LOCUS       sequence-403741-       10416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST TNRC6A
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        480..683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     634..654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     728..747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        728..746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             819..842
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             843..869
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    885..909
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5138..5149
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(6077..6101)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6154..6195
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6233..6250)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6239..6463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6596..6615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6747..6794
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6802..7824
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6802..7824
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7954..8075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7991..8010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8045..8064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8124..8140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8124..8140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8137..8159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8148..8164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8172..8178,8179..8196,8197..8202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8217..8238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8355..8372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8526..9114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8606..8625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9285..10076,10077..10145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9908..9927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10146..10250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10325..10344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      721 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgagct cggtcgatcc
      781 atcgatagtg tggtggaatt ctgatcgccg ccgccatgga ctacaaggac gacgatgaca
      841 agtaccctta tgacgtgccc gattacgctt tgaaccagat atcaacaagt ttgtacaaaa
      901 aagcaggctc caccatggga accatggatg ctgattctgc ctccagttct gaatcagaga
      961 gaaacatcac tatcatggct tcagggaaca caggtggtga aaaagatggc cttcggaata
     1021 gcactggact tggttcccaa aacaagtttg tagttggtag cagcagcaat aatgtgggcc
     1081 atggaagtag tactgggcca tggggttttt cccatggagc cataataagc acatgtcagg
     1141 tctctgtgga tgctcctgaa agcaaatctg aaagtagcaa caatagaatg aatgcttggg
     1201 gcactgtaag ttcttcatca aatggagggt taaatccaag cactttgaat tcagctagca
     1261 accatggtgc ctggccagta ttagagaaca atggacttgc cctaaaaggg cctgtaggga
     1321 gtggtagttc tggcattaat attcagtgca gtactatagg ccagatgcct aacaatcaga
     1381 gtattaactc taaagtgagt ggtggttcta cccatggtac ctggggaagc cttcaggaaa
     1441 cttgtgaatc tgaagtaagt ggtacacaga aggtttcatt cagtggtcaa cctcaaaata
     1501 ttaccactga aatgactgga ccaaataaca ctactaactt tatgacctct agtttaccaa
     1561 actccggttc agtgcagaat aatgagctgc ctagtagtaa cacaggggcc tggcgtgtga
     1621 gcacaatgaa tcatcctcag atgcaggctc catcaggtat gaatggcact tccctttctc
     1681 accttagcaa tggagagtca aaaagtggag gctcttatgg tactacatgg ggtgcctatg
     1741 gttctaatta ctctggagac aaatgttcag gccctaatgg ccaagctaat ggtgacactg
     1801 tgaatgcaac tctaatgcag cctggcgtaa atggtcctat gggcactaac tttcaagtta
     1861 acacaaacaa aggaggtggt gtgtgggaat ctggtgcagc aaactcccag agtacatcat
     1921 ggggaagtgg aaatggcaca aattctggag gaagtcgaag aggatgggga acccctgcac
     1981 aaaacactgg cactaattta cccagcgttg agtggaacaa actgcctagc aatcagcatt
     2041 ccaatgatag tgcaaatggc aatggtaaga cgtttacaaa tggatggaaa tctactgagg
     2101 aagaggatca gggttctgcc acatctcaga caaatgagca aagcagtgtg tgggccaaaa
     2161 caggaggtac agtggagagc gatggtagta cagaaagcac tggacgcctt gaggaaaaag
     2221 gaactgggga aagtcagagt agagacagaa gaaaaattga tcagcacaca ttactccaaa
     2281 gcattgtaaa cagaactgac ttagatccac gtgtcctgtc caactctggt tggggacaga
     2341 ctcctattaa gcagaatact gcctgggata cagaaacatc acctagaggg gaacgaaaga
     2401 ctgacaatgg gacagaggcc tggggaagct ctgcaacaca gacttttaac tcaggggcat
     2461 gtatagataa gactagccct aatggtaatg atacctcatc tgtatcaggg tggggcgatc
     2521 ccaaacctgc tctgaggtgg ggagattcca aaggctcaaa ctgccagggg gggtgggaag
     2581 atgattctgc tgctacagga atggtcaaga gcaatcagtg ggggaattgc aaagaggaga
     2641 aggctgcatg gaatgactcg caaaagaata aacagggatg gggtgatgga caaaaatcaa
     2701 gccaagggtg gtctgtttct gccagtgata actggggaga aacttcaagg aataaccatt
     2761 ggggtgaggc caataagaaa tccagctcag gaggtagtga cagtgacagg tccgtttccg
     2821 gttggaacga acttggtaaa actagttctt tcacttgggg aaacaacata aatccaaata
     2881 attcatcagg atgggatgaa tcttctaaac ctactccttc ccagggatgg ggagaccctc
     2941 caaagtctaa tcagtctcta ggttggggag attcgtcaaa gccagtcagc tctccagact
     3001 ggaacaagca acaagacatt gttggatctt ggggaatccc accagctaca ggcaaacctc
     3061 ctggtacagg ctggctgggg ggacctatac cagccccagc aaaagaagaa gaacccacag
     3121 gctgggagga accatcccca gaatctatac gtcgcaaaat ggagattgat gatggaactt
     3181 cagcttgggg agatccaagc aaatacaact acaaaaatgt gaacatgtgg aacaaaaacg
     3241 tcccaaatgg caacagccgt tcagaccagc aagcacaggt acatcagctg ctaacgcctg
     3301 caagtgccat ctcaaacaaa gaggcaagca gtggctctgg ctggggtgag ccctgggggg
