LOCUS       sequence-403787-       10635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p62 deltaUBA in a doxycycline-dependent manner in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, 
            Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H,
            Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M
  TITLE     Integrated proteomics identifies p62-dependent selective autophagy 
            of the supramolecular vault complex.
  JOURNAL   Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015.
  PUBMED    37192622
REFERENCE   2  (bases 1 to 10635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015."
COMMENT     Sequence Label: pRetroX-TetOne-Puro-p62 deltaUBA
FEATURES             Location/Qualifiers
     source          1..10635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        159..538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    969..1326
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1737..1759
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1775..1791
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2129..2145
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     polyA_signal    2293..2427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3805..4172)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3930..3948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3966..3984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4002..4020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4038..4056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4074..4092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4110..4128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4146..4164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4189..4699
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4618..4636
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4718..5464
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        5482..5811
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5662..5797
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5820..6419
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5820..5839)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6436..6839
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    7859..7980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8003..8020)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8003..8019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8012..8034)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8228..8247)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8347..8369
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8407..8425)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8493..8597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8598..8666,8667..9458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8816..8835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9629..10217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10118..10137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10371..10388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10505..10526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10541..10546,10547..10564,10565..10571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10579..10595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10584..10606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10603..10619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10603..10619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta
      121 catttatatt ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcatctaga gcgcttctgc
      721 tccccgagct ctataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
      781 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
      841 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
      901 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
      961 cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact
     1021 gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt
     1081 ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc cagggacttc
     1141 gggggccgtt tttgtggccc gacctgagtc ctaaaatccc gatcgtttag gactctttgg
     1201 tgcacccccc ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt
     1261 cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt
     1321 ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1381 tgagggcccg ggctagcctg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1441 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1501 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1561 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1621 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1681 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1741 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1801 aggcgccccc atatggccat atgagatcct atatggggca cccccgcccc ttgtaaactt
     1861 ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc
     1921 tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact
     1981 ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca
     2041 ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc
     2101 caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc
     2161 cgaccccggg ggtggaccat cctctagatc ataatcagcc ataccacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg caatagcatc
     2341 acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt gtccaaactc
     2401 atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc tgtcttaggt
     2461 tggagtgata catttttatc acttttaccc gtctttggat taggcagtag ctctgacggc
     2521 cctcctgtct taggttagtg aaaaatgtca ctctcttacc cgtcattggc tgtccagctt
     2581 agctcgcagg ggaggtggtc tggatccgcc ggcaccggtg tatacgggaa tttcatggcg
     2641 ggagatgtgg gtacaaggca gcttccttca gccctgtggg tccctcctgg gaggggtcca
     2701 gagagcttgg cccttcggat tctggcatct gtagggactg gagttcacct gtagacgggt
     2761 ccacttcttt tgaagacaga tgggtccagt catcatctcc tcctgagcaa ttgtctgatt
     2821 ccatttgttc ctcagggcgc ccctcggact ccaaggcgat ctttctcatc tgctccgcca
     2881 gagactgcgt ggcgccctca acattcccac ccggcttgct gggatcagag cagcagctgc
     2941 ttggctgtga gctgctcttc tcctctgtgc tggaactctc tggagagacg ggggtcaggc
     3001 ggcttctttt ccctccgtgc tccacatcga tatcaacttc aatgcccaga gggctaaggg
     3061 cagctgccac actctcccca acgttcttca ggaaattcac actcggatcc tccgatggac
     3121 cagaagctga ttctgccgtg gggccagggc gggcctcccc tgcacgagga ggacgtgggc
     3181 tccagtttcc tggtggaccc atttcccatc ctggccaccc gaagtgtccg tgtttcacct
     3241 tccggagcca gcggctgtgc gagaagccct cagacaggtg cccgaagggg ctggggaatg
     3301 cgagcttggt gtgcccccgg tgcaagccct ttccctcgca gacgctacac aagtcgtagt
     3361 ctgggcagac gctgcacttg tagcgggttc ctaccacagg cccattgcag ccatcgcaga
