LOCUS       sequence-403815-        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of C04F12.1 with 6xHis and maltose binding 
            protein tags for in vitro assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired 
            silencing of meiotic chromosomes.
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. 
            Epub 2023 Apr 20.
  PUBMED    37078421
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-1"; volume: "42(11):e105002. doi"; pages: " 
            10.15252/embj.2020105002. Epub 2023 Apr 20"
COMMENT     Sequence Label: pHIT203
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             151..1251
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1225..1248
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3127..3138
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             4049..4066
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4119..4137)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4133..4180
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4217..4672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4304..4323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4514..4535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4764..5579)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4764..5579)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5487..5506
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5571..5590
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5701..6289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6190..6209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6443..6460
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6475..6614
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6540..6559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6700..6722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6716..6907)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7716..8798)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(8799..8876)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9082..9101
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccataat atgaaaactg aagaaggtaa actggtaatc
      181 tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa
      241 gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag
      301 gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc
      361 tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg
      421 tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct
      481 gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg
      541 gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc
      601 aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc
      661 aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa
      721 gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat
      781 tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg
      841 tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc
      901 ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc
      961 agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt
     1021 ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa
     1081 gagttggcga aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc
     1141 atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac
     1201 gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tcatatggcg
     1261 gccgcaaagg acgagcaatc agctccagta gttccaactc ctgaattgtc tcgtgtctct
     1321 gtttcctcca atcctccgac tccttctgca attgaaaaac ctgtccctct tcctgttcca
     1381 tcgaaaccat tgttcgagta cgaacctcca gcacaactgg aagctttggc taagaataag
     1441 cctgtgaaag tgacaaccaa ctcatacgaa cttgagttga agccaactcc tgtttttcgt
     1501 tacgacgtca gtgttctgaa gagctttgac gattcaacca gggatccaat tgagcttgct
     1561 ggctcgaaag gcggcgatcg tcagcggcaa gctgacttga cggaaatcgt gaaagttgct
     1621 ctcacattgg aaggccttgg aaatctgaac atcttctacg atggcgcagc gatgcttttc
     1681 acaactcaga aattcgaaac aaaaacggaa aagcagcaag caattttcat gccaattccg
     1741 tctgatcatc caaagctctc aacatctctc aaagaaagcc aattcggaag agcttccgga
     1801 aagttccacg tgtccatcga gttgaactcg aaacaatcgg aactaaatac aatcgatctt
     1861 ctcaatgaac gtctggattc agcagcttgt ccagtcactc agatggctca aatcgctctg
     1921 agtgcagagg ccaaacaaaa tggcttcatc attttggatg gaggacatga actcttcgat
     1981 cagaagaata caagaagaac aattggaaaa gatggagttg aggatatgga tggagtggct
     2041 gccagtataa aaatggctca aggaaataag aaaactggat ctgcacactt ggtcatggac
     2101 tacaaaaaga agcagttctt caagactggt ccattgaagg atttgataaa aggaatcaat
     2161 ccaaatcagc tccgttcata tctcaaaggt cttcgtgtcc acactacata ctctcatcaa
     2221 tcaatcttca ttgatggaat ttccaatgtc ccaatcggag aaattaaact tcccgatggt
     2281 actcctcttc ttgatgattg tgctcgagtt tccggaaagc ccaagagctt ttttgatgct
     2341 tccaagcctg cggttcaagt taaccagtac aacaaacggg aaggtagacg tatggtttat
     2401 agcttcccta tcgagaattt actggtaaaa ccaaaccaga aactgactca gaaccatgga
     2461 gaaccaccga gaagcatcaa gccggagctg agatttgatc tcataagaaa ggttggagag
     2521 tctgcaaagc ttctctctcc aaatcagaca ctgaaatcaa ttggtattgc gatcaatcca
     2581 gaaccgatcg ttgttgaagc tatgactgtg ccaaagccaa ccattctata caaagacgga
     2641 gcatttactt ctccagatct gttaaacaga acatcatggg acgttcaagc acgtaaccac
     2701 caatctctaa aaggcggatt tgtggaaccc atgaagatca acaagatctt gattctgtat
     2761 aatagtcaat cctacagtca agcaagtcca aaagaagcag ttgtcgaact gcaatcaatt
     2821 ctcagcaaaa aagccaaaga tgttggaatg acaattgggc aaattgatgt ggaagatctc
     2881 aatgatccgg atatcatcgg agcaattgaa aacaaaatga ccattctaaa aacgagccaa
     2941 gtcaagccaa ttgtgatcta cgccgatcat actagtgagg gtacccacag cactttgaag
     3001 cttcaggaac gattgtgtga ggtcataact caacaagtcg ctttagacaa atcattgaaa
     3061 agaacacctg gaaaagtgac agcaaacaac ttgttgatga agctcaatct gaagtatggt
     3121 ggcgtgaatc acaaagtgcg agtggacaat agcatttctc atctctgggg agacacttca
     3181 aacacactca tcatctcata tgatgtgtgc cattcttctg gaaaagtgta caagaaggat
     3241 gagatttgtg acgagccaag ctgcgttggt tttggattca atggaaccgc ttgccccgaa
     3301 gcattcattg gagatttcca ttaccagctc ccacgtcacg aacaagttga tgaacatctt
     3361 ctgaaattga gagccagatt catgctcaat cactatatta catctcgtaa gaagtatccc
     3421 caacaagtgg tcattttgcg cgacggagtc tctgaaggac aacacaaaat ggtcagaaac
     3481 gaagagttcc aggcgattaa acagagcatc atgaatgttt tcaatgaaaa gaaggcaaag
     3541 gctccgtctt ttgctcttct cgttgtcact aagcgtcatg ccaatcggct tttgattaaa
     3601 gatcagaatg gatatacgaa tgtcccaccg ttgacggcaa ttgaccaaac aattgtgaag
     3661 aagcaaggaa atgaagtcat ctttgtgtcc cattgtccac tcaatggtac cgcccaacca
     3721 attgtaatca atacattggt caacgatgga attttcaaga ccaatgatca attggtccag
     3781 ttcatcacag cactttgctg tgctcatcag aagtctacaa acatagtgtc tctcccagag
     3841 agtatctacg cagctgacga atacgccaaa agaggagccg atttgttcca gtcgtataaa
     3901 ctgaagcatc gagatcttcc aacgactacg atcgacgact cgacccagct cgactacgaa
     3961 aaaatcaccc taattctctg cttccaaacg agctctttca aaaataagag aattgcttaa
     4021 cccgggtcga ctgcagcgat cgctcgagca ccaccaccac caccactgag atccggctgc
     4081 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     4141 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     4201 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4741 tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat
     4801 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     4861 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     4921 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     4981 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     5041 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     5101 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     5161 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     5221 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     5281 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     5341 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     5401 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     5461 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     5521 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
     5581 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc
     5641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5821 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     5881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6361 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6421 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     6481 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     6541 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     6601 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6661 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6721 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     6781 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     6841 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     6901 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     6961 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     7021 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     7081 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     7141 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     7201 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     7261 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     7321 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     7381 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     7441 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     7501 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     7561 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     7621 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     7681 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7801 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     7861 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     7921 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     7981 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     8041 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     8101 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     8161 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     8221 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     8281 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     8341 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     8401 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     8461 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     8521 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     8581 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     8641 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     8701 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     8761 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     8821 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     8881 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     8941 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     9001 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     9061 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     9121 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     9181 aaat
//
