LOCUS       sequence-403825-        9239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate mCherry-PAR-6B (mouse) expressing stable cell lines with
            piggybac transposase co-expression and hygromycin selections..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Liang X, Weberling A, Hii CY, Zernicka-Goetz M, Buckley CE
  TITLE     E-cadherin mediates apical membrane initiation site localisation 
            during de novo polarisation of epithelial cavities.
  JOURNAL   EMBO J. 2022 Aug 22:e111021. doi: 10.15252/embj.2022111021.
  PUBMED    35993232
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2022 Aug 22:e111021. doi: 10.15252/embj.2022111021."
COMMENT     Sequence Label: PB-Hyg-Dest-mCherry-PAR-6B
FEATURES             Location/Qualifiers
     source          1..9239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(156..655)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(204..223)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     597..620
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    678..711
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        727..1106
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1108..1385
                     /label=chicken beta-actin promoter
     intron          1386..2401
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2325..2347
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2409..2428
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2479..2503
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2506..2515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2512..3219
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPMQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2657..2675)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2925..2944
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4171..4195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4216..4232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4240..4256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4264..4270,4271..4288,4289..4294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4309..4330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4390..4407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4396..4620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4697..4730
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   4901..4963
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(4987..5005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5026..5042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5050..5066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5074..5080,5081..5098,5099..5104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5119..5140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5257..5274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5428..6016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5508..5527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6187..6978,6979..7047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6810..6829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7048..7152)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7178..7633)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7315..7336)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7527..7546
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7760..7782
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7774..7791
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7775..7791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7798..7817
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7798..7816
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7835..7869
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(join(8351..9239,1..137))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(join(8351..9239,1..137))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
       61 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
      121 agttcaggct ttttcatggt ggcggctgga tcggtcgaaa ggcccggaga tgaggaagag
      181 gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac
      241 cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt
      301 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
      361 cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct
      421 acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact
      481 aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta
      541 ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc
      601 ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag
      661 aattcatcga tgatatcata acttcgtata atgtatgcta tacgaagtta tgcggccctc
      721 gaggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      781 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      841 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      901 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      961 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     1021 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     1081 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     1141 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     1201 cagcgrtggg ggsggggggg gggggggggc gcgcgccrgg sggggsgggg sggggcgrgg
     1261 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1321 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1381 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1441 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     1501 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     1561 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     1621 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     1681 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1741 ccgggggcgg tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1801 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1861 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1921 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1981 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     2041 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     2101 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     2161 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     2221 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     2281 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2341 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2401 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga
     2461 cggtatcgat aagctatcac aagtttgtac aaaaaagcag gcttcgccac catggtgagc
     2521 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2581 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2641 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     2701 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     2761 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2821 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2881 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2941 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3001 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3061 accacctaca aggccaagaa gcccatgcag ctgcccggcg cctacaacgt caacatcaag
     3121 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3181 ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct
     3241 caagcttcga attcctgcag cccgggggat ccgatgaacc gcggccaccg gcacggggcg
     3301 agcagcggct ggctgggcac catggaggtg aagagcaagt ttggagctga gtttcgtcgg
     3361 ttttcactgg aaagatctaa gcctggaaaa tttgaggagt tttacggact gctgcaacat
     3421 gttcacaaga tccccaatgt cgacgtgttg gtgggctacg cagatatcca cggggacctg
     3481 ctgcctataa ataatgatga caactaccac aaggcggttt ccaccgccaa tccactgctc
     3541 aggattttta tacagaagaa ggaagaagct gactacagtg cctttggcac cgacaccctg
     3601 atcaggaaga agaacatgct gagcaacgtg ctgcgtcccg acaaccaccg gaagaagcct
     3661 cacatcgtca tcagcatgcc gcaggacttc cggcccgtgt catccatcat cgacgtggac
     3721 atcctcccag agacgcaccg gagggtccgt ctgtacaagt acggcacaga gaagccgctg
     3781 ggcttctaca tccgggatgg ctccagcgtc cgagtgacac cgcacggctt ggagaaagtc
     3841 cccggcatct tcatatctcg gctcgtccct gggggtctgg cccagagcac gggcctgcta
     3901 gctgtcaacg atgaggtgtt agaagtcaac ggtatagaag tgtccgggaa gagcctggac
     3961 caggtgactg acatgatgat agccaacagc cgcaacctca tcatcaccgt gcgcccggcc
     4021 aaccagagga acaacgtggt gcgcaacagt cggacttctg gcagctccag ccagtccact
     4081 gacaacagcc tcctgggctt cccacagcag gtggaggcca gcttcgagcc cgaggaccag
     4141 gacagcgacg aggacgacat catctgctag acccagcttt cttgtacaaa gtggtgatag
     4201 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4261 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4321 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4381 gcggatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4441 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4501 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4561 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4621 cttctgaggc ggaaagaacc agctggggct cgagatccac tagttctagc ctcgaggcta
     4681 gagcggccgc gtcgagataa cttcgtataa tgtatgctat acgaagttat gttttgttac
     4741 tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat
     4801 tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc
     4861 aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat
     4921 gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaaaga gctccagctt
     4981 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     5041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7141 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     7201 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7261 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     7321 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     7381 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     7441 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     7501 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     7561 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     7621 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7681 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     7741 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     7801 tacgactcac tatagggcga attgggtacc ttaaccctag aaagatagtc tgcgtaaaat
     7861 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
     7921 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt
     7981 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
     8041 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
     8101 acgtgataac ttattatata tatattttct tgttatagat atccaactat gaaacattat
     8161 cataaagcaa tagtaaagag ccttcagtaa agagcaggca tttatctaat cccaccccac
     8221 ccccaccccc gtagctccaa tccttccatt caaaatgtag gtactctgtt ctcacccttc
     8281 ttaacaaagt atgacaggaa aaacttccat gtggacatct ttattgttta atagatcatc
     8341 aatttctcga ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     8401 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     8461 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     8521 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     8581 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     8641 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     8701 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     8761 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     8821 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     8881 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     8941 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     9001 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     9061 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     9121 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     9181 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggaca
//