     3421 tcacattggg gtgcaccatg ttgcggggcg cctcctgagc acacggtggg cggtggtccc
     3481 gccggcactc ttttttctct ttaatgtaga ttcggaagat gtcatccttc acgtaggaca
     3541 tggccattgt caattcctcg tcactggaaa aggcaaccaa gtccccgtcc tcatcgcggt
     3601 agtgcgcctg gaagccgcca ggccgcagcg cggggaacag ggcggccacc cggctcagca
     3661 gccgctcgca gggtcccgga cccgccgcag cctcggcttc cgcctcaggc tcggggctgc
     3721 agcagaagct gaagcggcga atctcgcgcg ccgcgtcctc cttgcccaga aggtaggcct
     3781 tcacggtgag cgacgccata attctttacg agggtaggaa gtggtacgga aagttggtat
     3841 aagacaaaag tgttgtggaa ttgaagttta ctcaaaaaat cagcactctt ttataggcgc
     3901 cctggtttac ataagcaaag cttatacgtt ctctatcact gatagggagt aaactggata
     3961 tacgttctct atcactgata gggagtaaac tgtagatacg ttctctatca ctgataggga
     4021 gtaaactggt catacgttct ctatcactga tagggagtaa actccttata cgttctctat
     4081 cactgatagg gagtaaagtc tgcatacgtt ctctatcact gatagggagt aaactcttca
     4141 tacgttctct atcactgata gggagtaaac tcgaggtgat aattccacgg ggttggggtt
     4201 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     4261 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     4321 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     4381 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     4441 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     4501 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     4561 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     4621 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     4681 caccgacctc tctccccagg gggatcatcg aattaccatg tctagactgg acaagagcaa
     4741 agtcataaac tctgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag
     4801 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     4861 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccactcctg
     4921 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg
     4981 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     5041 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     5101 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     5161 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     5221 acttctgaaa caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     5281 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     5341 gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
     5401 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
     5461 gtaactaagt aagaattgaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5641 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5761 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aacgcgacca
     5821 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     5881 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     5941 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6001 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6061 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6121 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6181 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6241 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6301 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6361 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     6421 cgcgtaattc ctgttaatca acctctggat tacaaaattt gtgaaagatt gactgatatt
     6481 cttaactatg ttgctccttt tacgctgtgt ggatatgctg ctttaatgcc tctgtatcat
     6541 gctattgctt cccgtacggc tttcgttttc tcctccttgt ataaatcctg gttgctgtct
     6601 ctttatgagg agttgtggcc cgttgtccgt caacgtggcg tggtgtgctc tgtgtttgct
     6661 gacgcaaccc ccactggctg gggcattgcc accacctgtc aactcctttc tgggactttc
     6721 gctttccccc tccctatcgc cacggcagaa ctcatcgccg cctgccttgc ccgctgctgg
     6781 acaggggcta ggttgctggg cactgataat tccgtggtgt tgtcggggaa gctgacgtcg
     6841 agatcttcct gcggagatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
     6901 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
     6961 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttctc gcttctgttc
     7021 gcgcgcttct gctcccagac atcaacaaaa gagcccacaa cccctcactc ggggcgccag
     7081 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     7141 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     7201 cagcgggggt ctttcacgcg tatgccgcat agttaagcca ccgcggtggc ggtctggacc
     7261 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7321 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7381 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7441 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7501 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7561 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7621 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     7681 ggctccgacc gaagccaccc ggggcggccc cgccgacccc gcacccgccc ccgaggccca
     7741 ccgactctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7801 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7861 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7921 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7981 tcatggcgcg tcgaattaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8041 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8101 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     8161 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     8221 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     8281 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8341 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     8401 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8461 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8521 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8581 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8641 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8701 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8761 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8821 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8881 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8941 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9001 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9061 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9121 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9181 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9241 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9301 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9361 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9421 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10321 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10381 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10441 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10501 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10561 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10621 atgattacgc caagc
//
