LOCUS       sequence-403807-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified CROPseq vector for efficient CRISPR screens in 
            hematopoiesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Martin-Rufino JD, Castano N, Pang M, Grody EI, Joubran S, Caulier A,
            Wahlster L, Li T, Qiu X, Riera-Escandell AM, Newby GA, Al'Khafaji A,
            Chaudhary S, Black S, Weng C, Munson G, Liu DR, Wlodarski MW, Sims 
            K, Oakley JH, Fasano RM, Xavier RJ, Lander ES, Klein DE, Sankaran VG
  TITLE     Massively parallel base editing to map variant effects in human 
            hematopoiesis.
  JOURNAL   Cell. 2023 Apr 27:S0092-8674(23)00332-X. doi: 
            10.1016/j.cell.2023.03.035.
  PUBMED    37137305
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Apr 27:S0092-8674(23)00332-X. doi: 10.1016/j.cell.2023.03.035."
COMMENT     Sequence Label: pCROPseq-VEX-optimized-scaffold
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      412..1000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     901..920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1154..1171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1288..1309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1324..1329,1330..1347,1348..1354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1362..1378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1367..1389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1386..1402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1386..1402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1421..1441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1423..1441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1469..1695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1696..1876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1923..2048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2541..2774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2959..3003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3184..3301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3440..4618
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3671..4609
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4566..4586
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4625..4634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4631..4633,4634..4636,4637..5350)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4676..4697)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4937..4956)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5284..5305
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5366..5954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5419..5439)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5837..5848)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6110..6358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6110..6130
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6281..6300
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             8378..8558
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8636..8757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8673..8692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8727..8746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8797..8932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8952..8971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8953..8971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8981..8998)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8981..8997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8990..9012)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9139..9594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9226..9245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9436..9457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9620..9724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9725..9793,9794..10344,1..241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9943..9962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
       61 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      541 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1021 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1081 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1141 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1261 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1321 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     1441 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     1501 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     1561 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     1621 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     1681 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     1741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     1801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1861 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1921 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1981 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2041 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2101 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     2161 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     2221 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     2281 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     2341 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     2401 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     2461 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     2521 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     2581 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     2641 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     2701 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     2761 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     2821 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     2881 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     2941 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3001 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3061 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3121 aggcagggat attcaccatt atcgtttcag aatcttgaga caaatggcag tattcatcca
     3181 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     3241 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     3301 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt
     3361 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     3421 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     3481 agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg
     3541 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3601 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3661 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3721 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3781 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3841 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3901 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3961 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     4021 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     4081 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     4141 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     4201 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     4261 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     4321 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     4381 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     4441 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     4501 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4561 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg
     4621 tacggccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4681 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4741 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4801 ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc gctaccccga
     4861 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4921 caccatcagc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4981 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5041 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca cggccgacaa
     5101 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5161 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5221 cgacaaccac tacctgttca tccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5281 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct
     5341 gtacaagtaa acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     5401 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5461 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5521 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5581 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5641 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5701 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5761 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5821 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5881 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5941 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     6001 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6061 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
     6121 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     6181 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     6241 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     6301 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
     6361 agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt
     6421 ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag aatttttatg
     6481 ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac
     6541 tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag
     6601 ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa
     6661 ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg
     6721 attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac
     6781 aagctttcat gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat
     6841 gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt
     6901 cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt
     6961 cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat
     7021 ttgttattcc tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat
     7081 taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag
     7141 ctttcgtagc atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt
     7201 ctcctatata ttcattttta attttaattc tttaataatt tcgtctactt taactttagc
     7261 gttttgaaca gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact
     7321 tgggcgaaca gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg
     7381 catattatac attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg
     7441 agttaagggt gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc
     7501 tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc
     7561 ttctaaatag tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat
     7621 taatatagaa gcttgtattc catctttatc tctagctgag tcatcaatta catatccata
     7681 actttcttca taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct
     7741 acccattcat ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc
     7801 gattctatca cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat
     7861 gctatttaag cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt
     7921 tccatctaat cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc
     7981 tgggtcattc ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt
     8041 tcatgcagga tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa
     8101 tgcatgctct tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa
     8161 tttagttcct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     8221 ctcttttttg tacgtctctg tttcagagct atgctggaaa cagcatagca agttgaaata
     8281 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc
     8341 gtaactagat cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat
     8401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     8581 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     8641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8761 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8821 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8881 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     8941 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     9001 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9061 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9121 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     9181 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     9241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     9361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9481 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9541 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     9601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10261 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10321 agcttcccgg caacaattaa taga
//
