LOCUS       sequence-403790-        6113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for bacterial expression of human protein ELMOD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6113)
  TITLE     Kahn Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX4-ELMOD3
FEATURES             Location/Qualifiers
     source          1..6113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             198..725
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(218..235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(218..234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(227..249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(827..832,833..848,849..855)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    863..879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             902..1555
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1513..1535
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1562..1579
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2796..2818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2963..2981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3049..3153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3154..3222,3223..4014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3372..3391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4185..4773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4674..4693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4938..4955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5028..5105
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(5106..6113,1..75)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5125..5144)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc
      241 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      301 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      361 tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc
      421 tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg
      481 gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg
      541 ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc
      601 tacaggaagg ccagacgcga attatttttg atggcgttgg aattagctta tcgactgcac
      661 ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt
      721 cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt
      781 tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca
      841 tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagtatt
      901 catgtcccct atactaggtt attggaaaat taagggcctt gtgcaaccca ctcgacttct
      961 tttggaatat cttgaagaaa aatatgaaga gcatttgtat gagcgcgatg aaggtgataa
     1021 atggcgaaac aaaaagtttg aattgggttt ggagtttccc aatcttcctt attatattga
     1081 tggtgatgtt aaattaacac agtctatggc catcatacgt tatatagctg acaagcacaa
     1141 catgttgggt ggttgtccaa aagagcgtgc agagatttca atgcttgaag gagcggtttt
     1201 ggatattaga tacggtgttt cgagaattgc atatagtaaa gactttgaaa ctctcaaagt
     1261 tgattttctt agcaagctac ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa
     1321 aacatattta aatggtgatc atgtaaccca tcctgacttc atgttgtatg acgctcttga
     1381 tgttgtttta tacatggacc caatgtgcct ggatgcgttc ccaaaattag tttgttttaa
     1441 aaaacgtatt gaagctatcc cacaaattga taagtacttg aaatccagca agtatatagc
     1501 atggcctttg cagggctggc aagccacgtt tggtggtggc gaccatcctc caaaatcgga
     1561 tctggttccg cgtggatcca tgaatgaaaa atcttgctct ttccatagta aagaagaatt
     1621 aagagatgga cagggtgaaa gattgtctgc tggatattct ccatcatatg acaaggacaa
     1681 gagtgttctg gctttcagag gaatccctat ctcagagttg aagaaccatg gcattctcca
     1741 ggctctgacc acagaagctt atgaatggga gccacgtgtt gtgagtacag aggtggtcag
     1801 agcccaagaa gaatgggaag ctgtggacac catccagcca gagacaggga gccaagctag
     1861 ctcagagcag cctgggcagc taatctcctt cagtgaggcc ctgcagcact tccagactgt
     1921 ggacctttcc cccttcaaga aaagaatcca gccaactatt cgaaggactg ggctcgccgc
     1981 cctccgacac tacctcttcg ggcctccaaa gctccaccag cgccttcggg aagaaaggga
     2041 cttggtcctg accattgctc agtgtggcct ggatagccaa gacccagtgc atggccgagt
     2101 cctccagacc atctataaga agctgaccgg ctccaagttt gactgtgccc ttcatggaaa
     2161 ccactgggag gacctgggct ttcagggagc gaatccagcc acagacctga gaggcgcagg
     2221 cttccttgcc ctcctgcatc tgctctacct ggtgatggac tcaaagacct tgccgatggc
     2281 gcaggagatt ttccgcctgt ctcgtcacca catccagcaa ttccctttct gtttgatgtc
     2341 cgtgaacatc acccacattg ccatccaggc cttgagagag gagtgtctct ccagagagtg
     2401 taatcggcag cagaaggtca tccccgtggt gaacagcttc tatgccgcca cattcctcca
     2461 cctcgcacat gtctggagga cacagcggaa gaccatctca gactcgggct ttgtcctcaa
     2521 agagttggaa gtattggcca agaagagccc acggcggctg ctcaagaccc tggagctgta
     2581 cttggccagg gtgtcaaagg gacaggcctc cttgttggga gcacagaagt gctatgggcc
     2641 agaagcccct cccttcaagg atctcacctt cacaggtgag agtgacctgc agtctcactc
     2701 atccgaaggc gtatggctga tctgagtcga ctcgagcggc cgcatcgtga ctgactgacg
     2761 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2821 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2881 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     2941 tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3001 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3061 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3361 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     3421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3661 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     3721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3841 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4081 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4321 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4801 ttcctggcct tttgctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     4981 attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat
     5041 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
     5101 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     5161 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     5221 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     5281 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     5341 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     5401 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     5461 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     5521 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     5581 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     5641 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     5701 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     5761 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     5821 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     5881 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     5941 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     6001 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     6061 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa acc
//
