LOCUS       sequence-403779-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human LARP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label:  pLenti-C-Myc-DDK-IRES-Puro LARP7
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(428..532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(607..626)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        797..1176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1178..1376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1394..1574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1621..1746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2243..2476
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2661..2705
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3003..3120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3203..3582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3583..3786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3812..3831
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3812..3830
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5652..5681
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5700..5723
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5757..6330
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5780..6332
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5924..5941)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6151..6170
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6332..6931
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6332..6351)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6828..6848
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6961..6994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7050..7638
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7103..7123)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7521..7532)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7641..7657)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7642..7658)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8167..8347
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8409..8997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8489..8508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9074..9306,1..427))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
       61 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
      121 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
      181 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
      241 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      301 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      361 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      421 tctccatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      541 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
      601 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
      661 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      721 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      781 ggccagatat cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      841 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      901 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      961 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1021 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1081 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1141 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1201 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1261 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1321 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     1381 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1441 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1501 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1561 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1621 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1681 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1741 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1801 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1861 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1921 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1981 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     2041 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     2101 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2161 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2221 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2281 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2341 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2401 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2461 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2521 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2581 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2641 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2701 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2761 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2821 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2881 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2941 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     3001 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3061 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3121 cgggtttatt acagggacag cagagatcca gtttggttag taccgggccc gctctagaca
     3181 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     3241 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     3481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     3541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     3601 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     3661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
     3721 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     3781 agagctcgtt tagtgaaccg tcagaatttt gtaatacgac tcactatagg gcggccggga
     3841 attcgtcgac tggatccggt accgaggaga tctgccgccg cgatcgcatg gaaactgaaa
     3901 gtggaaatca ggaaaaggta atggaagaag aaagcactga aaagaaaaaa gaagttgaaa
     3961 aaaagaaacg gtcacgagtt aaacaggtgc ttgcagatat tgctaagcaa gtggacttct
     4021 ggtttgggga tgcaaatctt cacaaggata gatttcttcg agaacagata gaaaaatcta
     4081 gagatggata tgttgatata tcactacttg tgtcttttaa caaaatgaaa aaattgacta
     4141 ctgatgggaa gttaattgcc agagcgttga gaagttcagc tgttgtagag cttgatttgg
     4201 aaggcaccag aatccggagg aaaaaacctc tgggggaaag accaaaggat gaggatgaac
     4261 gcacagtgta tgtggagtta cttcccaaaa atgttaatca cagctggatt gaaagagtat
     4321 ttgggaaatg tggcaatgtt gtttatataa gtataccaca ttataagtct actggagatc
     4381 caaagggatt tgcgtttgtg gaatttgaaa caaaagaaca agcagcaaaa gcaattgagt
     4441 ttcttaacaa cccaccagaa gaagcaccaa gaaaacctgg catatttcct aaaacagtga
     4501 aaaataagcc cattccagcc ttaagagttg tggaagagaa gaaaaagaaa aagaagaaga
     4561 aaggccgaat gaaaaaggaa gacaatatcc aagccaaaga agaaaacatg gacacaagca
     4621 acaccagcat cagtaaaatg aaaagatcca gacccacatc tgagggctct gacattgagt
     4681 ccactgaacc ccaaaagcag tgctcaaaga aaaagaaaaa acgggacaga gttgaagcat
     4741 ctagcttacc tgaagtcaga acagggaaga ggaagagaag cagctctgaa gatgcagaat
     4801 ccctagctcc ccgatcaaaa gtaaagaaaa ttattcagaa agacatcatt aaggaagcat
     4861 cagaagcttc caaggaaaat agagatatag aaatctctac tgaagaggaa aaggatactg
     4921 gagatctaaa agatagctct ctcttgaaaa caaaaaggaa acataagaaa aaacataaag
     4981 agagacataa aatgggagaa gaagttatac cattaagagt gctatcaaag agcgaatgga
     5041 tggatttgaa aaaagagtat ttagcgctac aaaaagctag catggcttct ttaaaaaaaa
     5101 caatatccca aataaaatca gagtcagaaa tggaaacaga cagtggagta cctcaaaaca
     5161 ctggaatgaa aaatgaaaaa acagccaaca gggaagagtg tcgcacccag gagaaagtta
     5221 atgcaacagg accacagttc gtgagtggag tgattgtgaa gatcattagc acagagcctc
     5281 tacctggcag gaaacaagtc cgggatactt tggcagcaat ctcagaagtt ctttatgttg
     5341 atttgctaga aggggataca gaatgccatg ctagatttaa aactcctgag gatgctcaag
     5401 cagtaataaa tgcctataca gaaattaaca agaaacactg ctggaaactc gagatccttt
     5461 ctggtgatca cgaacaaagg tattggcaga agattttggt tgatagacag gcaaaactta
     5521 atcagcctcg ggaaaagaaa agaggcactg aaaagttaat caccaaagct gaaaagatta
     5581 ggctggcaaa gactcaacaa gcgagtaaac atataagatt ttctgaatat gatacgcgta
     5641 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     5701 attacaagga tgacgacgat aaggtttaaa cctaggcgta gcggccgcaa attccgcccc
     5761 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5821 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5881 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5941 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     6001 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     6061 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     6121 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     6181 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     6241 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     6301 gtggttttcc tttgaaaaac acgatgataa tatgaccgag tacaagccca cggtgcgcct
     6361 cgccacccgc gacgacgtcc cccgggcagt acgcaccctc gccgccgcgt tcgccgacta
     6421 ccccgccacg cgccacaccg tcgatccaga ccgccacatc gagcgggtca ccgagctgca
     6481 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6541 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6601 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6661 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6721 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6781 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6841 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6901 ctggtgcatg acccgcaagc ccggtgcctg atgtacaagt aggattcgtc gagggaccta
     6961 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
     7021 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     7081 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7141 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7201 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7261 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7321 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7381 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7441 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7501 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7561 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7621 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     7681 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7741 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7801 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7861 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7921 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     7981 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     8041 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     8101 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     8161 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8221 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8281 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8341 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9061 attttggtca tgattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     9121 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     9181 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     9241 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     9301 cgaaaa
//
