LOCUS       sequence-403747-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of catalytically defective CSR-1a with 6xHis 
            and maltose binding protein tags for in vitro assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H
  TITLE     In vitro analyses of the production and activity of secondary small 
            interfering RNAs in C. elegans.
  JOURNAL   EMBO J. 2007 Dec 12. 26(24):5007-19.
  PUBMED    18007599
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2007 Dec 12. 26(24):5007-19."
COMMENT     Sequence Label: pHIT182
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             151..1251
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1225..1248
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4374..4391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4444..4462)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4458..4505
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4542..4997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4629..4648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4839..4860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5089..5904)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5089..5904)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5812..5831
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5896..5915
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6026..6614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6515..6534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6768..6785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6800..6939
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6865..6884)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7025..7047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7041..7232)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8041..9123)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9085..9104
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9124..9201)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9407..9426
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccataat atgaaaactg aagaaggtaa actggtaatc
      181 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
      241 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
      301 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
      361 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
      421 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
      481 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
      541 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
      601 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
      661 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
      721 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
      781 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
      841 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
      901 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
      961 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
     1021 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     1081 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     1141 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     1201 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tcatatggcg
     1261 gccgcgcagt cggataatgt cggtcgtggc cgtggaagcc gtggtggttc tcgtggaggt
     1321 agtggacgtg gtagaggacg tggaggatac ggagactcgt atcgtccgga tttagaaacg
     1381 agagaaaccc atgtaacgta tcaaggcaag gtaaagtatg aaggaggaga ccgtcatgat
     1441 ttcaactatg agaagacgcc aactggaagt tatcgtggct cgcatcgaga tggtcaggaa
     1501 ggatcgagag gcaaccgcgg atcattcaga ggtggacgag gaggttacga cagaagacgt
     1561 gatgatgaag aatcaaaagc agaaaatcgt tttggagggt acaatcgggg agcaaatggc
     1621 ggaaactatc gcgggagaac tggcgggagc ggcagaggtg gatcgaccca aaccactacc
     1681 gtggatccta aatataatgg attgatcttg gaaaattcag cagagccaaa gcttaaagga
     1741 cttaactctg acatgaatca gaagcaaaat ccaagacttg ccctgaacat cttcgggctt
     1801 gagctctctg agcgaacgat tttccggcat gttgtgcaaa tgaagctcat cgatcgtcag
     1861 cacaataagg agtatattct tacaacaatg agtgccagag gacgtggaaa ccgtgcaaca
     1921 aagcaaaaag ataactttat tcttcttgac atcctactca aacagtgggc cgcgaagaag
     1981 ggtcagcaga atcttcctgc attcgcgtac gacggtgcac agtctttatt cactttggag
     2041 ggaatcagct tgatggtcga tatcaagaaa gaagatgctc tcgaaatccc ggaactttcc
     2101 aattttctga aggattccat ctcgttcctc agcggcgatt tggagatctc ttgtgaaccg
     2161 gatctcgaga aaccgtcatt cgttcagacg gagcttaacg aatggagtga tccaagattc
     2221 tacgcttatt tggatattgt gacatcacag agtgccatta gaagcgaaag atatttgtcg
     2281 cagtcgaaag gtctctatgt ccacactcaa agcttggagg agcttcgcgt gaaatgggct
     2341 gttgcagcaa aaggaatcca caaaggatgc cgaatcgtcg gagcaaacgg acctcttcca
     2401 attctagaac tcgatcctca atcgactcag tactatgctt caatcccact cagtcagatg
     2461 ttgcagtacg catttcctcg tgattttccg ccaaaccgta tcgttaatcc aaatatgaaa
     2521 ctgcaaagag ccgtgaaact attgttgaag gatttaaaat gcaacccata ttacgatgac
     2581 aaacagattt gggctacgaa cactatcaca gtgtcggatg ttgactacaa cgcgccgaaa
     2641 gatccagaat tcagacaaaa gtatccaaat ctgaaattcc caatgcttcc tgcagttcaa
     2701 tgcggcactg gaccacacaa gcgcctcatg ccgctcgaat atttgaaggt tcttccttat
     2761 caatccattg accgccgtgt actcgaagag tttgagctta caccacgagc gaatgccccg
     2821 aacgaacgct ggtcaactct tcaaaaacac tacgatcaat ttggtttcaa cgatcaagtc
     2881 atgaaagatt ttggtgtcca aatctgcaat gatcctttca acaacgtcag tgagatcgac
     2941 ggtgaaagag tcctcgcgcc tagtgttgca tatgcagacc cggtacacgt ggatgatgag
     3001 aagcgtgact ggaaagcaca agacaaaaag tttgttacac ctgctacgat tgaccatttg
     3061 atgttcgttc tagtagcagg ttatactcga acttgggatg ctgattgcga tgcaaccaaa
     3121 tttgtcgcga aagcattcat gcagcgttgc aaggacaaag gaatgcacat tggcagttat
     3181 agcatggatc agcacaatgg cgaacgaggc agcgaaaatt tccttacttc ggtcttcaaa
