LOCUS       sequence-403812-        4294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A)
            used for microinjection into nematode.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired 
            silencing of meiotic chromosomes.
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. 
            Epub 2023 Apr 20.
  PUBMED    37078421
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-1"; volume: "42(11):e105002. doi"; pages: " 
            10.15252/embj.2020105002. Epub 2023 Apr 20"
COMMENT     Sequence Label: pHIT239
FEATURES             Location/Qualifiers
     source          1..4294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1357..1375)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1371..1418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1447..1464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1618..2206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1698..1717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2377..3168,3169..3237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3000..3019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3238..3342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3410..3428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3466..3488)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3588..3607
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3676..4131)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3813..3834)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4025..4044
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4257..4279
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4271..4288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4272..4288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggt ttaattaccc aagtttgagg cctcaccatg tcgagtttcg
       61 tgccgaataa agagcaaacg cggacagtat taattttctg ttttcatttg aagaaaacag
      121 ctgcggaatc gcaccgaatg cttgttgaag cctttggcga acaagtacca actgtgaaaa
      181 cgtgtgaacg gtggtttcaa cgcttcaaaa gtggtgattt tgacgtcgac gacaaagagc
      241 acggaaaacc gccaaaaagg tacgaagacg ccgaactgca agcattattg gatgaagacg
      301 atgctcaaac gcaaaaacaa ctcgcagagc agttggaagt aagtcaacaa gcagtttcca
      361 atcgcttgcg agagatggga aagattcaga aggtcggtag atgggtgcca catgagttga
      421 acgagaggca gatggagagg cgcaaaaaca catgcgaaat tttgctttca cgatacaaaa
      481 ggaagtcgtt tttgcatcgt atcgttactg gagatgaaaa atggatcttt tttgttaatc
      541 ctaaacgtaa aaagtcatac gttgatcctg gacaaccggc cacatcgact gctcgaccga
      601 atcgctttgg caagaagacg atgctctgtg tttggtggga tcagagcggt gtcatttact
      661 atgagctctt gaaacccggc gaaacggtga atacggcacg ctaccaacaa caattgatca
      721 atttgaaccg tgcgcttcag agaaaacgac cggaatatca aaaaagacaa cacagggtca
      781 tttttctcca tgacaacgct ccatcacata cggcaagggc ggttcgcgac acgttggaaa
      841 cactcaattg ggaagtgctt ccgcatgcgg cttactcacc agacctggcc ccatccgatt
      901 accacctatt cgcttcgatg ggacacgcac tcgctgagca gcgcttcgat tcttacgaaa
      961 gtgtgaaaaa atggctcgat gaatggttcg ccgcaaaaga cgatgagttc tactggcgtg
     1021 gaatccacaa attgcccgag agatgggaaa aatgtgtcgc gagcgacggc aaatactttg
     1081 aatagctagc atgtatctgc atatccttca aatgttgtcc atattgtatc agtaaattat
     1141 aatgccccct ttttatatat tctccttatc atgtaaatgt tctattgatt ttttgctggt
     1201 gtaaaacgtt tttatagtaa atcctctcca cagtgaattt gttaaattgt atcaaaacaa
     1261 gacattgatt aaatttgttc tagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1321 aaaaaaaaaa aaaaaaaaaa aaaagagacc actagtccgc tgagcaataa ctagcataac
     1381 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     1441 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2041 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3361 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3421 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     3481 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     3541 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     3601 gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3661 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3721 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3781 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3841 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3901 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3961 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4021 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4081 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     4141 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4201 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     4261 gtcacgacgt tgtaaaacga cggccagtga attg
//
