LOCUS       sequence-403786-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p62 in a doxycycline-dependent manner in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, 
            Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H,
            Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M
  TITLE     Integrated proteomics identifies p62-dependent selective autophagy 
            of the supramolecular vault complex.
  JOURNAL   Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015.
  PUBMED    37192622
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015."
COMMENT     Sequence Label: pRetroX-TetOne-Puro-p62
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        177..556
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    987..1344
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1755..1777
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1793..1809
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2147..2163
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     polyA_signal    2311..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3979..4346)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4104..4122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4140..4158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4176..4194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4212..4230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4248..4266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4284..4302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4320..4338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4363..4873
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4792..4810
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4892..5638
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        5656..5985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5836..5971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5994..6593
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5994..6013)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6610..7013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    8033..8154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8177..8194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8177..8193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8186..8208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8402..8421)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8521..8543
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8581..8599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8667..8771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8772..8840,8841..9632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8990..9009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9803..10391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10292..10311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10545..10562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10679..10700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10715..10720,10721..10738,10739..10745)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10753..10769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10758..10780
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(10777..10791,1..2)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10777..10791,1..2)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 accatgatta cgccaagcta gctttgctct taggagtttc ctaatacatc ccaaactcaa
       61 atatataaag catttgactt gttctatgcc ctaggagctt ggcccattgc atacgttgta
      121 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca
      181 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      241 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      301 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      361 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      421 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      481 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      541 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      601 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      661 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      721 catctagagc gcttctgctc cccgagctct ataaaagagc ccacaacccc tcactcgggg
      781 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
      841 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
      901 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag
      961 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat
     1021 tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc
     1081 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
     1141 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcct aaaatcccga
     1201 tcgtttagga ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga
     1261 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     1321 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1381 tctgtatttg tctgaaaatg agggcccggg ctagcctgtt accactccct taagtttgac
     1441 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1501 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1561 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1621 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1681 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1741 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1801 agccctcact ccttctctag gcgcccccat atggccatat gagatcctat atggggcacc
     1861 cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct
     1921 ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc
     1981 agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac
     2041 agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac
     2101 agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc
     2161 cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagatcat aatcagccat
     2221 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2281 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2341 aaataaggca atagcatcac aaatttcaca aataaggcat ttttttcact gcattctagt
     2401 tttggtttgt ccaaactcat caatgtatct tatcatgtct ggatctcaaa tccctcggaa
     2461 gctgcgcctg tcttaggttg gagtgataca tttttatcac ttttacccgt ctttggatta
     2521 ggcagtagct ctgacggccc tcctgtctta ggttagtgaa aaatgtcact ctcttacccg
     2581 tcattggctg tccagcttag ctcgcagggg aggtggtctg gatccgccgg caccggtgta
     2641 tacgggaatt tcacaacggc gggggatgct ttgaatactg gatggtgtcc agagccgctc
     2701 cgatgtcata gttcttggtc tgcaggagcc tggtgagcca gccgccttca tcagagaagc
     2761 ccatggacag catctgggag agggactcaa tcagccgcgg gtcagcctct ggcgggagat
     2821 gtgggtacaa ggcagcttcc ttcagccctg tgggtccctc ctgggagggg tccagagagc
     2881 ttggcccttc ggattctggc atctgtaggg actggagttc acctgtagac gggtccactt
     2941 cttttgaaga cagatgggtc cagtcatcat ctcctcctga gcaattgtct gattccattt
     3001 gttcctcagg gcgcccctcg gactccaagg cgatctttct catctgctcc gccagagact
     3061 gcgtggcgcc ctcaacattc ccacccggct tgctgggatc agagcagcag ctgcttggct
     3121 gtgagctgct cttctcctct gtgctggaac tctctggaga gacgggggtc aggcggcttc
     3181 ttttccctcc gtgctccaca tcgatatcaa cttcaatgcc cagagggcta agggcagctg
     3241 ccacactctc cccaacgttc ttcaggaaat tcacactcgg atcctccgat ggaccagaag
     3301 ctgattctgc cgtggggcca gggcgggcct cccctgcacg aggaggacgt gggctccagt
