
     3061 tcagagcagc agctgcttgg ctgtgagctg ctcttctcct ctgtgctgga actctctgga
     3121 gagacggggg tcaggcggct tcttttccct ccgtgctcca catcgatatc aacttcaatg
     3181 cccagagggc taagggcagc tgccacactc tccccaacgt tcttcaggaa attcacactc
     3241 ggatcctccg atggaccaga agctgattct gccgtggggc cagggcgggc ctcccctgca
     3301 cgaggaggac gtgggctcca gtttcctggt ggacccattt cccatcctgg ccacccgaag
     3361 tgtccgtgtt tcaccttccg gagccagcgg ctgtgcgaga agccctcaga caggtgcccg
     3421 aaggggctgg ggaatgcgag cttggtgtgc ccccggtgca agccctttcc ctcgcagacg
     3481 ctacacaagt cgtagtctgg gcagacgctg cacttgtagc gggttcctac cacaggccca
     3541 ttgcagccat cgcagatcac attggggtgc accatgttgc ggggcgcctc ctgagcacac
     3601 ggtgggcggt ggtcccgccg gcactctttt ttctctttaa tgtagattcg gaagatgtca
     3661 tccttcacgt aggacatggc cattgtcaat tcctcgtcac tggaaaaggc aaccaagtcc
     3721 ccgtcctcag cgcggtagtg cgcctggaag ccgccaggcc gcagcgcggg gaacagggcg
     3781 gccacccggc tcagcagccg ctcgcagggt cccggacccg ccgcagcctc ggcttccgcc
     3841 tcaggctcgg ggctgcagca gaagctgaag cggcgaatct cgcgcgccgc gtcctccttg
     3901 cccagaaggt aggccgccac ggtgagcgac gccataattc tttacgaggg taggaagtgg
     3961 tacggaaagt tggtataaga caaaagtgtt gtggaattga agtttactca aaaaatcagc
     4021 actcttttat aggcgccctg gtttacataa gcaaagctta tacgttctct atcactgata
     4081 gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct
     4141 ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc
     4201 cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata
     4261 gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga ggtgataatt
     4321 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     4381 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     4441 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     4501 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     4561 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     4621 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     4681 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     4741 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     4801 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta
     4861 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
     4921 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     4981 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     5041 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     5101 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     5161 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     5221 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     5281 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     5341 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
     5401 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     5461 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     5521 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     5581 ttgacatgct ccccgggtaa ctaagtaaga attgaatgtg tgtcagttag ggtgtggaaa
     5641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5701 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5761 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5821 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5881 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5941 ttgcaaaacg cgaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6001 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6061 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6121 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6181 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6241 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6301 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6361 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6421 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6481 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6541 aagcccggtg cctgaacgcg taattcctgt taatcaacct ctggattaca aaatttgtga
     6601 aagattgact gatattctta actatgttgc tccttttacg ctgtgtggat atgctgcttt
     6661 aatgcctctg tatcatgcta ttgcttcccg tacggctttc gttttctcct ccttgtataa
     6721 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtccgtcaac gtggcgtggt
     6781 gtgctctgtg tttgctgacg caacccccac tggctggggc attgccacca cctgtcaact
     6841 cctttctggg actttcgctt tccccctccc tatcgccacg gcagaactca tcgccgcctg
     6901 ccttgcccgc tgctggacag gggctaggtt gctgggcact gataattccg tggtgttgtc
     6961 ggggaagctg acgtcgagat cttcctgcgg agatatcagt ggtccaggct ctagttttga
     7021 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     7081 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7141 cttctcgctt ctgttcgcgc gcttctgctc ccagacatca acaaaagagc ccacaacccc
     7201 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     7261 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     7321 gtgattgact acccgtcagc gggggtcttt cacgcgtatg ccgcatagtt aagccaccgc
     7381 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     7441 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     7501 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     7561 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     7621 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     7681 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     7741 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     7801 gagcgcacga ccccatggct ccgaccgaag ccacccgggg cggccccgcc gaccccgcac
     7861 ccgcccccga ggcccaccga ctctagagga tcataatcag ccataccaca tttgtagagg
     7921 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7981 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8041 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8101 tcatcaatgt atcttatcat ggcgcgtcga attaattcac tggccgtcgt tttacaacgt
     8161 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8221 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8281 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8341 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8401 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8461 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8521 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8581 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8641 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8701 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8761 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8821 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8881 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8941 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9001 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9061 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9121 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9181 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9241 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9301 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9361 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9421 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9481 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9541 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9601 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9661 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9781 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9841 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9901 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9961 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10021 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10081 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10141 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10201 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10261 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10321 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10381 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10441 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10501 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10561 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10621 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10681 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10741 ggaaacagct atgaccatga ttacgccaag ctagctttgc tcttaggagt t
//