     3241 aacttggtga ctcatccaaa ctaccgggat tcgtctttca ctccgttcgt cttgtttatt
     3301 tccgatgacg ttcctaacat tcatgagtgc ctcaaattcg aagagcgtat gagtgacatt
     3361 ccaacgcagc acgtacttct caaaaatgtc aaaaagatgc gtgacaacat cgaaaagaag
     3421 tctcaaggtg gaagaagagc atatgatttg actcttgaca atattgtgat gaaagccaac
     3481 attaaatgcg gtggacttaa ctacacagca gatattccaa gagatcttgc gtgctggaat
     3541 gaagtttcta ccttcgtaat tggaatggcc gttgctcatc ctgaccgaaa cgctgcgaga
     3601 gaaggaaatc catccaccgt cggactgtcc tgcaattctg ctgaaaaccc atactcgttc
     3661 attggagact tcttatacac cgatcctaga agagaggcga tccaggatga aattttgcga
     3721 aaattcactg accaaagcgt tcgaaacttt gccgaaattc gaggtttccc aaagaaagta
     3781 atcatcttcc gtgatggtgt ttcattcggt gaagagactg cggctctgaa ggaggtggaa
     3841 atcattgagc aaaccattaa gacagccgct aagtccatgg gccattctga ttatgctcca
     3901 aaagtattag ccattgttgt taaaaaacgt caccacactc gattctacgc caagggagga
     3961 catcacggga acatgccgat caatccgctt cccgatacat ctgttggtgg tgatattgcc
     4021 gaatatggaa aaagacaaat cttcatccaa gctttccggc cagtgcaagg tactgcaaag
     4081 gttccatcat tcttggttat tcgagatgat gaggaagttt cggacgaaca tgttgccaaa
     4141 atggtttgcg ccgtatgcag tcttcatcag cttgttaaca gtccaacatc catcccaact
     4201 ccagtttacg ttgctcatga gctcgccaag agaggaaccg ggctttacaa agcttatagg
     4261 ttcaagaatg gcgaactctt tgatgattgg gaaacgctca ccactcaact ctcctatagc
     4321 actctcgatc gactttcaaa agttcgagtt gtttagtcga caggcctgtc gagcaccacc
     4381 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     4441 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     4501 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
     4561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4681 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4741 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4801 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4861 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4921 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4981 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5041 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     5101 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     5161 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     5221 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     5281 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     5341 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     5401 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     5461 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     5521 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     5581 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     5641 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     5701 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     5761 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     5821 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga
     5881 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     5941 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6781 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     6841 cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6901 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     6961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7081 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     7141 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     7201 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     7261 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     7321 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     7381 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     7441 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     7501 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     7561 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     7621 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     7681 acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     7741 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     7801 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     7861 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     7921 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     7981 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     8041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8101 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     8161 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     8221 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     8281 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     8341 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     8401 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     8461 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     8521 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     8581 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     8641 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     8701 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     8761 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     8821 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     8881 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     8941 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     9001 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     9061 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     9121 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     9181 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     9241 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     9301 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     9361 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     9421 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     9481 agaggatcga gatctcgatc ccgcgaaat
//