     3361 ttcctggtgg acccatttcc catcctggcc acccgaagtg tccgtgtttc accttccgga
     3421 gccagcggct gtgcgagaag ccctcagaca ggtgcccgaa ggggctgggg aatgcgagct
     3481 tggtgtgccc ccggtgcaag ccctttccct cgcagacgct acacaagtcg tagtctgggc
     3541 agacgctgca cttgtagcgg gttcctacca caggcccatt gcagccatcg cagatcacat
     3601 tggggtgcac catgttgcgg ggcgcctcct gagcacacgg tgggcggtgg tcccgccggc
     3661 actctttttt ctctttaatg tagattcgga agatgtcatc cttcacgtag gacatggcca
     3721 ttgtcaattc ctcgtcactg gaaaaggcaa ccaagtcccc gtcctcatcg cggtagtgcg
     3781 cctggaagcc gccaggccgc agcgcgggga acagggcggc cacccggctc agcagccgct
     3841 cgcagggtcc cggacccgcc gcagcctcgg cttccgcctc aggctcgggg ctgcagcaga
     3901 agctgaagcg gcgaatctcg cgcgccgcgt cctccttgcc cagaaggtag gccttcacgg
     3961 tgagcgacgc cataattctt tacgagggta ggaagtggta cggaaagttg gtataagaca
     4021 aaagtgttgt ggaattgaag tttactcaaa aaatcagcac tcttttatag gcgccctggt
     4081 ttacataagc aaagcttata cgttctctat cactgatagg gagtaaactg gatatacgtt
     4141 ctctatcact gatagggagt aaactgtaga tacgttctct atcactgata gggagtaaac
     4201 tggtcatacg ttctctatca ctgataggga gtaaactcct tatacgttct ctatcactga
     4261 tagggagtaa agtctgcata cgttctctat cactgatagg gagtaaactc ttcatacgtt
     4321 ctctatcact gatagggagt aaactcgagg tgataattcc acggggttgg ggttgcgcct
     4381 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4441 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4501 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4561 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4621 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4681 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4741 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4801 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4861 cctctctccc cagggggatc atcgaattac catgtctaga ctggacaaga gcaaagtcat
     4921 aaactctgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
     4981 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     5041 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact cctgccccct
     5101 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     5161 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     5221 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     5281 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     5341 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     5401 gaaacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     5461 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     5521 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     5581 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact
     5641 aagtaagaat tgaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5701 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5761 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5821 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5881 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5941 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaacgcg accatgaccg
     6001 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     6061 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     6121 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6181 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6241 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6301 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6361 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6421 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6481 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6541 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgta
     6601 attcctgtta atcaacctct ggattacaaa atttgtgaaa gattgactga tattcttaac
     6661 tatgttgctc cttttacgct gtgtggatat gctgctttaa tgcctctgta tcatgctatt
     6721 gcttcccgta cggctttcgt tttctcctcc ttgtataaat cctggttgct gtctctttat
     6781 gaggagttgt ggcccgttgt ccgtcaacgt ggcgtggtgt gctctgtgtt tgctgacgca
     6841 acccccactg gctggggcat tgccaccacc tgtcaactcc tttctgggac tttcgctttc
     6901 cccctcccta tcgccacggc agaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6961 gctaggttgc tgggcactga taattccgtg gtgttgtcgg ggaagctgac gtcgagatct
     7021 tcctgcggag atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
     7081 gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg
     7141 gggaatgaaa gaccccacct gtaggtttgg caagctagct tctcgcttct gttcgcgcgc
     7201 ttctgctccc agacatcaac aaaagagccc acaacccctc actcggggcg ccagtcctcc
     7261 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     7321 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     7381 gggtctttca cgcgtatgcc gcatagttaa gccaccgcgg tggcggtctg gaccacgccg
     7441 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     7501 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     7561 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7621 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7681 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7741 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7801 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatggctcc
     7861 gaccgaagcc acccggggcg gccccgccga ccccgcaccc gcccccgagg cccaccgact
     7921 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     7981 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     8041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgg
     8161 cgcgtcgaat taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8221 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8281 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     8341 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     8401 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8461 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8521 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     8581 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8641 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8701 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8761 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8821 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8881 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8941 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9001 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9061 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9121 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9181 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9241 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9301 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9361 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9421 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9481 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9541 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9601 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9661 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9721 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10441 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10501 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10561 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10621 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10681 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10741 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat g
//
