LOCUS       sequence-403745-       12436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC 
            cassette-35S-bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG159
FEATURES             Location/Qualifiers
     source          1..12436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2597..3148
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3150..3402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3723..3769
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(5637..5654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5637..5653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5646..5668)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5875..5899
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6420..7211
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7457..8045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7946..7965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8199..8216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8231..8371
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8297..8316)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8457..8479
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8715..8909
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8975..10048)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10477..11106)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctatga gcccagaacg acgcccggcc gacatccgcc gtgccaccga
     2641 ggcggacatg ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa
     2701 cttccgtacc gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga
     2761 gcgctatccc tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg
     2821 accctggaag gcacgcaacg cctacgactg gacggccgag tcgaccgtgt acgtctcccc
     2881 ccgccaccag cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga
     2941 ggcacagggc ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg
     3001 catgcacgag gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca
     3061 cgggaactgg catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg
     3121 tccggtcctg cccgtcaccg agatctaacg atcgttcaaa catttggcaa taaagtttct
     3181 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3421 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3481 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     3541 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     3601 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     3661 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     3721 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     3781 tagtcgactc tagcctcgag gagctcacca tgtactctcc tatggtcgta ccgcaaaaaa
     3841 atgtgcctct ccctatagac gataaatcta gctccatgga gcttccgcag atccacgcag
     3901 acactatcgg tcgattggtg ctggaaaagt tcatggaggt tattaaaggt caggaggctt
     3961 atagccgtcg aaaagttttg gctggaatcg taatgaccga aaacatgaac ttctgtgagg
     4021 caaaagtcat ttcagtgtcc acgggcacca agtgtgtgtc cggcgagcat atgtccgtaa
     4081 acggtgcggt actcaatgac agccacgccg agatagtgtc acgtcgttgt ctcctcaagt
     4141 atctctatgc ccaactggat cttcaatgca accaggcgac cgcttatcag agcatatttg
     4201 tgcgtaatac tgacggtcag tatccctata aactgaagtc tggggtccat tttcatctct
     4261 atataaacac ggcgccctgc ggcgatgccc gtattttttc acctcacgag aatgacacgg
     4321 gagtagataa acatcccaac cgaaaagcaa gagggcagct ccgaaccaaa atcgaatccg
     4381 gccagggcac aatcccagtg aaatctagcg acggcattca aacttgggac ggcgtactac
     4441 aaggccagcg tttgcttacg atgtcctgct ccgataaaat tgctaggtgg aacatcgtcg
     4501 gtatccaggg ctccttgctc tcttcaatta tagagcccgt ctatctgcat tcaattgtct
     4561 tagggtcact cttgcatcct gaacatatgt accgagccgt ttgtgggaga atagaaaaga
     4621 gtatccaggg tctaccccca ccttaccatt tgaacaaacc cagactggca ttggtcacat
     4681 ccgcagaacc acgtaaccaa gccaaggccc cgaattttgg aataaactgg actataggtg
     4741 acacggaact agaggttgtg aactctctca caggcagaac cataggaggg caagtcagcc
     4801 gtataacgaa gcaggctttt ttcgtaaagt atggtttctt gatggctaat ttgcccggaa
     4861 tcctcgtccg aaaggtaacg accgattacg gtcagacaaa agcgaatgta aaggattacc
     4921 agatcgcgaa gttggaattg ttctcagcct tcaagaggga ggatcttgga tcatggctaa
     4981 agaaaccaat agagcaggac gagtttggat tggctgaatc tagaggaggt ggctcatctg
     5041 gcggaggtca gatctcgtac gcgtcccggg gcggtacctt cgacgacaag accgggcccg
     5101 gctcttctcc tcactcgacc tgcactagtc gatccaggcc tcccagcttt cgtccgtatc
     5161 atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgccca cacaccagaa
     5221 tcctactaag tttgagtatt atggcattgg aaaagctgtt ttcttctatc atttgttctg
     5281 cttgtaattt actgtgttct ttcagttttt gttttcggac atcaaaatgc aaatggatgg
     5341 ataagagtta ataaatgata tggtcctttt gttcattctc aaattattat tatctgttgt
     5401 ttttacttta atgggttgaa tttaagtaag aaaggaacta acagtgtgat attaaggtgc
     5461 aatgttagac atataaaaca gtctttcacc tctctttggt tatgtcttga attggtttgt
     5521 ttcttcactt atctgtgtaa tcaagtttac tatgagtcta tgatcaagta attatgcaat
     5581 caagttaagt acagtatagg ctttttgtgt cgagggggta ccgagtcgag gaattcactg
     5641 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5701 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5761 tcccaacagt tgcgcagcct gaatggcggg taccgagctc gaattcaatt cggcgttaat
     5821 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     5881 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     5941 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     6001 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     6061 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     6121 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     6181 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     6241 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     6301 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     6361 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     6421 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     6481 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     6541 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     6601 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     6661 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     6721 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     6781 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     6841 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     6901 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     6961 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     7021 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     7081 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     7141 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     7201 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     7261 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     7321 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     7381 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8101 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8161 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8221 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8281 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     8341 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     8401 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8461 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     8521 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     8581 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     8641 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     8701 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     8761 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     8821 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     8881 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     8941 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     9001 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     9061 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     9121 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     9181 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     9241 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     9301 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     9361 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     9421 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     9481 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     9541 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     9601 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     9661 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     9721 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     9781 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     9841 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     9901 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     9961 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    10021 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    10081 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    10141 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    10201 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    10261 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    10321 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    10381 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    10441 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    10501 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    10561 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    10621 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    10681 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    10741 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    10801 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    10861 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    10921 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    10981 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    11041 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    11101 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    11161 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    11221 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    11281 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    11341 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    11401 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    11461 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    11521 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    11581 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    11641 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    11701 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    11761 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    11821 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    11881 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    11941 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    12001 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    12061 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    12121 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    12181 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    12241 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttaaagta
    12301 ctttaaagta ctttaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12361 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
    12421 cgctcttttc tcttag
//