     3361 agccttctac tccagccaca actgtggata atggtacttc agcatggggt aagcccatag
     3421 acagtggtcc cagctggggg gaacccattg ctgcggcatc cagcacatcc acgtggggct
     3481 ccagctctgt tggtccacaa gcattaagca aatctgggcc aaaatctatg caagatggct
     3541 ggtgtggtga tgatatgcca ttgcctggaa atcgccccac tggctgggaa gaggaagagg
     3601 atgtggagat tggaatgtgg aatagtaatt catctcaaga gcttaactca tctttaaatt
     3661 ggccaccata tacaaagaaa atgtcatcga agggtctgag tggcaaaaaa aggagaaggg
     3721 aaaggggaat gatgaaaggt ggaaacaaac aagaagaagc gtggataaat ccatttgtta
     3781 aacagttttc aaacatcagt ttttcgagag actcaccaga ggaaaatgta caaagcaata
     3841 agatggacct ttctggagga atgttacaag acaaacgaat ggagatagat aaacatagcc
     3901 taaatattgg tgattacaat cgaacggtcg ggaaaggccc tggttctcgg cctcagattt
     3961 ccaaagagtc ttccatggag cgcaatcctt attttgataa ggatggcatt gtagcagatg
     4021 aatcccaaaa catgcagttt atgtccagtc aaagcatgaa gcttccccct tcaaatagtg
     4081 cactacctaa ccaggccctt ggctccatag cagggctggg tatgcaaaac ttgaattctg
     4141 ttagacagaa tggcaatccc agtatgtttg gtgttggaaa cacagcagca caaccccggg
     4201 gcatgcagca gcctccagca caacctctta gttcatctca gcctaatctc cgtgctcaag
     4261 tgcctcctcc attactctcc cctcaggttc cagtttcatt gctgaagtat gcaccaaaca
     4321 acggtggcct gaatccactc tttggccctc aacaggtagc catgctgaac cagctatccc
     4381 agctaaacca gctttctcag atctcccagt tacagcgatt gttagcgcag cagcaaaggg
     4441 cgcagagtca gagaagcgtg ccttctggga accggccgca gcaagaccag cagggtcgac
     4501 ctcttagtgt gcagcagcaa atgatgcaac aatctcgtca acttgatcca aacctgttgg
     4561 tgaagcagca gactccacca tctcagcagc agccactcca tcagccagcc atgaagtctt
     4621 tccttgacaa tgtcatgccc cacactacac ctgagctgca aaaagggcca tcaccaataa
     4681 atgctttcag caacttccct ataggcttga actcaaactt gaatgtaaat atggatatga
     4741 acagtattaa agagccacag tcaagactaa ggaagtggac gacagtggac agcatttctg
     4801 tgaacacatc tttggatcaa aactccagca aacatggtgc tatttcaagt ggtttcaggc
     4861 tggaagagtc tccatttgtt ccctatgact ttatgaacag cagtacttca ccagccagtc
     4921 ctccaggttc aataggagat ggctggccac gtgccaaatc gcctaacggc tctagcagtg
     4981 ttaattggcc accagaattt cgtcctggtg agccatggaa aggttatcca aacattgacc
     5041 ctgaaactga cccttacgtc actcctggca gtgtcataaa caatctttca attaatactg
     5101 tgcgggaagt tgaccacctc agggacagga acagtgggtc atcctcatcc ttgaacacca
     5161 cgctgccttc aactagtgcc tggtcatcca ttcgtgcctc caactacaac gttcccctca
     5221 gcagtacagc acaaagcact tcagccagaa atagtgattc caaattgaca tggtctcctg
     5281 gttcagttac aaacacctct ctggctcatg agctgtggaa ggtccctttg ccacctaaaa
     5341 acatcactgc tccgtcccgc ccacctccgg gactgactgg tcagaagcca cccttgtcta
     5401 cgtgggataa ttctcccctt cgtataggtg gaggatgggg aaattctgac gccagatata
     5461 ccccaggttc cagctggggt gagagcagct cagggagaat aacaaattgg cttgttctaa
     5521 aaaaccttac acctcagatc gatggctcaa ctctgcgcac tctgtgcatg cagcacggcc
     5581 cgctgatcac attccacctg aacctccctc acggaaatgc tctggtccgc tacagttcaa
     5641 aagaagaggt agtgaaggca caaaagtctc tgcacatgtg tgtactgggg aacactacta
     5701 ttcttgctga gtttgccagt gaagaggaga tcagtcgttt ctttgcacaa agccagtctc
     5761 tgaccccttc tcccggctgg cagtctctcg ggtccagcca gagccggctg ggctccctcg
     5821 actgttccca ctcattctcc agccggaccg atctcaatca ctggaatggt gctgggctgt
     5881 cgggaactaa ctgtggagac cttcacggca cttcactctg ggggaccccg cattattcca
     5941 caagcctgtg gggtccccca agcagcagcg acccccgagg aattagcagc ccatctccca
     6001 ttaacgcttt tctttctgtt gaccacctgg gtgggggtgg agagtccatg taagcggccg
     6061 cactcgagat atctagaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
     6121 ggccgctcaa gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     6181 ggtctcgatt ctacgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     6241 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6301 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6361 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6421 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6481 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6601 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6661 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6721 gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa
     6781 agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     6841 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     6901 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     6961 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     7021 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     7081 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     7141 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     7201 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     7261 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     7321 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     7381 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     7441 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     7501 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     7561 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     7621 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     7681 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     7741 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     7801 actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc
     7861 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7921 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8041 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8101 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10261 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    10321 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    10381 tggaggtcgc tgagtagtgc gcgagcaaaa tttaag
//
